BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0277 (593 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|c... 73 4e-14 SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosacc... 29 0.68 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 28 1.2 SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosac... 25 8.3 >SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 240 Score = 72.5 bits (170), Expect = 4e-14 Identities = 40/133 (30%), Positives = 70/133 (52%) Frame = +2 Query: 191 KDAERSFKWRNCALSQQILQEPIVACSLGRLYSKSSVLEALLDKETRPESINHXXXXXXX 370 K + +S ++ CA++ + L PIV+C LG+LY+K+S+L+ LLD+ + P+S +H Sbjct: 29 KRSVKSSQFSQCAITDEPLYPPIVSCGLGKLYNKASILQMLLDRSSVPKSPSH------- 81 Query: 371 XXXXXXXXPAYVPTDHTDGTFDNGSAPYICPISGLEMTGKFRFVFLWSCGCVLAERALKE 550 V D D+G ++CPI+ M+ ++F ++ CG V ALK+ Sbjct: 82 IKSLKDVVQLQVELD------DSGKVLWLCPITRHVMSDTYQFAYIVPCGHVFEYSALKQ 135 Query: 551 VRQNLCHMCQQPF 589 + +C C Q + Sbjct: 136 FGEKMCFQCNQVY 148 Score = 27.5 bits (58), Expect = 1.6 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 2/34 (5%) Frame = +3 Query: 120 MGCDGGTIPRRDELVRM--KKKPEQIKMLKEVSS 215 MG DGG++P R+ELV+ K P I + V S Sbjct: 1 MGNDGGSLPTRNELVKEPGKVPPLDIDFKRSVKS 34 >SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosaccharomyces pombe|chr 1|||Manual Length = 636 Score = 28.7 bits (61), Expect = 0.68 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = +2 Query: 440 GSAPYICPISGLEMTGKFRFVFLWSCGCV 526 GSAPYI P E R V +W+CG + Sbjct: 522 GSAPYIAPEEYTESEFDPRAVDVWACGVI 550 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 27.9 bits (59), Expect = 1.2 Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 4/29 (13%) Frame = +2 Query: 515 CGCVLAERALKEVRQN----LCHMCQQPF 589 CG L+E + R+N LCH C+QPF Sbjct: 898 CGDCLSEHIQYQKRRNIIPPLCHTCRQPF 926 >SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 250 Score = 25.0 bits (52), Expect = 8.3 Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 1/50 (2%) Frame = +2 Query: 167 NEKEARTDKDAERSFKWRN-CALSQQILQEPIVACSLGRLYSKSSVLEAL 313 N +E D+ S + N C L+ Q + PI++ + Y K ++L L Sbjct: 159 NTEEQEADEVMVYSATFDNRCPLTLQPIVHPILSTACNHFYEKDAILSLL 208 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,521,935 Number of Sequences: 5004 Number of extensions: 49916 Number of successful extensions: 162 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 156 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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