BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0250 (421 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch... 152 2e-38 SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa... 150 8e-38 SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 32 0.042 SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce... 31 0.073 SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub... 25 3.6 SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 25 4.8 SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 25 4.8 SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 25 4.8 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 25 6.3 SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 25 6.3 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 25 6.3 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 25 6.3 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 24 8.4 SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces pombe... 24 8.4 >SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 3|||Manual Length = 145 Score = 152 bits (368), Expect = 2e-38 Identities = 73/137 (53%), Positives = 97/137 (70%) Frame = +2 Query: 8 SARGKTVK*STTMADVEVEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLHEAAKA 187 S +G ++ + +VEVE +S V AL+EVLK AL+H GL G+ EA+KA Sbjct: 2 SEQGDQIEQVDVVEEVEVEAAAPETVS-----VEDALKEVLKRALVHDGLARGIREASKA 56 Query: 188 LDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKAR 367 LD+RQA LCVL E+CD+ AY KLV+ALC E + PL+KV + K LGEWAGLC +D+DG AR Sbjct: 57 LDRRQAHLCVLCESCDQEAYVKLVEALCAESETPLIKVADPKVLGEWAGLCVLDRDGNAR 116 Query: 368 KIVGCSCVVIKDFGEET 418 K+VGCSCV + D+GE++ Sbjct: 117 KVVGCSCVAVTDYGEDS 133 >SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 150 bits (364), Expect = 8e-38 Identities = 67/107 (62%), Positives = 85/107 (79%) Frame = +2 Query: 98 MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNE 277 + V +L+EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E Sbjct: 30 LSVEDSLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAE 89 Query: 278 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEET 418 Q PLVKV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ Sbjct: 90 SQTPLVKVADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDS 136 >SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 31.9 bits (69), Expect = 0.042 Identities = 17/74 (22%), Positives = 35/74 (47%) Frame = +2 Query: 104 VNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQ 283 +N + + +K A ++ G+ E KA+ K + L +LA + + LC ++ Sbjct: 37 LNKKMMKTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNN 96 Query: 284 IPLVKVDNNKKLGE 325 +P + + + LGE Sbjct: 97 VPYLYTVSKELLGE 110 >SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 31.1 bits (67), Expect = 0.073 Identities = 14/54 (25%), Positives = 27/54 (50%) Frame = +2 Query: 161 HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 322 +GL+ ++ ++A L ++A + D + ALC + +P V N +LG Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187 >SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ubp21|Schizosaccharomyces pombe|chr 2|||Manual Length = 1129 Score = 25.4 bits (53), Expect = 3.6 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +2 Query: 59 VEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLH 172 +E+ P LS D + + VL L+HGG +HG H Sbjct: 445 LEIDLEPYLS-ETADKSESHVYVLHGVLVHGGDLHGGH 481 >SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 25.0 bits (52), Expect = 4.8 Identities = 9/24 (37%), Positives = 13/24 (54%) Frame = -1 Query: 421 WSFLAEIFDDNTGAADNFPCLAIL 350 W +LA +F N A N P + +L Sbjct: 258 WEYLASLFTSNFTAVHNIPSVPLL 281 >SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 25.0 bits (52), Expect = 4.8 Identities = 10/35 (28%), Positives = 19/35 (54%) Frame = +3 Query: 219 WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 323 ++ +V P L+RLC++ R W ++SL+ Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254 >SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccharomyces pombe|chr 1|||Manual Length = 549 Score = 25.0 bits (52), Expect = 4.8 Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%) Frame = -3 Query: 392 QHRSSRQFSLPCHPCQSCRD-QPILQAF 312 + ++ +QF LP PCQ+ + +PI+++F Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 24.6 bits (51), Expect = 6.3 Identities = 15/55 (27%), Positives = 24/55 (43%) Frame = -3 Query: 248 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 84 C+ S +HIV A L +D +DH G+ L ++P V + P+ Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571 >SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor Sec74|Schizosaccharomyces pombe|chr 1|||Manual Length = 928 Score = 24.6 bits (51), Expect = 6.3 Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 1/53 (1%) Frame = -2 Query: 159 TRPPWIKAVFSTSCSATFTSMLLPDKMGLVGTSTSTSA-MVVDYLTVLPRAEF 4 +RP ++F ++ S F+S P L T TST A + L++ P + Sbjct: 148 SRPSSQSSIFLSNWSTIFSSNASPTDSQLSPTHTSTIAELAASTLSIFPSGSY 200 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 24.6 bits (51), Expect = 6.3 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = -3 Query: 155 DHHGLKLSLAPLAVLHSRPC 96 D GL ++ L+++HSRPC Sbjct: 529 DEMGLGKTIEVLSLIHSRPC 548 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 24.6 bits (51), Expect = 6.3 Identities = 15/55 (27%), Positives = 24/55 (43%) Frame = -3 Query: 248 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 84 C+ S +HIV A L +D +DH G+ L ++P V + P+ Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 24.2 bits (50), Expect = 8.4 Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%) Frame = +2 Query: 140 LIHGGLVHGLHEAAKALDKRQAVLCVLAEN-CDE 238 L+H GL+H L + K +LA N CDE Sbjct: 896 LLHSGLIHNLLLSLKKFGSSSLRTFLLAMNTCDE 929 >SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 408 Score = 24.2 bits (50), Expect = 8.4 Identities = 8/21 (38%), Positives = 12/21 (57%) Frame = -3 Query: 71 WGPRLQHQPWLLTI*LSCLVP 9 WGP+ PW+L + S + P Sbjct: 306 WGPKTAWVPWVLGVIYSLVTP 326 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,811,851 Number of Sequences: 5004 Number of extensions: 34699 Number of successful extensions: 131 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 131 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 148351622 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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