BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0157 (511 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_28840| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.1 SB_46293| Best HMM Match : Keratin_B2 (HMM E-Value=0.00077) 27 6.8 SB_3680| Best HMM Match : Keratin_B2 (HMM E-Value=0.0024) 27 6.8 >SB_28840| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 615 Score = 27.9 bits (59), Expect = 5.1 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +3 Query: 228 FKPKRITASRQK*AGGGTYPRGLTRGPTTSNYANYNLADFI 350 FK + + A + GGG+YP L R P+ ++ Y+L F+ Sbjct: 529 FKTRAVEAVFYRVGGGGSYPTPLNRVPSLADC--YDLVPFV 567 >SB_46293| Best HMM Match : Keratin_B2 (HMM E-Value=0.00077) Length = 677 Score = 27.5 bits (58), Expect = 6.8 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = +2 Query: 197 SQYSYNDCPTLQTETHYCFTAEIGRWWYLPART 295 S+ N C +T+ ++C T++I WY P +T Sbjct: 185 SKTQANWCHPYKTQANWCHTSKIQSNWYHPNKT 217 Score = 27.1 bits (57), Expect = 9.0 Identities = 9/28 (32%), Positives = 15/28 (53%) Frame = +2 Query: 230 QTETHYCFTAEIGRWWYLPARTHKRSYH 313 +T+ + C +I WY P++T YH Sbjct: 90 ETQAYLCHPTKIQANWYHPSKTQANLYH 117 Score = 27.1 bits (57), Expect = 9.0 Identities = 9/28 (32%), Positives = 15/28 (53%) Frame = +2 Query: 230 QTETHYCFTAEIGRWWYLPARTHKRSYH 313 +T+ + C +I WY P++T YH Sbjct: 622 ETQAYLCHPTKIQANWYHPSKTQANLYH 649 >SB_3680| Best HMM Match : Keratin_B2 (HMM E-Value=0.0024) Length = 180 Score = 27.5 bits (58), Expect = 6.8 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = +2 Query: 197 SQYSYNDCPTLQTETHYCFTAEIGRWWYLPART 295 S+ N C +T+ ++C T++I WY P +T Sbjct: 30 SKTQANWCHPYKTQANWCHTSKIQSNWYHPNKT 62 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,428,407 Number of Sequences: 59808 Number of extensions: 331300 Number of successful extensions: 538 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 462 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 537 length of database: 16,821,457 effective HSP length: 77 effective length of database: 12,216,241 effective search space used: 1123894172 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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