BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0153 (315 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 29 0.17 SPAC19G12.15c |tpp1||trehalose-6-phosphate phosphatase Tpp1|Schi... 26 1.5 SPBC1289.06c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 2.7 SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosac... 25 3.6 SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase Ark1|Schizosa... 25 3.6 SPBC8E4.01c ||SPBP4G3.01|inorganic phosphate transporter |Schizo... 24 4.7 SPAC5H10.12c |||acetylglucosaminyltransferase|Schizosaccharomyce... 24 4.7 SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomy... 24 4.7 SPAC29A4.19c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 24 4.7 SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13 |Schizo... 23 8.3 SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz... 23 8.3 SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizos... 23 8.3 >SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 29.1 bits (62), Expect = 0.17 Identities = 23/74 (31%), Positives = 36/74 (48%) Frame = +3 Query: 18 QEFGTRIRETQATNMKTIICLFTIAIAAMAAVTNLSNVLKNGNDNFTARMFTEVVKNNPG 197 Q G R+RETQA N K++ CL + I A+ +N S + + + T ++K + G Sbjct: 732 QAVGDRLRETQAIN-KSLSCLGDV-IHALGNASN-STTKEKSHIPYRNSKLTYLLKYSLG 788 Query: 198 KSVVLSAFSVLPPL 239 K F + PL Sbjct: 789 KGAKTLMFVNVSPL 802 >SPAC19G12.15c |tpp1||trehalose-6-phosphate phosphatase Tpp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 817 Score = 25.8 bits (54), Expect = 1.5 Identities = 12/33 (36%), Positives = 14/33 (42%) Frame = +2 Query: 44 DAGHKHEDNHLFVYYRHRGNGSRHKSL*CAQKW 142 D K E +F YY R GS + CA W Sbjct: 653 DMSWKKEVRRIFQYYTDRTQGSSIEEKRCAMTW 685 >SPBC1289.06c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 481 Score = 25.0 bits (52), Expect = 2.7 Identities = 11/22 (50%), Positives = 13/22 (59%) Frame = +3 Query: 117 NLSNVLKNGNDNFTARMFTEVV 182 N N+LK ND AR +TE V Sbjct: 56 NSQNLLKQLNDEMKARKYTETV 77 >SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 24.6 bits (51), Expect = 3.6 Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 3/45 (6%) Frame = -2 Query: 185 FHYFGKHSGCEVIVS---IFEHIREICDGCHCRDGDSKQTNDCLH 60 F FG+H CEV+++ + EI G H D D + LH Sbjct: 59 FWRFGRHKSCEVVLNGPRVSNFHFEIYQG-HRNDSDESENVVFLH 102 >SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase Ark1|Schizosaccharomyces pombe|chr 3|||Manual Length = 355 Score = 24.6 bits (51), Expect = 3.6 Identities = 11/54 (20%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = +2 Query: 17 AGIRHEDS-RDAGHKHEDNHLFVYYRHRGNGSRHKSL*CAQKWKR*LHSQNVYR 175 + +RH++ R GH H++ +++ G G ++ L A+++ + S+ +++ Sbjct: 142 SNLRHKNILRLYGHFHDEKRIYLILEFAGRGELYQHLRRAKRFSEEVASKYIFQ 195 >SPBC8E4.01c ||SPBP4G3.01|inorganic phosphate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 572 Score = 24.2 bits (50), Expect = 4.7 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = +3 Query: 66 TIICLFTIAIAAMAAVTNLSNVLKNGNDNFTARMFTEVVKNNPGKSVVLSA 218 T +C F + IA N S +LKN + + ++KN G ++ A Sbjct: 351 TSVCWFLLDIAFYGVNLNQSVILKNIGFSTGTNEYRTLMKNAIGNLIIAVA 401 >SPAC5H10.12c |||acetylglucosaminyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 371 Score = 24.2 bits (50), Expect = 4.7 Identities = 14/53 (26%), Positives = 24/53 (45%) Frame = +3 Query: 69 IICLFTIAIAAMAAVTNLSNVLKNGNDNFTARMFTEVVKNNPGKSVVLSAFSV 227 II + T+ L++ L + +NF V+K PGK +S F++ Sbjct: 25 IIVMTTLVFNGHDYKQTLNDRLTSLKNNFVEENDNAVLKEEPGKYTYMSLFTM 77 >SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 573 Score = 24.2 bits (50), Expect = 4.7 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = +3 Query: 66 TIICLFTIAIAAMAAVTNLSNVLKNGNDNFTARMFTEVVKNNPGKSVVLSA 218 T +C F + IA N S +LKN + + ++KN G ++ A Sbjct: 352 TSVCWFLLDIAFYGVNLNQSVILKNIGFSSGTNEYRTLMKNAIGNLIIAVA 402 >SPAC29A4.19c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1096 Score = 24.2 bits (50), Expect = 4.7 Identities = 14/51 (27%), Positives = 24/51 (47%) Frame = +3 Query: 78 LFTIAIAAMAAVTNLSNVLKNGNDNFTARMFTEVVKNNPGKSVVLSAFSVL 230 L + +A + VT+ ++NG D FT +V +N S S ++L Sbjct: 145 LMPLETSAYSLVTSTPAYIQNGLDTFTIAKLRQVYGSNSLVSTKKSIVTIL 195 >SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 23.4 bits (48), Expect = 8.3 Identities = 16/54 (29%), Positives = 28/54 (51%) Frame = -2 Query: 308 VREADSFQKLFMGFTIRS*S*LSERRQHRKCREDNAFPWIIFHYFGKHSGCEVI 147 ++EAD +K + ++S + Q K + + F +I+F YF K S +VI Sbjct: 76 MKEADYHRKFKSNYLLKSYT-----HQLVKEADGSEFVYILFPYFAKGSVAQVI 124 >SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 23.4 bits (48), Expect = 8.3 Identities = 10/38 (26%), Positives = 18/38 (47%) Frame = -2 Query: 185 FHYFGKHSGCEVIVSIFEHIREICDGCHCRDGDSKQTN 72 F YF ++ +I+ + E ++ + D C D K N Sbjct: 156 FTYFSENLARHIIIQVAEAVKHLHDVCGIVHRDIKPEN 193 >SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 23.4 bits (48), Expect = 8.3 Identities = 8/10 (80%), Positives = 9/10 (90%) Frame = +3 Query: 126 NVLKNGNDNF 155 N+L NGNDNF Sbjct: 170 NILPNGNDNF 179 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,309,292 Number of Sequences: 5004 Number of extensions: 24262 Number of successful extensions: 77 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 76 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 77 length of database: 2,362,478 effective HSP length: 63 effective length of database: 2,047,226 effective search space used: 83936266 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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