Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0224
         (714 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase |Sc...   178   7e-46
SPBC336.02 |||18S rRNA dimethylase|Schizosaccharomyces pombe|chr...    28   1.2  
SPCC162.05 |coq3||hexaprenyldihydroxybenzoate methyltransferase|...    26   4.7  
SPAPB17E12.10c |||SAM-dependent methyltransferase|Schizosaccharo...    26   4.7  
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...    26   6.1  
SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|ch...    26   6.1  
SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom...    26   6.1  

>SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 230

 Score =  178 bits (433), Expect = 7e-46
 Identities = 88/190 (46%), Positives = 121/190 (63%), Gaps = 6/190 (3%)
 Frame = +1

Query: 145 MAWRSHGANNVDLIRNLRTNGIIKSDTVANAMLAVDRKNYCPSSPYQDSPQSIGFSATIS 324
           M W  + ++N  L+++L  +  + +     AM A  R  YCP SPY DSPQSIG+  TIS
Sbjct: 1   MFWSFNLSSNAALVQHLVESKFLTNQRAIKAMNATSRSFYCPLSPYMDSPQSIGYGVTIS 60

Query: 325 APHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLAT 504
           APHMHA AL++L+  L PG  ALD+GSGSGYL A MA M+   G V GIEHI +LV  + 
Sbjct: 61  APHMHATALQELEPVLQPGCSALDIGSGSGYLVAAMARMVAPNGTVKGIEHIPQLVETSK 120

Query: 505 KNIQND------NPSLLSSERIKLVVGDGRLGYPSEAPYSAIHVGAAAPTLPQALIDQLK 666
           KN+  D         +   +R+++ VGDGR+G   +  + AIHVGA+A  LPQ L+DQLK
Sbjct: 121 KNLLKDINHDEVLMEMYKEKRLQINVGDGRMGTSEDEKFDAIHVGASASELPQKLVDQLK 180

Query: 667 PGGRLIVPVG 696
             G++++P+G
Sbjct: 181 SPGKILIPIG 190


>SPBC336.02 |||18S rRNA dimethylase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 307

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 20/70 (28%), Positives = 35/70 (50%)
 Frame = +1

Query: 361 KNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQNDNPSLLS 540
           K  L   +  L+VG G+G LT     ML +  +V+ +E    +    TK +Q   P    
Sbjct: 45  KADLKQSDTVLEVGPGTGNLT---VRMLEKARKVIAVEMDPRMAAEITKRVQG-TP---K 97

Query: 541 SERIKLVVGD 570
            +++++V+GD
Sbjct: 98  EKKLQVVLGD 107


>SPCC162.05 |coq3||hexaprenyldihydroxybenzoate
           methyltransferase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 271

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 28/109 (25%), Positives = 51/109 (46%), Gaps = 6/109 (5%)
 Frame = +1

Query: 379 GEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQNDNPSLLSSERIKL 558
           G+K LD+G G G L+  MA  LG +  V  ++     + +A K+   D P L  + R++ 
Sbjct: 78  GKKILDIGCGGGILSESMA-RLGAS--VTAVDASPMAIEVAKKHASLD-PVL--NGRLEY 131

Query: 559 VVGDGRLGYPSEAPYSAIHVGAAAPTLPQ------ALIDQLKPGGRLIV 687
           + G    G      +  +        + Q      +L++++KP GRL++
Sbjct: 132 IHGSVE-GSQLPTTFDVVTCMEVLEHVEQPRDFLFSLMEKVKPNGRLVL 179


>SPAPB17E12.10c |||SAM-dependent
           methyltransferase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 301

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 20/73 (27%), Positives = 37/73 (50%), Gaps = 3/73 (4%)
 Frame = +1

Query: 271 SSPYQDSPQSIGFSATISAPHMHAHALEKL--KNQLV-PGEKALDVGSGSGYLTACMAMM 441
           S+P + + ++   S   SA   +   +EK+  ++QL  PG+  +D+G   G  +   A  
Sbjct: 37  SNPPKKTKKNTLISLRKSAETANEKFIEKINKEHQLFKPGQIVVDLGCAPGIWSTIAARH 96

Query: 442 LGETGRVVGIEHI 480
           +G  GRV+  + I
Sbjct: 97  VGLFGRVIACDII 109


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 3/60 (5%)
 Frame = +1

Query: 295 QSIGFSATISAPHMHAHALEKLKNQLVPGEKALD---VGSGSGYLTACMAMMLGETGRVV 465
           QS G S  I A  +H   +    N    G + +    +G   G  T+C+A+M G+T +V+
Sbjct: 17  QSKGASFKIGAS-LHGSRMTARWNSNASGNEKVKGPVIGIDLGTTTSCLAIMEGQTPKVI 75


>SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 862

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 16/51 (31%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
 Frame = +2

Query: 476 IYQNL*IWQPKTSKMTTQAYSHLRGLNL*LVMVVSVIH-LKPLTVQFMSGL 625
           +Y NL   +P +  +  Q Y HL G          V+  + P+ VQFM G+
Sbjct: 423 LYMNLAFVEPSSKLLEDQTYLHLFGSIYNSFREERVMFWIFPIAVQFMRGI 473


>SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1325

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 16/47 (34%), Positives = 20/47 (42%)
 Frame = -2

Query: 521  SFWMFLVAKFTSSDICSIPTTRPVSPNIMAIQAVR*PDPEPTSKAFS 381
            SF   LV K +  D+       P S     I + + P PEPT  A S
Sbjct: 862  SFNKPLVEKESKQDVSDTSDRSPFSFKAFGIDSKKSPTPEPTEMAES 908


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,905,493
Number of Sequences: 5004
Number of extensions: 59635
Number of successful extensions: 162
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)