BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0195 (669 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g20360.1 68415.m02377 expressed protein 67 1e-11 At3g23660.1 68416.m02975 transport protein, putative similar to ... 29 2.1 At1g32220.1 68414.m03963 expressed protein 29 2.1 At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein s... 29 3.7 At5g59540.1 68418.m07461 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 4.9 At2g42650.1 68415.m05278 60S ribosomal protein-related similar t... 28 4.9 At3g56330.1 68416.m06262 N2,N2-dimethylguanosine tRNA methyltran... 27 8.5 >At2g20360.1 68415.m02377 expressed protein Length = 402 Score = 66.9 bits (156), Expect = 1e-11 Identities = 50/168 (29%), Positives = 82/168 (48%), Gaps = 2/168 (1%) Frame = +3 Query: 36 FKYNDVHVDGVRRIARICREEG-VERFIHLSYLNAEEHPKPLVLKKPSAWKISKYLGECA 212 F + D + ++A + +E G + R+I +S L A + PS +K E A Sbjct: 154 FSFEDANHHIAEKLALVAKEHGGIMRYIQVSCLGAS-------VSSPSRMLRAKAAAEEA 206 Query: 213 VREEYPTATIIRASDIYGSEDRFLRSLVNKMRSHSNLMPLYKNGLATVKQPVFVSDVAQG 392 V P ATI+R + + G+EDR L ++ + +PL G T QPV+V DVA Sbjct: 207 VLNALPEATIMRPATMIGTEDRILNPWSMFVKKY-GFLPLIGGG-TTKFQPVYVVDVAAA 264 Query: 393 IVNAARDDDTKC-EVYQAVGPKRYLLADLVDWFYKLMRKDEKWGGYIR 533 IV A +DD + + Y+ GP + +L + Y ++R +W Y++ Sbjct: 265 IVAALKDDGSSMGKTYELGGPDVFTTHELAEIMYDMIR---EWPRYVK 309 >At3g23660.1 68416.m02975 transport protein, putative similar to Swiss-Prot:Q15436 protein transport protein Sec23A [Homo sapiens] Length = 765 Score = 29.5 bits (63), Expect = 2.1 Identities = 17/58 (29%), Positives = 31/58 (53%) Frame = +3 Query: 213 VREEYPTATIIRASDIYGSEDRFLRSLVNKMRSHSNLMPLYKNGLATVKQPVFVSDVA 386 VRE +P ++ D +GS+ RFL + +N +++N N ++T +F DV+ Sbjct: 699 VRERFPVPRLV-VCDQHGSQARFLLAKLNPSATYNN-----ANEMSTGSDVIFTDDVS 750 >At1g32220.1 68414.m03963 expressed protein Length = 296 Score = 29.5 bits (63), Expect = 2.1 Identities = 20/54 (37%), Positives = 27/54 (50%) Frame = +3 Query: 258 IYGSEDRFLRSLVNKMRSHSNLMPLYKNGLATVKQPVFVSDVAQGIVNAARDDD 419 IY S +RF+R L + S L P PV V D+A ++NA +DDD Sbjct: 235 IYDSAERFIRPLRSLPASDLILAP-----------PVNVDDLALAVINAVKDDD 277 >At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein similar to GI:5579331 from [Homo sapiens]; contains Pfam profile PF01532: Glycosyl hydrolase family 47 Length = 624 Score = 28.7 bits (61), Expect = 3.7 Identities = 9/20 (45%), Positives = 13/20 (65%) Frame = +3 Query: 507 DEKWGGYIRYDMKYDPILPL 566 D W GY +Y M YD ++P+ Sbjct: 137 DHAWSGYRKYAMGYDELMPI 156 >At5g59540.1 68418.m07461 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to desacetoxyvindoline-4-hydroxylase [Catharanthus roseus] GI:2352812; contains Pfam profile PF03171: oxidoreductase, 2OG-Fe(II) oxygenase family Length = 366 Score = 28.3 bits (60), Expect = 4.9 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -2 Query: 113 ESLNSFFSADSGNSSYTINVNIIVFESS 30 E SFFS D+GN + N N ++ SS Sbjct: 121 EVKKSFFSRDAGNKKFVYNSNFDLYSSS 148 >At2g42650.1 68415.m05278 60S ribosomal protein-related similar to PBK1 protein (GI:3668141) [Homo sapiens]; weak similarity to 60S ribosomal protein L10a. (Swiss-Prot:Q9SW75) [Chlamydomonas reinhardtii] Length = 372 Score = 28.3 bits (60), Expect = 4.9 Identities = 28/84 (33%), Positives = 40/84 (47%), Gaps = 2/84 (2%) Frame = +3 Query: 174 SAWKISKYLGECAVREEYPTATIIRASDIYGSEDRFLRSLVNKMRSHSNLMPLYKN--GL 347 SA K++K E E TAT+ D+ S +++RSL K+ S S +PLY+ L Sbjct: 187 SAIKVAKLSMESDDIVENVTATLNGVVDVLPSRWKYIRSLHLKL-SESLSLPLYQTVPYL 245 Query: 348 ATVKQPVFVSDVAQGIVNAARDDD 419 P+ V +V G A D D Sbjct: 246 QLKIDPLGVEEVKNGEGLAKSDVD 269 >At3g56330.1 68416.m06262 N2,N2-dimethylguanosine tRNA methyltransferase family protein low similarity to SP|Q9P804 N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) {Schizosaccharomyces pombe}; contains Pfam profile PF02005: N2,N2-dimethylguanosine tRNA methyltransferase Length = 433 Score = 27.5 bits (58), Expect = 8.5 Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 2/45 (4%) Frame = +3 Query: 180 WKISKYLGECAVREEYPTATIIRASDI--YGSEDRFLRSLVNKMR 308 W ++ L A+ E Y A DI +GS+ FLR N +R Sbjct: 145 WVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALR 189 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,517,778 Number of Sequences: 28952 Number of extensions: 297460 Number of successful extensions: 768 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 750 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 767 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1412971776 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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