Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0291
(409 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g78370.1 68414.m09133 glutathione S-transferase, putative sim... 35 0.018
At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 33 0.097
At1g78380.1 68414.m09134 glutathione S-transferase, putative sim... 31 0.22
At1g56610.1 68414.m06511 syntaxin-related family protein similar... 30 0.69
At1g49860.1 68414.m05590 glutathione S-transferase, putative sim... 30 0.69
At5g17220.1 68418.m02018 glutathione S-transferase, putative 29 1.2
At1g17190.1 68414.m02095 glutathione S-transferase, putative One... 29 1.6
At1g10600.1 68414.m01200 mov34 family protein similar to AMSH [H... 28 2.1
At3g07900.1 68416.m00965 expressed protein contains Pfam PF03138... 28 2.8
At1g17780.1 68414.m02201 expressed protein 28 2.8
At1g53680.1 68414.m06108 glutathione S-transferase, putative sim... 27 3.7
At5g07200.1 68418.m00820 gibberellin 20-oxidase identical to GI:... 27 4.8
At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 27 4.8
At5g44360.1 68418.m05432 FAD-binding domain-containing protein s... 27 6.4
At4g16950.2 68417.m02557 disease resistance protein (TIR-NBS-LRR... 27 6.4
At4g16950.1 68417.m02556 disease resistance protein (TIR-NBS-LRR... 27 6.4
At2g42010.1 68415.m05197 phospholipase D beta 1 / PLD beta 1 (PL... 27 6.4
At1g33540.1 68414.m04150 serine carboxypeptidase S10 family prot... 27 6.4
At5g36270.1 68418.m04375 dehydroascorbate reductase, putative si... 26 8.5
At5g06810.1 68418.m00770 mitochondrial transcription termination... 26 8.5
At4g20860.1 68417.m03026 FAD-binding domain-containing protein s... 26 8.5
At3g23430.1 68416.m02953 phosphate transporter, putative (PHO1) ... 26 8.5
>At1g78370.1 68414.m09133 glutathione S-transferase, putative
similar to 2,4-D inducible glutathione S-transferase
GI:2920666 from [Glycine max]
Length = 217
Score = 35.1 bits (77), Expect = 0.018
Identities = 22/78 (28%), Positives = 31/78 (39%), Gaps = 5/78 (6%)
Frame = -1
Query: 409 WGETTFKPQ-GLAKIKSAYDFTEKFLSDSPWIAGDDVTVADMSCVATIGSLDALLPIN-- 239
WG+ + + G + A E L D P+ GD D+S + A
Sbjct: 114 WGKKGEEQEAGKKEFIEAVKILESELGDKPYFGGDSFGYVDISLITFSSWFQAYEKFGNF 173
Query: 238 --EKEYPKITSWLKRCSE 191
E E PK+ +W KRC E
Sbjct: 174 SIESESPKLIAWAKRCME 191
>At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6)
identical to GB:X95295. Based on identical cDNA hits,
the translation is now 40 AAs longer at the N-terminal,
and start of exon2 is also corrected.
Length = 263
Score = 32.7 bits (71), Expect = 0.097
Identities = 19/63 (30%), Positives = 35/63 (55%), Gaps = 3/63 (4%)
Frame = -1
Query: 373 KIKSAYDFTEKFLSDSPWIAGDDVTVADMSCVATIGSL---DALLPINEKEYPKITSWLK 203
K++ D E L+ S ++AGD T+AD+ + I L D+ + + + PK++ W+K
Sbjct: 189 KLQKVLDVYEARLAKSEFLAGDSFTLADLHHLPAIHYLLGTDSKVLFDSR--PKVSEWIK 246
Query: 202 RCS 194
+ S
Sbjct: 247 KIS 249
>At1g78380.1 68414.m09134 glutathione S-transferase, putative
similar to glutathione transferase GI:2853219 from
[Carica papaya]
Length = 219
Score = 31.5 bits (68), Expect = 0.22
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 4/54 (7%)
Frame = -1
Query: 346 EKFLSDSPWIAGDDVTVADMSCVATIGSLDALLPIN----EKEYPKITSWLKRC 197
E L D P+ +GDD D++ + A E E PK+ +W+K+C
Sbjct: 136 ESELGDKPYFSGDDFGYVDIALIGFYTWFPAYEKFANFSIESEVPKLIAWVKKC 189
>At1g56610.1 68414.m06511 syntaxin-related family protein similar to
proteins At3g54160, At1g47920 (syntaxin SYP81),
At5g41830, At3g44180, At3g58890, At1g48390, At3g59270
[Arabidopsis thaliana]; contains a novel domain similar
to F-box that is shared among other proteins in
Arabidopsis
Length = 535
Score = 29.9 bits (64), Expect = 0.69
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = -1
Query: 322 WIAGDDVTVADMSCVATIGSLDALLPINEKEYPKITSWLKRCSELD 185
WI GDDV A + C+ T+ LD +L + K + ++ L RC L+
Sbjct: 213 WINGDDVAAAHLPCLKTLW-LDDVLVADVKVFVRL---LSRCPILE 254
>At1g49860.1 68414.m05590 glutathione S-transferase, putative
similar to GI:860955 from [Hyoscyamus muticus] (Plant
Physiol. 109 (1), 253-260 (1995))
Length = 254
Score = 29.9 bits (64), Expect = 0.69
Identities = 18/65 (27%), Positives = 35/65 (53%), Gaps = 7/65 (10%)
Frame = -1
Query: 373 KIKSAYDFTEKFLSDSPWIAGDDVTVADMSCVATIGSLDALLPINEKEY-------PKIT 215
K+ + E L +SP++AG+ ++AD+ +A I D LL +E+E P +
Sbjct: 142 KLSEVLNIYETRLGESPYLAGESFSLADLHHLAPI---DYLLNTDEEELKNLIYSRPNVA 198
Query: 214 SWLKR 200
+W+++
Sbjct: 199 AWVEK 203
>At5g17220.1 68418.m02018 glutathione S-transferase, putative
Length = 214
Score = 29.1 bits (62), Expect = 1.2
Identities = 16/63 (25%), Positives = 32/63 (50%), Gaps = 2/63 (3%)
Frame = -1
Query: 373 KIKSAYDFTEKFLSDSPWIAGDDVTVADMSCVATIGSLDALLPINE--KEYPKITSWLKR 200
K+ D LS + ++AG++ T+AD++ + +G L ++ IN+ K W +
Sbjct: 139 KLGVVLDIYNNRLSSNRFLAGEEFTMADLTHMPAMGYLMSITDINQMVKARGSFNRWWEE 198
Query: 199 CSE 191
S+
Sbjct: 199 ISD 201
>At1g17190.1 68414.m02095 glutathione S-transferase, putative One of
three repeated glutathione transferases. 65% identical
to glutathione transferase [Arabidopsis thaliana]
(gi|4006934). Location of est 141C5T7 (gb|T46669);
supported by fl cDNA gi:14326476gb:AF385691.
Length = 220
Score = 28.7 bits (61), Expect = 1.6
Identities = 16/56 (28%), Positives = 25/56 (44%), Gaps = 4/56 (7%)
Frame = -1
Query: 346 EKFLSDSPWIAGDDVTVADMSCVATIGSLDALLPINE----KEYPKITSWLKRCSE 191
E L D P+ G+ D++ + A+ E E+P +T+W KRC E
Sbjct: 137 ETELGDKPYYGGEVFGYLDIALMGYYSWFKAMEKFGEFSIETEFPILTTWTKRCLE 192
>At1g10600.1 68414.m01200 mov34 family protein similar to AMSH [Homo
sapiens] GI:4098124; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 271
Score = 28.3 bits (60), Expect = 2.1
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 111 PRLKYCFSNALNSNRPFTLFPLW*KSSSEQR-FNH 212
P ++C + NSN F L+PLW K + R NH
Sbjct: 201 PVYEHCSNVYKNSNLRFKLYPLWAKDVNNNRHHNH 235
>At3g07900.1 68416.m00965 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 579
Score = 27.9 bits (59), Expect = 2.8
Identities = 16/53 (30%), Positives = 25/53 (47%)
Frame = +3
Query: 66 VIASDYAIIDFFYSFPRLKYCFSNALNSNRPFTLFPLW*KSSSEQRFNHEVIL 224
V+A+D I+ P K + + + PF P W +S +RFN E +L
Sbjct: 250 VLANDVKIVSLL---PASKVMTRPSEDGSMPFNASPQWIRSHYPKRFNREGVL 299
>At1g17780.1 68414.m02201 expressed protein
Length = 189
Score = 27.9 bits (59), Expect = 2.8
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +1
Query: 79 TMQSSIFFILFHDSSIVSVTP*IPIGL-LRCF 171
T Q F +LFHD S++ + +P+ L L CF
Sbjct: 149 TKQLRSFKVLFHDVSVIKLNTVLPVSLFLICF 180
>At1g53680.1 68414.m06108 glutathione S-transferase, putative
similar to GI:2853219 from [Carica papaya]
Length = 224
Score = 27.5 bits (58), Expect = 3.7
Identities = 22/79 (27%), Positives = 30/79 (37%), Gaps = 6/79 (7%)
Frame = -1
Query: 409 WGETTFKPQ--GLAKIKSAYDFTEKFLSDSPWIAGDDVTVADMSCVATIGSLDALLPIN- 239
WG + Q G + + E L D + G+ D++ V AL
Sbjct: 118 WGNKKGEEQEKGKKEFLESLKVLEAELGDKSYFGGETFGYVDITLVPFYSWFYALEKCGD 177
Query: 238 ---EKEYPKITSWLKRCSE 191
E E PKI +W KRC E
Sbjct: 178 FSVEAECPKIVAWGKRCVE 196
>At5g07200.1 68418.m00820 gibberellin 20-oxidase identical to
GI:1109699
Length = 380
Score = 27.1 bits (57), Expect = 4.8
Identities = 15/47 (31%), Positives = 25/47 (53%)
Frame = +3
Query: 225 GYSFSLIGKSASKLPIVATQLISATVTSSPAIHGESERNFSVKS*AD 365
GY+ S +G+ +SKLP T +S + IH ++ ++F K D
Sbjct: 133 GYASSFVGRFSSKLPWKET--LSFKFSPEEKIHSQTVKDFVSKKMGD 177
>At2g30860.1 68415.m03761 glutathione S-transferase, putative
identical to GB:Y12295
Length = 215
Score = 27.1 bits (57), Expect = 4.8
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -1
Query: 373 KIKSAYDFTEKFLSDSPWIAGDDVTVADMS 284
K+ D E LS S ++AGD V++AD++
Sbjct: 138 KLAGVLDVYEAHLSKSKYLAGDFVSLADLA 167
>At5g44360.1 68418.m05432 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 532
Score = 26.6 bits (56), Expect = 6.4
Identities = 8/28 (28%), Positives = 19/28 (67%)
Frame = -1
Query: 271 IGSLDALLPINEKEYPKITSWLKRCSEL 188
+G +D L+P+ +++P++ + CSE+
Sbjct: 313 LGGIDKLIPLMNQKFPELGLTFQDCSEM 340
>At4g16950.2 68417.m02557 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.; closest
homolog in Col-0 to RPP5 of clutivar Landsberg erecta.
Length = 1404
Score = 26.6 bits (56), Expect = 6.4
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +1
Query: 160 LRCFLSGKSLAQNSVSTTKLSWD 228
LR FL+ KS + + VS KLSW+
Sbjct: 239 LRAFLTYKSTSGSDVSGMKLSWE 261
>At4g16950.1 68417.m02556 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.; closest
homolog in Col-0 to RPP5 of clutivar Landsberg erecta.
Length = 1449
Score = 26.6 bits (56), Expect = 6.4
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +1
Query: 160 LRCFLSGKSLAQNSVSTTKLSWD 228
LR FL+ KS + + VS KLSW+
Sbjct: 239 LRAFLTYKSTSGSDVSGMKLSWE 261
>At2g42010.1 68415.m05197 phospholipase D beta 1 / PLD beta 1
(PLDBETA1) identical to SP|P93733 Phospholipase D beta 1
(EC 3.1.4.4) (AtPLDbeta1) (PLD beta 1) (PLDbeta)
{Arabidopsis thaliana}; contains Pfam profiles: PF00614
phospholipase D.active site motif, PF00168 C2 domain
Length = 1083
Score = 26.6 bits (56), Expect = 6.4
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +2
Query: 164 VVSSLVKV*LRTAFQPRSYLGILFLINRQERIQTADCSNTAHISHSD 304
+ +LV+ L AF P+ YL L NR E + D S T S+++
Sbjct: 872 IYKALVETGLEGAFSPQDYLNFFCLGNR-EMVDGIDNSGTGSPSNAN 917
>At1g33540.1 68414.m04150 serine carboxypeptidase S10 family protein
similar to GI:8777303 from [Arabidopsis thaliana] (DNA
Res. 7 (1), 31-63 (2000))
Length = 446
Score = 26.6 bits (56), Expect = 6.4
Identities = 9/34 (26%), Positives = 19/34 (55%)
Frame = -1
Query: 397 TFKPQGLAKIKSAYDFTEKFLSDSPWIAGDDVTV 296
++KP + K Y+F +K+L ++P + + V
Sbjct: 143 SYKPSDTGEAKQTYEFLQKWLVENPQFVSNPIYV 176
>At5g36270.1 68418.m04375 dehydroascorbate reductase, putative
similar to dehydroascorbate reductase {Spinacia
oleracea} gi:10952511 gb:AF195783, PMID:11148269
Length = 217
Score = 26.2 bits (55), Expect = 8.5
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -1
Query: 325 PWIAGDDVTVADMSCVATIGSLDALL 248
P++AGD VTV D+S + L+ L
Sbjct: 144 PFVAGDKVTVVDLSLAPKLYHLETTL 169
>At5g06810.1 68418.m00770 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 1141
Score = 26.2 bits (55), Expect = 8.5
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = +3
Query: 75 SDYAIIDFFYSFPRLKYCFSNALNSNRP 158
++ A++D+FYS L+Y + +++ N P
Sbjct: 78 AEEAMLDYFYSTRGLQYMVAESMSKNSP 105
>At4g20860.1 68417.m03026 FAD-binding domain-containing protein
simlar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 530
Score = 26.2 bits (55), Expect = 8.5
Identities = 8/32 (25%), Positives = 21/32 (65%)
Frame = -1
Query: 271 IGSLDALLPINEKEYPKITSWLKRCSELDFYQ 176
+G +D L+P+ +++P++ + CSE+ + +
Sbjct: 312 LGGIDRLIPLMNQKFPELGLRSQDCSEMSWIE 343
>At3g23430.1 68416.m02953 phosphate transporter, putative (PHO1)
identical to PHO1 protein [Arabidopsis thaliana]
GI:20069032; supporting cDNA gi|20069031|gb|AF474076.1|;
contains Pfam profiles PF03124: EXS family and PF03105:
SPX domain
Length = 782
Score = 26.2 bits (55), Expect = 8.5
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = -1
Query: 238 EKEYPKITSWLKRCSELDFYQRGNNVKGLLEFKALLKQYLSRGKE 104
++E K+ + K E +F +RG +K LE A LKQ LS K+
Sbjct: 123 DEELNKVNQFHKP-KETEFLERGEILKKQLETLAELKQILSDRKK 166
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,595,027
Number of Sequences: 28952
Number of extensions: 142822
Number of successful extensions: 438
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 431
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 436
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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