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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0250
         (421 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch...   152   2e-38
SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa...   150   8e-38
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce...    32   0.042
SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce...    31   0.073
SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub...    25   3.6  
SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p...    25   4.8  
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch...    25   4.8  
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha...    25   4.8  
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...    25   6.3  
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto...    25   6.3  
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    25   6.3  
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...    25   6.3  
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    24   8.4  
SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces pombe...    24   8.4  

>SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 145

 Score =  152 bits (368), Expect = 2e-38
 Identities = 73/137 (53%), Positives = 97/137 (70%)
 Frame = +2

Query: 8   SARGKTVK*STTMADVEVEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLHEAAKA 187
           S +G  ++    + +VEVE      +S     V  AL+EVLK AL+H GL  G+ EA+KA
Sbjct: 2   SEQGDQIEQVDVVEEVEVEAAAPETVS-----VEDALKEVLKRALVHDGLARGIREASKA 56

Query: 188 LDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKAR 367
           LD+RQA LCVL E+CD+ AY KLV+ALC E + PL+KV + K LGEWAGLC +D+DG AR
Sbjct: 57  LDRRQAHLCVLCESCDQEAYVKLVEALCAESETPLIKVADPKVLGEWAGLCVLDRDGNAR 116

Query: 368 KIVGCSCVVIKDFGEET 418
           K+VGCSCV + D+GE++
Sbjct: 117 KVVGCSCVAVTDYGEDS 133


>SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 148

 Score =  150 bits (364), Expect = 8e-38
 Identities = 67/107 (62%), Positives = 85/107 (79%)
 Frame = +2

Query: 98  MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNE 277
           + V  +L+EVLK AL+H GL  G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E
Sbjct: 30  LSVEDSLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAE 89

Query: 278 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEET 418
            Q PLVKV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++
Sbjct: 90  SQTPLVKVADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDS 136


>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 154

 Score = 31.9 bits (69), Expect = 0.042
 Identities = 17/74 (22%), Positives = 35/74 (47%)
 Frame = +2

Query: 104 VNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQ 283
           +N  + + +K A     ++ G+ E  KA+ K +  L +LA +         +  LC ++ 
Sbjct: 37  LNKKMMKTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNN 96

Query: 284 IPLVKVDNNKKLGE 325
           +P +   + + LGE
Sbjct: 97  VPYLYTVSKELLGE 110


>SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 31.1 bits (67), Expect = 0.073
 Identities = 14/54 (25%), Positives = 27/54 (50%)
 Frame = +2

Query: 161 HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 322
           +GL+     ++ ++A L ++A + D       + ALC +  +P   V N  +LG
Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187


>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
           Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1129

 Score = 25.4 bits (53), Expect = 3.6
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +2

Query: 59  VEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLH 172
           +E+   P LS    D + +   VL   L+HGG +HG H
Sbjct: 445 LEIDLEPYLS-ETADKSESHVYVLHGVLVHGGDLHGGH 481


>SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 404

 Score = 25.0 bits (52), Expect = 4.8
 Identities = 9/24 (37%), Positives = 13/24 (54%)
 Frame = -1

Query: 421 WSFLAEIFDDNTGAADNFPCLAIL 350
           W +LA +F  N  A  N P + +L
Sbjct: 258 WEYLASLFTSNFTAVHNIPSVPLL 281


>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 923

 Score = 25.0 bits (52), Expect = 4.8
 Identities = 10/35 (28%), Positives = 19/35 (54%)
 Frame = +3

Query: 219 WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 323
           ++ +V  P    L+RLC++  R  W     ++SL+
Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254


>SPAPJ696.01c |vps17||retromer complex subunit
           Vps17|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 549

 Score = 25.0 bits (52), Expect = 4.8
 Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%)
 Frame = -3

Query: 392 QHRSSRQFSLPCHPCQSCRD-QPILQAF 312
           + ++ +QF LP  PCQ+  + +PI+++F
Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253


>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 15/55 (27%), Positives = 24/55 (43%)
 Frame = -3

Query: 248 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 84
           C+    S   +HIV  A    L    +D  +DH G+ L ++P  V +      P+
Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571


>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
           Sec74|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 928

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
 Frame = -2

Query: 159 TRPPWIKAVFSTSCSATFTSMLLPDKMGLVGTSTSTSA-MVVDYLTVLPRAEF 4
           +RP    ++F ++ S  F+S   P    L  T TST A +    L++ P   +
Sbjct: 148 SRPSSQSSIFLSNWSTIFSSNASPTDSQLSPTHTSTIAELAASTLSIFPSGSY 200


>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -3

Query: 155 DHHGLKLSLAPLAVLHSRPC 96
           D  GL  ++  L+++HSRPC
Sbjct: 529 DEMGLGKTIEVLSLIHSRPC 548


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 15/55 (27%), Positives = 24/55 (43%)
 Frame = -3

Query: 248 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 84
           C+    S   +HIV  A    L    +D  +DH G+ L ++P  V +      P+
Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
           E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
 Frame = +2

Query: 140 LIHGGLVHGLHEAAKALDKRQAVLCVLAEN-CDE 238
           L+H GL+H L  + K          +LA N CDE
Sbjct: 896 LLHSGLIHNLLLSLKKFGSSSLRTFLLAMNTCDE 929


>SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 408

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 8/21 (38%), Positives = 12/21 (57%)
 Frame = -3

Query: 71  WGPRLQHQPWLLTI*LSCLVP 9
           WGP+    PW+L +  S + P
Sbjct: 306 WGPKTAWVPWVLGVIYSLVTP 326


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,811,851
Number of Sequences: 5004
Number of extensions: 34699
Number of successful extensions: 131
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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