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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0195
         (669 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g20360.1 68415.m02377 expressed protein                             67   1e-11
At3g23660.1 68416.m02975 transport protein, putative similar to ...    29   2.1  
At1g32220.1 68414.m03963 expressed protein                             29   2.1  
At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein s...    29   3.7  
At5g59540.1 68418.m07461 oxidoreductase, 2OG-Fe(II) oxygenase fa...    28   4.9  
At2g42650.1 68415.m05278 60S ribosomal protein-related similar t...    28   4.9  
At3g56330.1 68416.m06262 N2,N2-dimethylguanosine tRNA methyltran...    27   8.5  

>At2g20360.1 68415.m02377 expressed protein
          Length = 402

 Score = 66.9 bits (156), Expect = 1e-11
 Identities = 50/168 (29%), Positives = 82/168 (48%), Gaps = 2/168 (1%)
 Frame = +3

Query: 36  FKYNDVHVDGVRRIARICREEG-VERFIHLSYLNAEEHPKPLVLKKPSAWKISKYLGECA 212
           F + D +     ++A + +E G + R+I +S L A        +  PS    +K   E A
Sbjct: 154 FSFEDANHHIAEKLALVAKEHGGIMRYIQVSCLGAS-------VSSPSRMLRAKAAAEEA 206

Query: 213 VREEYPTATIIRASDIYGSEDRFLRSLVNKMRSHSNLMPLYKNGLATVKQPVFVSDVAQG 392
           V    P ATI+R + + G+EDR L      ++ +   +PL   G  T  QPV+V DVA  
Sbjct: 207 VLNALPEATIMRPATMIGTEDRILNPWSMFVKKY-GFLPLIGGG-TTKFQPVYVVDVAAA 264

Query: 393 IVNAARDDDTKC-EVYQAVGPKRYLLADLVDWFYKLMRKDEKWGGYIR 533
           IV A +DD +   + Y+  GP  +   +L +  Y ++R   +W  Y++
Sbjct: 265 IVAALKDDGSSMGKTYELGGPDVFTTHELAEIMYDMIR---EWPRYVK 309


>At3g23660.1 68416.m02975 transport protein, putative similar to
           Swiss-Prot:Q15436 protein transport protein Sec23A [Homo
           sapiens]
          Length = 765

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 17/58 (29%), Positives = 31/58 (53%)
 Frame = +3

Query: 213 VREEYPTATIIRASDIYGSEDRFLRSLVNKMRSHSNLMPLYKNGLATVKQPVFVSDVA 386
           VRE +P   ++   D +GS+ RFL + +N   +++N      N ++T    +F  DV+
Sbjct: 699 VRERFPVPRLV-VCDQHGSQARFLLAKLNPSATYNN-----ANEMSTGSDVIFTDDVS 750


>At1g32220.1 68414.m03963 expressed protein
          Length = 296

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 20/54 (37%), Positives = 27/54 (50%)
 Frame = +3

Query: 258 IYGSEDRFLRSLVNKMRSHSNLMPLYKNGLATVKQPVFVSDVAQGIVNAARDDD 419
           IY S +RF+R L +   S   L P           PV V D+A  ++NA +DDD
Sbjct: 235 IYDSAERFIRPLRSLPASDLILAP-----------PVNVDDLALAVINAVKDDD 277


>At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein
           similar to GI:5579331 from [Homo sapiens]; contains Pfam
           profile PF01532: Glycosyl hydrolase family 47
          Length = 624

 Score = 28.7 bits (61), Expect = 3.7
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +3

Query: 507 DEKWGGYIRYDMKYDPILPL 566
           D  W GY +Y M YD ++P+
Sbjct: 137 DHAWSGYRKYAMGYDELMPI 156


>At5g59540.1 68418.m07461 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein similar to
           desacetoxyvindoline-4-hydroxylase [Catharanthus roseus]
           GI:2352812; contains Pfam profile PF03171:
           oxidoreductase, 2OG-Fe(II) oxygenase family
          Length = 366

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = -2

Query: 113 ESLNSFFSADSGNSSYTINVNIIVFESS 30
           E   SFFS D+GN  +  N N  ++ SS
Sbjct: 121 EVKKSFFSRDAGNKKFVYNSNFDLYSSS 148


>At2g42650.1 68415.m05278 60S ribosomal protein-related similar to
           PBK1 protein (GI:3668141) [Homo sapiens]; weak
           similarity to 60S ribosomal protein L10a.
           (Swiss-Prot:Q9SW75) [Chlamydomonas reinhardtii]
          Length = 372

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 28/84 (33%), Positives = 40/84 (47%), Gaps = 2/84 (2%)
 Frame = +3

Query: 174 SAWKISKYLGECAVREEYPTATIIRASDIYGSEDRFLRSLVNKMRSHSNLMPLYKN--GL 347
           SA K++K   E     E  TAT+    D+  S  +++RSL  K+ S S  +PLY+    L
Sbjct: 187 SAIKVAKLSMESDDIVENVTATLNGVVDVLPSRWKYIRSLHLKL-SESLSLPLYQTVPYL 245

Query: 348 ATVKQPVFVSDVAQGIVNAARDDD 419
                P+ V +V  G   A  D D
Sbjct: 246 QLKIDPLGVEEVKNGEGLAKSDVD 269


>At3g56330.1 68416.m06262 N2,N2-dimethylguanosine tRNA
           methyltransferase family protein low similarity to
           SP|Q9P804 N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
           {Schizosaccharomyces pombe}; contains Pfam profile
           PF02005: N2,N2-dimethylguanosine tRNA methyltransferase
          Length = 433

 Score = 27.5 bits (58), Expect = 8.5
 Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 2/45 (4%)
 Frame = +3

Query: 180 WKISKYLGECAVREEYPTATIIRASDI--YGSEDRFLRSLVNKMR 308
           W ++  L   A+ E Y  A      DI  +GS+  FLR   N +R
Sbjct: 145 WVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALR 189


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,517,778
Number of Sequences: 28952
Number of extensions: 297460
Number of successful extensions: 768
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 750
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 767
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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