BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0250
(421 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch... 152 2e-38
SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa... 150 8e-38
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 32 0.042
SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce... 31 0.073
SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub... 25 3.6
SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 25 4.8
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 25 4.8
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 25 4.8
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 25 6.3
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 25 6.3
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 25 6.3
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 25 6.3
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 24 8.4
SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces pombe... 24 8.4
>SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein
S12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 145
Score = 152 bits (368), Expect = 2e-38
Identities = 73/137 (53%), Positives = 97/137 (70%)
Frame = +2
Query: 8 SARGKTVK*STTMADVEVEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLHEAAKA 187
S +G ++ + +VEVE +S V AL+EVLK AL+H GL G+ EA+KA
Sbjct: 2 SEQGDQIEQVDVVEEVEVEAAAPETVS-----VEDALKEVLKRALVHDGLARGIREASKA 56
Query: 188 LDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKAR 367
LD+RQA LCVL E+CD+ AY KLV+ALC E + PL+KV + K LGEWAGLC +D+DG AR
Sbjct: 57 LDRRQAHLCVLCESCDQEAYVKLVEALCAESETPLIKVADPKVLGEWAGLCVLDRDGNAR 116
Query: 368 KIVGCSCVVIKDFGEET 418
K+VGCSCV + D+GE++
Sbjct: 117 KVVGCSCVAVTDYGEDS 133
>SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein
S12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 148
Score = 150 bits (364), Expect = 8e-38
Identities = 67/107 (62%), Positives = 85/107 (79%)
Frame = +2
Query: 98 MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNE 277
+ V +L+EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E
Sbjct: 30 LSVEDSLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAE 89
Query: 278 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEET 418
Q PLVKV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++
Sbjct: 90 SQTPLVKVADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDS 136
>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 154
Score = 31.9 bits (69), Expect = 0.042
Identities = 17/74 (22%), Positives = 35/74 (47%)
Frame = +2
Query: 104 VNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQ 283
+N + + +K A ++ G+ E KA+ K + L +LA + + LC ++
Sbjct: 37 LNKKMMKTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNN 96
Query: 284 IPLVKVDNNKKLGE 325
+P + + + LGE
Sbjct: 97 VPYLYTVSKELLGE 110
>SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 31.1 bits (67), Expect = 0.073
Identities = 14/54 (25%), Positives = 27/54 (50%)
Frame = +2
Query: 161 HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 322
+GL+ ++ ++A L ++A + D + ALC + +P V N +LG
Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187
>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1129
Score = 25.4 bits (53), Expect = 3.6
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +2
Query: 59 VEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLH 172
+E+ P LS D + + VL L+HGG +HG H
Sbjct: 445 LEIDLEPYLS-ETADKSESHVYVLHGVLVHGGDLHGGH 481
>SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 404
Score = 25.0 bits (52), Expect = 4.8
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = -1
Query: 421 WSFLAEIFDDNTGAADNFPCLAIL 350
W +LA +F N A N P + +L
Sbjct: 258 WEYLASLFTSNFTAVHNIPSVPLL 281
>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
3|||Manual
Length = 923
Score = 25.0 bits (52), Expect = 4.8
Identities = 10/35 (28%), Positives = 19/35 (54%)
Frame = +3
Query: 219 WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 323
++ +V P L+RLC++ R W ++SL+
Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254
>SPAPJ696.01c |vps17||retromer complex subunit
Vps17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 549
Score = 25.0 bits (52), Expect = 4.8
Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%)
Frame = -3
Query: 392 QHRSSRQFSLPCHPCQSCRD-QPILQAF 312
+ ++ +QF LP PCQ+ + +PI+++F
Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 24.6 bits (51), Expect = 6.3
Identities = 15/55 (27%), Positives = 24/55 (43%)
Frame = -3
Query: 248 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 84
C+ S +HIV A L +D +DH G+ L ++P V + P+
Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571
>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
Sec74|Schizosaccharomyces pombe|chr 1|||Manual
Length = 928
Score = 24.6 bits (51), Expect = 6.3
Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -2
Query: 159 TRPPWIKAVFSTSCSATFTSMLLPDKMGLVGTSTSTSA-MVVDYLTVLPRAEF 4
+RP ++F ++ S F+S P L T TST A + L++ P +
Sbjct: 148 SRPSSQSSIFLSNWSTIFSSNASPTDSQLSPTHTSTIAELAASTLSIFPSGSY 200
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 24.6 bits (51), Expect = 6.3
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -3
Query: 155 DHHGLKLSLAPLAVLHSRPC 96
D GL ++ L+++HSRPC
Sbjct: 529 DEMGLGKTIEVLSLIHSRPC 548
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 24.6 bits (51), Expect = 6.3
Identities = 15/55 (27%), Positives = 24/55 (43%)
Frame = -3
Query: 248 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 84
C+ S +HIV A L +D +DH G+ L ++P V + P+
Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 24.2 bits (50), Expect = 8.4
Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
Frame = +2
Query: 140 LIHGGLVHGLHEAAKALDKRQAVLCVLAEN-CDE 238
L+H GL+H L + K +LA N CDE
Sbjct: 896 LLHSGLIHNLLLSLKKFGSSSLRTFLLAMNTCDE 929
>SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 408
Score = 24.2 bits (50), Expect = 8.4
Identities = 8/21 (38%), Positives = 12/21 (57%)
Frame = -3
Query: 71 WGPRLQHQPWLLTI*LSCLVP 9
WGP+ PW+L + S + P
Sbjct: 306 WGPKTAWVPWVLGVIYSLVTP 326
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,811,851
Number of Sequences: 5004
Number of extensions: 34699
Number of successful extensions: 131
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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