Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0224
(714 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g48330.2 68416.m05275 protein-L-isoaspartate O-methyltransfer... 205 3e-53
At3g48330.1 68416.m05274 protein-L-isoaspartate O-methyltransfer... 205 3e-53
At5g50240.1 68418.m06222 protein-L-isoaspartate O-methyltransfer... 120 7e-28
At5g14600.1 68418.m01712 expressed protein 35 0.062
At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family prot... 32 0.33
At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se... 32 0.43
At5g64150.1 68418.m08055 methylase family protein contains TIGRf... 31 0.76
At1g17145.1 68414.m02090 expressed protein 31 0.76
At5g52490.1 68418.m06512 fibrillarin, putative similar to fibril... 31 1.0
At5g15540.1 68418.m01819 expressed protein low similarity to DNA... 31 1.0
At1g73320.1 68414.m08485 expressed protein 30 1.3
At3g61990.1 68416.m06962 O-methyltransferase family 3 protein se... 29 2.3
At5g54400.1 68418.m06775 expressed protein 29 3.1
At3g25890.1 68416.m03226 AP2 domain-containing transcription fac... 29 3.1
At3g16260.1 68416.m02051 metallo-beta-lactamase family protein 29 3.1
At3g15530.2 68416.m01969 expressed protein 29 3.1
At3g15530.1 68416.m01968 expressed protein 29 3.1
At5g49020.2 68418.m06066 protein arginine N-methyltransferase fa... 29 4.0
At5g49020.1 68418.m06065 protein arginine N-methyltransferase fa... 29 4.0
At4g26220.1 68417.m03775 caffeoyl-CoA 3-O-methyltransferase, put... 28 5.3
At1g24735.1 68414.m03105 caffeoyl-CoA 3-O-methyltransferase, put... 28 7.1
>At3g48330.2 68416.m05275 protein-L-isoaspartate O-methyltransferase
/ PIMT (PCM) identical to SP|Q42539
Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)
(Protein- beta-aspartate methyltransferase) (PIMT)
(Protein L-isoaspartyl methyltransferase) (L-isoaspartyl
protein carboxyl methyltransferase) {Arabidopsis
thaliana}
Length = 230
Score = 205 bits (500), Expect = 3e-53
Identities = 104/186 (55%), Positives = 129/186 (69%), Gaps = 4/186 (2%)
Frame = +1
Query: 151 WRSHGAN-NVDLIRNLRTNGIIKSDTVANAMLAVDRKNYCP--SSPYQDSPQSIGFSATI 321
W N N ++ NL+ +GI+ SD VA AM AVDR + SS Y DSP SIG++ TI
Sbjct: 5 WSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYNVTI 64
Query: 322 SAPHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLA 501
SAPHMHA L+ L+ L PG + LDVGSG+GYLTAC A+M+G GR +G+EHI ELV +
Sbjct: 65 SAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELVASS 124
Query: 502 TKNIQNDNPSLLSSER-IKLVVGDGRLGYPSEAPYSAIHVGAAAPTLPQALIDQLKPGGR 678
KNI+ S ER + + VGDGR G+ APY AIHVGAAAP +P+ALIDQLKPGGR
Sbjct: 125 VKNIEASAASPFLKERSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKPGGR 184
Query: 679 LIVPVG 696
L++PVG
Sbjct: 185 LVIPVG 190
>At3g48330.1 68416.m05274 protein-L-isoaspartate O-methyltransferase
/ PIMT (PCM) identical to SP|Q42539
Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)
(Protein- beta-aspartate methyltransferase) (PIMT)
(Protein L-isoaspartyl methyltransferase) (L-isoaspartyl
protein carboxyl methyltransferase) {Arabidopsis
thaliana}
Length = 230
Score = 205 bits (500), Expect = 3e-53
Identities = 104/186 (55%), Positives = 129/186 (69%), Gaps = 4/186 (2%)
Frame = +1
Query: 151 WRSHGAN-NVDLIRNLRTNGIIKSDTVANAMLAVDRKNYCP--SSPYQDSPQSIGFSATI 321
W N N ++ NL+ +GI+ SD VA AM AVDR + SS Y DSP SIG++ TI
Sbjct: 5 WSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYNVTI 64
Query: 322 SAPHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLA 501
SAPHMHA L+ L+ L PG + LDVGSG+GYLTAC A+M+G GR +G+EHI ELV +
Sbjct: 65 SAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELVASS 124
Query: 502 TKNIQNDNPSLLSSER-IKLVVGDGRLGYPSEAPYSAIHVGAAAPTLPQALIDQLKPGGR 678
KNI+ S ER + + VGDGR G+ APY AIHVGAAAP +P+ALIDQLKPGGR
Sbjct: 125 VKNIEASAASPFLKERSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKPGGR 184
Query: 679 LIVPVG 696
L++PVG
Sbjct: 185 LVIPVG 190
>At5g50240.1 68418.m06222 protein-L-isoaspartate
O-methyltransferase, putative / PIMT, putative similar
to SP|Q42539 Protein-L-isoaspartate O-methyltransferase
(EC 2.1.1.77) (Protein- beta-aspartate
methyltransferase) (PIMT) (Protein L-isoaspartyl
methyltransferase) (L-isoaspartyl protein carboxyl
methyltransferase) {Arabidopsis thaliana}; contains Pfam
profile PF01135: Protein-L-isoaspartate(D-aspartate)
O-methyltransferase (PCMT)
Length = 269
Score = 120 bits (290), Expect = 7e-28
Identities = 55/98 (56%), Positives = 72/98 (73%), Gaps = 1/98 (1%)
Frame = +1
Query: 406 GSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQND-NPSLLSSERIKLVVGDGRLG 582
G+GYLT C A+M+G GRVVG++HI ELV+++ KNI+ S L + L VGDGR G
Sbjct: 132 GTGYLTGCFALMVGAEGRVVGVDHIPELVDMSIKNIEKSVAASFLKKGSLSLHVGDGRKG 191
Query: 583 YPSEAPYSAIHVGAAAPTLPQALIDQLKPGGRLIVPVG 696
+ APY AIHVGAAA +PQ L+DQLKPGGR+++P+G
Sbjct: 192 WQEFAPYDAIHVGAAASEIPQPLLDQLKPGGRMVIPLG 229
>At5g14600.1 68418.m01712 expressed protein
Length = 318
Score = 34.7 bits (76), Expect = 0.062
Identities = 18/69 (26%), Positives = 35/69 (50%)
Frame = +1
Query: 367 QLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQNDNPSLLSSE 546
++VPG L+ G+GSG L+ +A + TG V + + A ++ + S L +
Sbjct: 105 EVVPGCVVLESGTGSGSLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTV 164
Query: 547 RIKLVVGDG 573
++ + G+G
Sbjct: 165 EVRDIQGEG 173
>At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 198
Score = 32.3 bits (70), Expect = 0.33
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = +1
Query: 379 GEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLA 501
G+ LD+ GSG L ++ +G TG+V+G++ SE + +A
Sbjct: 11 GDYVLDLCCGSGDLAFLLSEKVGSTGKVMGLDFSSEQLAVA 51
>At3g62000.1 68416.m06963 O-methyltransferase family 3 protein
several O-methyltransferases - different species;
contains Pfam 01596 O-methyltransferase domain
Length = 278
Score = 31.9 bits (69), Expect = 0.43
Identities = 14/47 (29%), Positives = 29/47 (61%)
Frame = +1
Query: 367 QLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATK 507
Q++ E+ ++VG +GY + +A++L E+G +V E S + +A +
Sbjct: 115 QMLAAERCIEVGVYTGYSSLAVALVLPESGCLVACERDSNSLEVAKR 161
>At5g64150.1 68418.m08055 methylase family protein contains TIGRfam
TIGR00536: modification methylase, HemK family
Length = 377
Score = 31.1 bits (67), Expect = 0.76
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = +1
Query: 394 DVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQ 516
D+G+GSG + +A +LG GRV+ + + +A N+Q
Sbjct: 206 DLGTGSGAIAIGIAKVLGSRGRVIATDLSPVAIAVAGHNVQ 246
>At1g17145.1 68414.m02090 expressed protein
Length = 335
Score = 31.1 bits (67), Expect = 0.76
Identities = 17/41 (41%), Positives = 26/41 (63%)
Frame = +1
Query: 403 SGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQNDN 525
S SG L AC +L E ++VG+ ++ VNLA++N+ DN
Sbjct: 252 SPSGGL-ACAIAVLAERQQMVGVSSSNQNVNLASQNLVPDN 291
>At5g52490.1 68418.m06512 fibrillarin, putative similar to
fibrillarin from {Xenopus laevis} SP|P22232, {Mus
musculus} SP|P35550, {Homo sapiens} SP|P22087
Length = 292
Score = 30.7 bits (66), Expect = 1.0
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +1
Query: 376 PGEKALDVGSGSGYLTACMAMMLGETGRVVGIEH 477
PG K L +G+ SGY + ++ ++G G V +EH
Sbjct: 133 PGVKVLYLGASSGYTVSHVSDIVGPEGCVYAVEH 166
>At5g15540.1 68418.m01819 expressed protein low similarity to DNA
repair and meiosis protein Rad9 [Coprinus cinereus]
GI:1353390, SP|Q09725 Sister chromatid cohesion protein
mis4 {Schizosaccharomyces pombe}
Length = 1755
Score = 30.7 bits (66), Expect = 1.0
Identities = 24/91 (26%), Positives = 44/91 (48%), Gaps = 5/91 (5%)
Frame = +1
Query: 301 IGFSATISA---PHMHAHALEKLKNQLVPGEKAL--DVGSGSGYLTACMAMMLGETGRVV 465
IG + T+ + P++ +L PGE L +VG GSGY + + +L ++ R+
Sbjct: 20 IGLANTVQSEVTPYLPLPSLPIFCGAAEPGEFKLFDEVGQGSGYRSLDRSEILAQSSRIA 79
Query: 466 GIEHISELVNLATKNIQNDNPSLLSSERIKL 558
+ H +++ L +N + P S E +L
Sbjct: 80 NMLHETDVSYLDLRN-EARAPDCNSGEHFQL 109
>At1g73320.1 68414.m08485 expressed protein
Length = 292
Score = 30.3 bits (65), Expect = 1.3
Identities = 18/48 (37%), Positives = 29/48 (60%)
Frame = +1
Query: 379 GEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQND 522
G+K +++GSG G L C+A +LG G V + + + + L KNIQ +
Sbjct: 122 GKKIVELGSGCG-LVGCIAALLG--GNAV-LTDLPDRLRLLKKNIQTN 165
>At3g61990.1 68416.m06962 O-methyltransferase family 3 protein
several O-methyltransferases - different species;
contains Pfam 01596 O-methyltransferase domain
Length = 290
Score = 29.5 bits (63), Expect = 2.3
Identities = 11/47 (23%), Positives = 30/47 (63%)
Frame = +1
Query: 367 QLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATK 507
+++ ++ ++VG +GY + +A++L E+GR+V + + + +A +
Sbjct: 127 EILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACDKDANALEVAKR 173
>At5g54400.1 68418.m06775 expressed protein
Length = 292
Score = 29.1 bits (62), Expect = 3.1
Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 5/48 (10%)
Frame = +1
Query: 388 ALDVGSGSGYLTACMAMML---GETGRVVGIEH--ISELVNLATKNIQ 516
ALD+G G G L +A L G +GRVVG++ + L L T +I+
Sbjct: 118 ALDLGCGRGILLNAVATQLKKTGSSGRVVGLDRSMTTTLSTLRTAHIE 165
>At3g25890.1 68416.m03226 AP2 domain-containing transcription
factor, putative
Length = 332
Score = 29.1 bits (62), Expect = 3.1
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +3
Query: 117 SSYCNNSNNNGMEKPRSK*C 176
SSYC SNNNG+ K + C
Sbjct: 89 SSYCQESNNNGVSKTKISAC 108
>At3g16260.1 68416.m02051 metallo-beta-lactamase family protein
Length = 937
Score = 29.1 bits (62), Expect = 3.1
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 2/73 (2%)
Frame = +3
Query: 234 CNASC*SEKLLSFFSIPGLSSIYWILCNNQRSPHACSCIRKVKKPASTRRKSFRCWFGVW 413
C +E+LLS IP + + Y L N+ +CI + + T ++R W +
Sbjct: 358 CPTESHAEELLS---IPSMKTYYSCLDNSTDGAKLVNCIIHLSPASVTNSSTYRSWMKRF 414
Query: 414 --LPHCLYGHDVR 446
H L GH+ +
Sbjct: 415 HSAQHILAGHEAK 427
>At3g15530.2 68416.m01969 expressed protein
Length = 288
Score = 29.1 bits (62), Expect = 3.1
Identities = 18/42 (42%), Positives = 25/42 (59%), Gaps = 3/42 (7%)
Frame = +1
Query: 388 ALDVGSGSGYLTACMAMML---GETGRVVGIEHISELVNLAT 504
ALD+G G G L +A L G +GRVVG++ S+ L+T
Sbjct: 115 ALDLGCGRGILLNAVATQLKKTGSSGRVVGLDR-SKRTTLST 155
>At3g15530.1 68416.m01968 expressed protein
Length = 288
Score = 29.1 bits (62), Expect = 3.1
Identities = 18/42 (42%), Positives = 25/42 (59%), Gaps = 3/42 (7%)
Frame = +1
Query: 388 ALDVGSGSGYLTACMAMML---GETGRVVGIEHISELVNLAT 504
ALD+G G G L +A L G +GRVVG++ S+ L+T
Sbjct: 115 ALDLGCGRGILLNAVATQLKKTGSSGRVVGLDR-SKRTTLST 155
>At5g49020.2 68418.m06066 protein arginine N-methyltransferase
family protein similar to protein arginine
methyltransferase [Mus musculus] GI:5257221
Length = 526
Score = 28.7 bits (61), Expect = 4.0
Identities = 27/77 (35%), Positives = 37/77 (48%)
Frame = +1
Query: 337 HAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQ 516
HA +E + G +DVG+GSG L+ M L V +E SE+ A K I
Sbjct: 173 HAAVMENRSD--FSGRVVVDVGAGSGILS--MFAALAGAKHVYAVE-ASEMAEYARKLIA 227
Query: 517 NDNPSLLSSERIKLVVG 567
NP L +ERI ++ G
Sbjct: 228 G-NP--LLAERITVIKG 241
>At5g49020.1 68418.m06065 protein arginine N-methyltransferase
family protein similar to protein arginine
methyltransferase [Mus musculus] GI:5257221
Length = 528
Score = 28.7 bits (61), Expect = 4.0
Identities = 27/77 (35%), Positives = 37/77 (48%)
Frame = +1
Query: 337 HAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQ 516
HA +E + G +DVG+GSG L+ M L V +E SE+ A K I
Sbjct: 175 HAAVMENRSD--FSGRVVVDVGAGSGILS--MFAALAGAKHVYAVE-ASEMAEYARKLIA 229
Query: 517 NDNPSLLSSERIKLVVG 567
NP L +ERI ++ G
Sbjct: 230 G-NP--LLAERITVIKG 243
>At4g26220.1 68417.m03775 caffeoyl-CoA 3-O-methyltransferase,
putative similar to caffeoyl-CoA O-methyltransferase
[GI:1622926][Nicotiana tabacum], GI:2960356 [Populus
balsamifera subsp. trichocarpa], AF036095 [Pinus taeda]
Length = 232
Score = 28.3 bits (60), Expect = 5.3
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +1
Query: 370 LVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIE 474
LV K ++VG +GY A+ L E G+V+ I+
Sbjct: 63 LVNARKTIEVGVFTGYSLLLTALTLPEDGKVIAID 97
>At1g24735.1 68414.m03105 caffeoyl-CoA 3-O-methyltransferase,
putative similar to SP|Q43237 [Vitis vinifera],
GI:684942 [Medicago sativa subsp. sativa]
Length = 195
Score = 27.9 bits (59), Expect = 7.1
Identities = 17/67 (25%), Positives = 31/67 (46%)
Frame = +1
Query: 319 ISAPHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNL 498
+ P H L L +++ +K +++G +GY A+ L G V GI+ E +
Sbjct: 11 MEVPVDEGHFLSMLL-KIMNAKKTIELGVFTGYSLLTTALALPHDGHVTGIDIDKEAYEM 69
Query: 499 ATKNIQN 519
+ I+N
Sbjct: 70 GLEFIKN 76
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,569,313
Number of Sequences: 28952
Number of extensions: 327548
Number of successful extensions: 823
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 796
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 818
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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