Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0254
(408 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 209 1e-55
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 102 2e-23
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 97 9e-22
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 78 5e-16
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 52 4e-12
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 64 6e-12
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 56 2e-09
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 56 3e-09
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 33 0.023
SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 27 1.5
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 26 2.6
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 25 4.5
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 25 4.5
SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar... 25 6.0
SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|... 25 6.0
SPCC1259.07 |||transcriptional regulatory protein Rxt3 |Schizosa... 25 6.0
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 25 6.0
SPAPB24D3.01 ||SPAPB2C8.02|transcription factor |Schizosaccharom... 24 7.9
SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 24 7.9
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 24 7.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 209 bits (510), Expect = 1e-55
Identities = 97/112 (86%), Positives = 102/112 (91%)
Frame = +1
Query: 73 TAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNFIMKCDVD 252
TAA SSSLEKSYELPDGQVITIGNERFR PEALFQPS LG+E GIHE TYN IMKCDVD
Sbjct: 229 TAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVD 288
Query: 253 IRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVW 408
IRKDLY N V+SGGTTMYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVW
Sbjct: 289 IRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVW 340
Score = 35.1 bits (77), Expect = 0.004
Identities = 19/27 (70%), Positives = 20/27 (74%)
Frame = +2
Query: 26 REKLCYVALDFEQEMAPLHPAAPSRSL 106
+EKLCYVALDFEQE L AA S SL
Sbjct: 213 KEKLCYVALDFEQE---LQTAAQSSSL 236
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 102 bits (245), Expect = 2e-23
Identities = 44/102 (43%), Positives = 64/102 (62%)
Frame = +1
Query: 103 SYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNFIMKCDVDIRKDLYANTV 282
+Y+LPD Q IT+G E F PE LF P +G EA G+H + I+ D+D+R LY+N V
Sbjct: 244 TYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIV 303
Query: 283 LSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVW 408
LSGG+T+ G +R E+ A++ ++KI A PER ++ W
Sbjct: 304 LSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAW 345
Score = 24.6 bits (51), Expect = 6.0
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +2
Query: 26 REKLCYVALDFEQEM 70
+EK CYVA DF E+
Sbjct: 218 KEKCCYVASDFRSEI 232
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 97.1 bits (231), Expect = 9e-22
Identities = 44/85 (51%), Positives = 59/85 (69%)
Frame = +1
Query: 94 LEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNFIMKCDVDIRKDLYA 273
L ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E ++ I DVDIRK LY
Sbjct: 236 LMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYR 295
Query: 274 NTVLSGGTTMYPGIADRMQKEITAL 348
VLSGG++MY G+ R++KEI L
Sbjct: 296 AIVLSGGSSMYAGLPSRLEKEIKQL 320
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 78.2 bits (184), Expect = 5e-16
Identities = 40/101 (39%), Positives = 56/101 (55%), Gaps = 9/101 (8%)
Frame = +1
Query: 76 AASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC---------GIHETTYN 228
A +SS K +E PDG G ERFR E LF PSF + G+HE Y
Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330
Query: 229 FIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALA 351
I+ CD ++R L N V++GGT++ PG+++R+Q E+ LA
Sbjct: 331 SILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLA 371
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 52.0 bits (119), Expect(2) = 4e-12
Identities = 28/71 (39%), Positives = 42/71 (59%), Gaps = 3/71 (4%)
Frame = +1
Query: 205 GIHETTYNFIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 384
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 385 ---PERKYSVW 408
ER Y+ W
Sbjct: 385 GHVVERSYASW 395
Score = 33.1 bits (72), Expect(2) = 4e-12
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +1
Query: 76 AASSSSLEKSYELPDGQVITIGNERFRCPEALFQP 180
++ ++ E +E PDG + G ER++ PE LF P
Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 64.5 bits (150), Expect = 6e-12
Identities = 30/89 (33%), Positives = 46/89 (51%)
Frame = +1
Query: 124 QVITIGNERFRCPEALFQPSFLGMEACGIHETTYNFIMKCDVDIRKDLYANTVLSGGTTM 303
QV+ + NERF PE LF PS + + GI E + +I+ L N V GG
Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCK 331
Query: 304 YPGIADRMQKEITALAPSTMKIKIIAPPE 390
+PG R+ E+ +LAP+ ++K+ P +
Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSD 360
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 56.0 bits (129), Expect = 2e-09
Identities = 23/88 (26%), Positives = 47/88 (53%)
Frame = +1
Query: 145 ERFRCPEALFQPSFLGMEACGIHETTYNFIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 324
E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 325 MQKEITALAPSTMKIKIIAPPERKYSVW 408
+++E+T++ P I + W
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAW 681
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 55.6 bits (128), Expect = 3e-09
Identities = 25/71 (35%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
Frame = +1
Query: 130 ITIGNERFRCPEALFQPSFLGME-ACGIHETTYNFIMKCDVDIRKDLYANTVLSGGTTMY 306
I +G ERF PE F P + + E N + +D+RK LY N VLSGG+T++
Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLF 339
Query: 307 PGIADRMQKEI 339
+R+Q+++
Sbjct: 340 KNFGNRLQRDL 350
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 32.7 bits (71), Expect = 0.023
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = +1
Query: 259 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP 387
K+LY++ ++ GG +PG A +++ I + + I +I PP
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPP 569
>SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit
Swi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 722
Score = 26.6 bits (56), Expect = 1.5
Identities = 21/93 (22%), Positives = 38/93 (40%), Gaps = 1/93 (1%)
Frame = +1
Query: 127 VITIGNERFRCPEALFQPSFLG-MEACGIHETTYNFIMKCDVDIRKDLYANTVLSGGTTM 303
++T+ NE + E L S + E+ + + CD+D ++ + + T +
Sbjct: 136 LVTLPNENNQVIEPLSSQSCKSQLSTQNYSESDFGWNQLCDLD---PIFKSLAFTDDTNL 192
Query: 304 YPGIADRMQKEITALAPSTMKIKIIAPPERKYS 402
+P AD I ++K A RKYS
Sbjct: 193 FPAFADSEAALIMLKKREMTRVKHRAGRPRKYS 225
>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 25.8 bits (54), Expect = 2.6
Identities = 12/37 (32%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = +2
Query: 80 HPAAPSRSLTNFPTVRSSPSETKDSV--AQRLSSNPR 184
HPA+ SLTN ++ + P+ D + S +PR
Sbjct: 131 HPASVENSLTNVNSIHAQPTTISDMIDLTDETSYDPR 167
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 25.0 bits (52), Expect = 4.5
Identities = 14/47 (29%), Positives = 22/47 (46%)
Frame = +2
Query: 50 LDFEQEMAPLHPAAPSRSLTNFPTVRSSPSETKDSVAQRLSSNPRSW 190
L EQEM + + + P R + +DS A++L+S SW
Sbjct: 438 LKSEQEMEKQDLSLSWQDTMSNPQDRKFAQDVRDSAARQLTSETPSW 484
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 25.0 bits (52), Expect = 4.5
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +2
Query: 71 APLHPAAPSRSLTNFPTVRSSPSETKDSVAQRLSSNPRS 187
APLHP +P + P +++ P + S+ R + RS
Sbjct: 77 APLHPLSPPSFTHSQPQIQAQPVPRRPSLFDRPNLVSRS 115
>SPBC530.14c |dsk1||SR protein-specific kinase
Dsk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 544
Score = 24.6 bits (51), Expect = 6.0
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = -1
Query: 387 WRSNDLNLHCRWGESCD 337
+RS ++ L CRWG S D
Sbjct: 380 YRSPEVILGCRWGASAD 396
>SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr
3|||Manual
Length = 834
Score = 24.6 bits (51), Expect = 6.0
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +3
Query: 159 PRGSLPTLVLGYGSLRHP 212
P G++PTL+LG G + HP
Sbjct: 568 PLGNVPTLILG-GDVYHP 584
>SPCC1259.07 |||transcriptional regulatory protein Rxt3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 351
Score = 24.6 bits (51), Expect = 6.0
Identities = 13/50 (26%), Positives = 24/50 (48%)
Frame = +2
Query: 23 EREKLCYVALDFEQEMAPLHPAAPSRSLTNFPTVRSSPSETKDSVAQRLS 172
+R+K E+E HP AP+ + + ++ S PS S+++ S
Sbjct: 112 KRQKTLPTQSSGEEEAKYSHPGAPTATSADSISMESRPSNLSTSLSKTTS 161
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 24.6 bits (51), Expect = 6.0
Identities = 9/30 (30%), Positives = 16/30 (53%)
Frame = +1
Query: 145 ERFRCPEALFQPSFLGMEACGIHETTYNFI 234
+ + PE F +LG++ G+H+ FI
Sbjct: 137 DSYNNPELKFWEDYLGLKMEGVHDEVIRFI 166
>SPAPB24D3.01 ||SPAPB2C8.02|transcription factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 594
Score = 24.2 bits (50), Expect = 7.9
Identities = 7/14 (50%), Positives = 8/14 (57%)
Frame = -3
Query: 313 FQGTWWYHRTIRCW 272
F+ WY R I CW
Sbjct: 179 FENVQWYFRYINCW 192
>SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 608
Score = 24.2 bits (50), Expect = 7.9
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +2
Query: 71 APLHPAAPSRSLTNFPTVRSSPSETK 148
AP P P + + PTVR++PS K
Sbjct: 423 APAIPKLPPKHPSRQPTVRATPSTGK 448
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 24.2 bits (50), Expect = 7.9
Identities = 13/55 (23%), Positives = 24/55 (43%)
Frame = +2
Query: 80 HPAAPSRSLTNFPTVRSSPSETKDSVAQRLSSNPRSWVWKLAASTRPHITSS*SA 244
HP + + T+ + SSP+ + SNP W A + P ++ + S+
Sbjct: 405 HPPPWAAASTSVSSSTSSPAPWAKPASSAAPSNPAPWQQPAAPQSAPALSMNPSS 459
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,780,439
Number of Sequences: 5004
Number of extensions: 35818
Number of successful extensions: 139
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 140222766
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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