BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0212 (610 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 79 2e-15 At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 78 4e-15 At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 78 4e-15 At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 77 1e-14 At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 76 2e-14 At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 76 2e-14 At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 73 1e-13 At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 71 8e-13 At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 70 1e-12 At1g80460.1 68414.m09423 glycerol kinase, putative similar to gl... 33 0.15 At3g28650.1 68416.m03576 DC1 domain-containing protein similar ... 28 5.6 At1g08050.1 68414.m00879 zinc finger (C3HC4-type RING finger) fa... 28 5.6 At2g20960.1 68415.m02479 expressed protein pEARLI 4 gene product... 27 7.4 >At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180 GI:2970034 from [Arabidopsis thaliana] Length = 305 Score = 79.0 bits (186), Expect = 2e-15 Identities = 32/105 (30%), Positives = 56/105 (53%), Gaps = 3/105 (2%) Frame = +3 Query: 288 SALYGLYLCFSSA---KWATIIMAYVILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMV 458 ++L+ +LC + W + +A ++ +G+T HR HR++K + L+ Sbjct: 44 ASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAY 103 Query: 459 MNSFAFQNSAITWIRDHRMHHRYSDTDADPHNATRGFFYSHIGWL 593 A Q I W+ HR HH+++D+D DPH+ GF++SH+ WL Sbjct: 104 CGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWL 148 >At1g06360.1 68414.m00672 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 299 Score = 78.2 bits (184), Expect = 4e-15 Identities = 29/89 (32%), Positives = 49/89 (55%) Frame = +3 Query: 327 KWATIIMAYVILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRD 506 KW + +V+ + +T HR HR++K + L+ L FA Q + W+ Sbjct: 54 KWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDWVSI 113 Query: 507 HRMHHRYSDTDADPHNATRGFFYSHIGWL 593 HR HH+++D+D DPH+ GF++SH+ W+ Sbjct: 114 HRFHHQFTDSDRDPHSPIEGFWFSHVWWI 142 >At1g06090.1 68414.m00638 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase GB:BAA25180 GI:2970034 (ADS1) from [Arabidopsis thaliana] Length = 299 Score = 78.2 bits (184), Expect = 4e-15 Identities = 29/89 (32%), Positives = 49/89 (55%) Frame = +3 Query: 327 KWATIIMAYVILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRD 506 KW + ++ I + +T HR TH+++K + L+ FA Q I W+ Sbjct: 54 KWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDWVST 113 Query: 507 HRMHHRYSDTDADPHNATRGFFYSHIGWL 593 HR HH+++D+D DPH+ GF++SH+ W+ Sbjct: 114 HRFHHQFTDSDRDPHSPIEGFWFSHVFWI 142 >At1g06120.1 68414.m00641 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase GB:BAA25180 GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported by cDNA:gi_12083275_gb_AF332434.1_AF332434 Length = 299 Score = 76.6 bits (180), Expect = 1e-14 Identities = 30/88 (34%), Positives = 49/88 (55%) Frame = +3 Query: 330 WATIIMAYVILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRDH 509 W A ++ I+ + +T HR THR++K + L+ FA Q I W+ H Sbjct: 55 WEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTH 114 Query: 510 RMHHRYSDTDADPHNATRGFFYSHIGWL 593 R HH+++D+D DPH+ GF++SH+ W+ Sbjct: 115 RFHHQFTDSDRDPHSPIEGFWFSHVFWI 142 >At3g15850.1 68416.m02005 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 371 Score = 75.8 bits (178), Expect = 2e-14 Identities = 28/89 (31%), Positives = 53/89 (59%), Gaps = 1/89 (1%) Frame = +3 Query: 330 WATIIMAY-VILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRD 506 W + +A+ + ++ +G+T HR +H+A+K + L+ + + A Q + I W+ Sbjct: 125 WRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVST 184 Query: 507 HRMHHRYSDTDADPHNATRGFFYSHIGWL 593 HR HH++ D+D DPH+ GF++SH+ W+ Sbjct: 185 HRYHHQFCDSDRDPHSPLDGFWFSHMNWM 213 >At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from [Arabidopsis thaliana] Length = 307 Score = 75.8 bits (178), Expect = 2e-14 Identities = 27/88 (30%), Positives = 50/88 (56%) Frame = +3 Query: 330 WATIIMAYVILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRDH 509 W+ + + ++ +G+T HR HR++K + L+ +L A Q I W+ H Sbjct: 63 WSALWVTFLFYTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTH 122 Query: 510 RMHHRYSDTDADPHNATRGFFYSHIGWL 593 R HH+++D++ DPH+ GF++SH+ W+ Sbjct: 123 RYHHQFTDSERDPHSPKEGFWFSHLLWI 150 >At1g06100.1 68414.m00639 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 299 Score = 72.9 bits (171), Expect = 1e-13 Identities = 28/89 (31%), Positives = 47/89 (52%) Frame = +3 Query: 327 KWATIIMAYVILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRD 506 KW ++ I + +T HR THR++K + L+ A Q + W+ Sbjct: 54 KWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSI 113 Query: 507 HRMHHRYSDTDADPHNATRGFFYSHIGWL 593 HR HH+++D+D DPH+ GF++SH+ W+ Sbjct: 114 HRFHHQFTDSDRDPHSPIEGFWFSHVLWI 142 >At1g06350.1 68414.m00671 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 300 Score = 70.5 bits (165), Expect = 8e-13 Identities = 28/89 (31%), Positives = 46/89 (51%) Frame = +3 Query: 327 KWATIIMAYVILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRD 506 KW + V+ + +T HR +HR++K + L+ FA Q + W+ Sbjct: 55 KWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDWVSI 114 Query: 507 HRMHHRYSDTDADPHNATRGFFYSHIGWL 593 HR HH+++D+D DPH+ G +SHI W+ Sbjct: 115 HRFHHQFTDSDRDPHSPKEGLLFSHILWI 143 >At3g15870.1 68416.m02007 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 292 Score = 69.7 bits (163), Expect = 1e-12 Identities = 25/71 (35%), Positives = 43/71 (60%) Frame = +3 Query: 381 VTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRDHRMHHRYSDTDADPHNAT 560 +T HR +HR++ + L+ + AFQ I W+ +HR HH++ +T DPH+ T Sbjct: 66 ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPT 125 Query: 561 RGFFYSHIGWL 593 +GF++SH+ W+ Sbjct: 126 QGFWFSHMAWI 136 >At1g80460.1 68414.m09423 glycerol kinase, putative similar to glycerol kinase (ATP:glycerol 3-phosphotransferase, Glycerokinase, GK)[Mycobacterium tuberculosis] Swiss-Prot:O69664 Length = 522 Score = 33.1 bits (72), Expect = 0.15 Identities = 18/53 (33%), Positives = 27/53 (50%) Frame = +3 Query: 348 AYVILIAAEVGVTAGAHRLWTHRAYKAKRPLQIILMVMNSFAFQNSAITWIRD 506 A+++L EV + +G H L T AYK Q + S A +A+ W+RD Sbjct: 278 AFILLNTGEVPIKSG-HGLLTTLAYKLGPQAQTNYALEGSIAIAGAAVQWLRD 329 >At3g28650.1 68416.m03576 DC1 domain-containing protein similar to hypothetical protein GI:4204272 from [Arabidopsis thaliana] contains weak PHD zinc finger motifs contains weak PHD zinc finger motifs DC1 domain, a divergent protein kinase C domain of unknown function. Length = 665 Score = 27.9 bits (59), Expect = 5.6 Identities = 11/35 (31%), Positives = 18/35 (51%) Frame = -3 Query: 410 GPESMGACRHSDLCRYQYDVGHNYRRPFRTTEAKI 306 G E+ H D+C + D+G R P R + +K+ Sbjct: 201 GVETANLLYHCDMCMFNLDIGCAIRNPERVSLSKL 235 >At1g08050.1 68414.m00879 zinc finger (C3HC4-type RING finger) family protein low similarity to SP|P79263 Inter-alpha-trypsin inhibitor heavy chain H4 precursor {Sus scrofa}; contains Pfam profiles PF00097: Zinc finger, C3HC4 type (RING finger), PF00092: von Willebrand factor type A domain Length = 641 Score = 27.9 bits (59), Expect = 5.6 Identities = 15/40 (37%), Positives = 17/40 (42%) Frame = +2 Query: 446 HTNGNELVRLPKLGHHLDPGPSDAPQIQRHGRRPSQRYQR 565 H N NE + LPK P PSDA + RP R Sbjct: 13 HLNLNECLTLPKTLDPSSPSPSDASRSSVDPNRPDSSSSR 52 >At2g20960.1 68415.m02479 expressed protein pEARLI 4 gene product [Arabidopsis thaliana] GI:871782 Length = 748 Score = 27.5 bits (58), Expect = 7.4 Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (3%) Frame = +2 Query: 422 QSQEATADHTNGNELVRL-PKLGHHLDP 502 +S ++ DHTNGN L RL P G ++P Sbjct: 82 ESSPSSYDHTNGNFLSRLSPLQGRKIEP 109 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,881,632 Number of Sequences: 28952 Number of extensions: 263326 Number of successful extensions: 639 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 630 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 639 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1216725696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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