Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0303
(663 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 223 1e-59
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 102 5e-23
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 66 4e-12
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 56 3e-09
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 51 1e-07
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 44 2e-05
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 39 7e-04
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 34 0.021
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 33 0.037
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 32 0.085
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 25 7.4
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 223 bits (546), Expect = 1e-59
Identities = 101/173 (58%), Positives = 129/173 (74%)
Frame = +3
Query: 141 GFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQK 320
GF FE +QRN L +KGFP PKAT TGTTIVG+I D ++LGADTRAT +++DKNC+K
Sbjct: 9 GFDFEYYQRNLLLQEKGFPTPKATSTGTTIVGVIAKDCIVLGADTRATAGPIIADKNCKK 68
Query: 321 IHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGH 500
+H ++ N++C GAGTAADTE T ++S +EL L+T R V TA T+LK+ LFRYQGH
Sbjct: 69 LHLISPNIWCAGAGTAADTEFVTSMISSNIELHSLYTNRKPRVVTALTMLKQHLFRYQGH 128
Query: 501 IGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 659
IGA LVLGG D GPH++ I HGS DKLPY +GSGSLAA++V E ++ D+
Sbjct: 129 IGAYLVLGGYDCKGPHLFTIAAHGSSDKLPYVALGSGSLAAISVLETKYQPDL 181
>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 102 bits (244), Expect = 5e-23
Identities = 53/150 (35%), Positives = 82/150 (54%), Gaps = 1/150 (0%)
Frame = +3
Query: 213 KTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQ 392
+ GTTI + Y DGVIL AD+R T ++++ K+ L N++CC +G+AADT+
Sbjct: 22 RMGTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVAD 81
Query: 393 SVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVD-RTGPHIYCIYPH 569
+ L + R+ G V TAATL M ++ + + A L++ G D +TG +Y I
Sbjct: 82 LLKYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNMLSAGLIVAGYDEKTGGDVYSIPLG 141
Query: 570 GSVDKLPYATMGSGSLAAMAVFEAGWKRDM 659
GS+ K P A GSGS +A ++ +M
Sbjct: 142 GSLHKQPLAIGGSGSAFIYGFCDANFRENM 171
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 66.1 bits (154), Expect = 4e-12
Identities = 46/181 (25%), Positives = 85/181 (46%), Gaps = 3/181 (1%)
Frame = +3
Query: 129 IPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDK 308
+PVP S + RN K GTT + Y G+++ D+RA+ +++ +
Sbjct: 33 VPVPQSSL--YLRNLTDETKNKHCLIKMNHGTTTLAFRYQHGIVVCVDSRASAGPLIASQ 90
Query: 309 NCQKIHYLASNMYCCG--AGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRML 482
+K+ + N Y G AG AAD + + + L +L + V A+ +L +
Sbjct: 91 TVKKV--IEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISVSAASKILSNIT 148
Query: 483 FRYQGH-IGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 659
+ Y+G+ + +L G + G +Y I G+ K ++GSGS A V ++G++ D+
Sbjct: 149 YSYKGYGLSMGTMLAGTGKGGTALYYIDSDGTRLKGDLFSVGSGSTFAYGVLDSGYRWDL 208
Query: 660 N 662
+
Sbjct: 209 S 209
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 56.4 bits (130), Expect = 3e-09
Identities = 40/165 (24%), Positives = 74/165 (44%), Gaps = 11/165 (6%)
Frame = +3
Query: 198 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 377
A +A K G+T +G+ D V+LG + R T + ++ + +K+ + S++ C +G AD
Sbjct: 27 AIEAIKLGSTAIGVKTKDAVVLGVEKRLT-SPLMESHSVEKLFEIDSHIGCAISGLTADA 85
Query: 378 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRY-QGH----------IGAALVLG 524
+ Q + R +E+ + + R+ +G G AL++
Sbjct: 86 RTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVALLIA 145
Query: 525 GVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 659
G+D GP +Y P G+ + +GSGS A + + +DM
Sbjct: 146 GIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDM 190
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 51.2 bits (117), Expect = 1e-07
Identities = 36/151 (23%), Positives = 70/151 (46%), Gaps = 7/151 (4%)
Frame = +3
Query: 231 VGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQL 410
+GI+ DG++L A+ + T + +++ +K++++ NM C AG AD + + A ++
Sbjct: 35 LGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILI-NYARRV 93
Query: 411 ELQRLHT-GRTVPVETAATL---LKRMLFRYQG--HIGAALVLGGVDR-TGPHIYCIYPH 569
Q L T +P E LK+ +Y G G + + G D G ++ P
Sbjct: 94 GQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPS 153
Query: 570 GSVDKLPYATMGSGSLAAMAVFEAGWKRDMN 662
G+ ++G S + ++ +K D+N
Sbjct: 154 GNYGSWQANSIGGNSTSVQSLMRQEYKDDIN 184
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 44.0 bits (99), Expect = 2e-05
Identities = 38/178 (21%), Positives = 72/178 (40%), Gaps = 6/178 (3%)
Frame = +3
Query: 144 FSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKI 323
FS F N L Q + A A G T VGI DGV+L + + T + + +K+
Sbjct: 7 FSLTTFSPNGKLVQIEY-ALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIG-ASLEKV 64
Query: 324 HYLASNMYCCGAGTAADTEMTTQSV--ASQLELQRLHTG----RTVPVETAATLLKRMLF 485
+ ++ +G D + +Q ++++ + + E A+ + +
Sbjct: 65 CAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQS 124
Query: 486 RYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 659
G +L++ G+D GP +Y + P G+ +G S AA E + ++
Sbjct: 125 GGVRPFGVSLLVAGMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDEL 182
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 38.7 bits (86), Expect = 7e-04
Identities = 29/132 (21%), Positives = 61/132 (46%), Gaps = 4/132 (3%)
Frame = +3
Query: 201 PKATKTGTTIVGIIYADG-VILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 377
P GTT+ I DG IL DTR+ ++ + ++H + ++ +G AD
Sbjct: 8 PYVQNGGTTVA--IAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADA 65
Query: 378 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLF--RYQGHIGAALVLGGVDRTGP-H 548
+ + +++L + R + ++ A +++ +L+ R+ + + G+D+ G
Sbjct: 66 LALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYY-VYTTVAGIDKEGKGE 124
Query: 549 IYCIYPHGSVDK 584
IY P GS ++
Sbjct: 125 IYSFDPVGSYER 136
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 33.9 bits (74), Expect = 0.021
Identities = 35/147 (23%), Positives = 54/147 (36%), Gaps = 5/147 (3%)
Frame = +3
Query: 198 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 377
A KA + +T +GI DGVIL + T + N +I + ++ G D
Sbjct: 26 AYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVN-NRIGSVDRHIGIATTGFIPDG 84
Query: 378 EMTTQSVASQLELQRLHTGRTVPVETAATLLKR--MLFRYQGHI---GAALVLGGVDRTG 542
+ + + R + G +P A L LF + G + D G
Sbjct: 85 QHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSEG 144
Query: 543 PHIYCIYPHGSVDKLPYATMGSGSLAA 623
PH+Y + P+G A G G A
Sbjct: 145 PHLYMVEPNGVYWGYNGAAAGKGRQVA 171
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 33.1 bits (72), Expect = 0.037
Identities = 35/152 (23%), Positives = 62/152 (40%), Gaps = 5/152 (3%)
Frame = +3
Query: 198 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 377
A +A K G+ VG++ +L A R E K K+ + ++ AG A D
Sbjct: 25 ALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQK---KLIRIDDHIGIAIAGLAPDA 81
Query: 378 EMTTQSVASQLELQRLHTGRTVPVETAAT-LLKRMLFRYQGH----IGAALVLGGVDRTG 542
+ + + + + R +PV + + ++ Q + G ++ G D +G
Sbjct: 82 RVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIGYDESG 141
Query: 543 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFE 638
PH+ P G V + +MGS S +A E
Sbjct: 142 PHLLEFQPSGLVLEYLGTSMGSRSQSARTYIE 173
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 31.9 bits (69), Expect = 0.085
Identities = 16/73 (21%), Positives = 39/73 (53%)
Frame = +3
Query: 216 TGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQS 395
TG++++ + +ADGV++ AD A+ ++ + +++ + N G +D + Q
Sbjct: 42 TGSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQ-IQR 100
Query: 396 VASQLELQRLHTG 434
+ +LE++ + G
Sbjct: 101 LLEKLEIKEGNYG 113
>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 995
Score = 25.4 bits (53), Expect = 7.4
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = -2
Query: 560 NAVNMRTGSVNTSQNQSSPYMALIAK*HSFQQCGSSF 450
N + M+ S+ T + + +PY L+A + CGS +
Sbjct: 861 NIIPMQKPSLTTHETKQNPYDLLVALDSDRKACGSLY 897
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,745,457
Number of Sequences: 5004
Number of extensions: 55522
Number of successful extensions: 127
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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