BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0143 (432 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 138 1e-33 At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 138 1e-33 At4g34220.1 68417.m04862 leucine-rich repeat transmembrane prote... 29 1.4 At2g47550.1 68415.m05934 pectinesterase family protein contains ... 28 3.1 At4g19515.1 68417.m02871 disease resistance family protein simil... 27 4.1 At1g64110.2 68414.m07264 AAA-type ATPase family protein contains... 27 4.1 At1g64110.1 68414.m07263 AAA-type ATPase family protein contains... 27 4.1 At5g56890.1 68418.m07099 protein kinase family protein contains ... 27 5.5 At5g47330.1 68418.m05834 palmitoyl protein thioesterase family p... 27 5.5 At3g27150.1 68416.m03396 kelch repeat-containing F-box family pr... 27 5.5 At1g18390.1 68414.m02297 protein kinase family protein contains ... 27 5.5 At3g22960.1 68416.m02895 pyruvate kinase, putative similar to py... 26 9.6 At1g16210.1 68414.m01941 expressed protein ESTs gb|T04357 and gb... 26 9.6 >At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S roibosomal protein L4, Arabidopsis thaliana, EMBL:CAA79104 Length = 407 Score = 138 bits (335), Expect = 1e-33 Identities = 64/128 (50%), Positives = 88/128 (68%), Gaps = 1/128 (0%) Frame = +1 Query: 28 AWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIFNKDQG-LTRAFRNIPGVEXXXXXX 204 A+ D K S +R GKGKMRNRR I RKGPL++F + + +AFRN+PGVE Sbjct: 182 AYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVFGTEGAKIVKAFRNLPGVELCHVER 241 Query: 205 XXXXXXAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLK 384 APGGHLGRFVIWT+SAF +L+ ++GS++ PS++KK + LP+ KM N DL R++ Sbjct: 242 LNLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIIN 301 Query: 385 SDEIRKVL 408 SDE++ V+ Sbjct: 302 SDEVQSVV 309 >At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong similarity to 60S ribosomal protein L1 GB:P49691 Length = 406 Score = 138 bits (334), Expect = 1e-33 Identities = 65/133 (48%), Positives = 89/133 (66%), Gaps = 1/133 (0%) Frame = +1 Query: 28 AWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIFNKDQG-LTRAFRNIPGVEXXXXXX 204 A+ D K S +R GKGKMRNRR I RKGPL+++ + + +AFRN+PGVE Sbjct: 181 AYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVYGTEGSKIVKAFRNLPGVELCHVER 240 Query: 205 XXXXXXAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLK 384 APGGHLGRFVIWT+SAF +L+ ++GS++ PS++KK + LP+ KM N DL R++ Sbjct: 241 LNLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIIN 300 Query: 385 SDEIRKVLRAPNK 423 SDEI+ V+ K Sbjct: 301 SDEIQSVVNPIKK 313 >At4g34220.1 68417.m04862 leucine-rich repeat transmembrane protein kinase, putative protein kinase TMKL1, Arabidopsis thaliana, PID:E353150 Length = 757 Score = 29.1 bits (62), Expect = 1.4 Identities = 22/68 (32%), Positives = 31/68 (45%) Frame = -1 Query: 252 DETSKMTSRSQLQEVQLVNIQELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHFTLTS 73 DETS TS S ++ Q V G + +SQT ++ D E L LDT A + Sbjct: 421 DETS--TSESDVENQQTVQAFTRTDGGQLKQSSQTQLVTVDGETRLDLDTLLKASAYILG 478 Query: 72 TKTLRLVH 49 T +V+ Sbjct: 479 TTGTGIVY 486 >At2g47550.1 68415.m05934 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 560 Score = 27.9 bits (59), Expect = 3.1 Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 2/65 (3%) Frame = -1 Query: 210 VQLVNIQELHTGDVAESASQTLILVED--YEGSLTLDTTTVAHFTLTSTKTLRLVHLKDI 37 + +++ + G VA A T+ +ED + SLT+D + T STKTL L +D+ Sbjct: 78 ISMIDAELDRKGKVA--AKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDV 135 Query: 36 RPCLS 22 LS Sbjct: 136 HTFLS 140 >At4g19515.1 68417.m02871 disease resistance family protein similar to downy mildew resistance protein RPP5 [Arabidopsis thaliana] GI:6449046; contains Pfam profile PF01582: TIR domain Length = 524 Score = 27.5 bits (58), Expect = 4.1 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = -1 Query: 213 EVQLVNIQELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHFTLTSTKTL 61 E+ VN QE+H GD + ++ D GS + +T T ++ S K L Sbjct: 101 EIFPVNKQEMHVGDSCTITKCGVYVINDAAGSSSGNTMTPQCSSMDSLKLL 151 >At1g64110.2 68414.m07264 AAA-type ATPase family protein contains Pfam domain, PF00004: ATPase, AAA family Length = 829 Score = 27.5 bits (58), Expect = 4.1 Identities = 18/77 (23%), Positives = 42/77 (54%), Gaps = 4/77 (5%) Frame = -1 Query: 258 PDDETSKMTSRSQLQ-EVQLVNIQELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHF- 85 P+DET ++ +SQL+ ++ ++ Q+ + + E S+ ++ +D E DT ++++ Sbjct: 337 PEDETHLVSWKSQLERDMNMIQTQD-NRNHIMEVLSENDLICDDLESISFEDTKVLSNYI 395 Query: 84 --TLTSTKTLRLVHLKD 40 + S + L++ KD Sbjct: 396 EEIVVSALSYHLMNNKD 412 >At1g64110.1 68414.m07263 AAA-type ATPase family protein contains Pfam domain, PF00004: ATPase, AAA family Length = 824 Score = 27.5 bits (58), Expect = 4.1 Identities = 18/77 (23%), Positives = 42/77 (54%), Gaps = 4/77 (5%) Frame = -1 Query: 258 PDDETSKMTSRSQLQ-EVQLVNIQELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHF- 85 P+DET ++ +SQL+ ++ ++ Q+ + + E S+ ++ +D E DT ++++ Sbjct: 332 PEDETHLVSWKSQLERDMNMIQTQD-NRNHIMEVLSENDLICDDLESISFEDTKVLSNYI 390 Query: 84 --TLTSTKTLRLVHLKD 40 + S + L++ KD Sbjct: 391 EEIVVSALSYHLMNNKD 407 >At5g56890.1 68418.m07099 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 1113 Score = 27.1 bits (57), Expect = 5.5 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = +2 Query: 266 PHSAGLTPYSGHGRHHQNKRRTSTCPNRRWPTLTSHVFSSLMRSGRSSVLP 418 P S P S H +HHQ +++ + P L H+ S + + S+ P Sbjct: 335 PSSPSPPPLSSHHQHHQERKKIADSPAP--SPLPPHLISPKKSNRKGSMTP 383 >At5g47330.1 68418.m05834 palmitoyl protein thioesterase family protein Length = 314 Score = 27.1 bits (57), Expect = 5.5 Identities = 18/55 (32%), Positives = 27/55 (49%), Gaps = 3/55 (5%) Frame = -1 Query: 162 SASQTLILVEDYEGSLTLDTTTVAHFTLTSTKTLRLVH---LKDIRPCLSLVPNS 7 SA QT + ED+ G TLD F + + +R+V +K + P L P+S Sbjct: 246 SAQQTKLYTEDWIGLKTLDDAGKVKFVSVAGEHIRMVDEDVVKHVVPYLQDQPSS 300 >At3g27150.1 68416.m03396 kelch repeat-containing F-box family protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646 F-box domain Length = 422 Score = 27.1 bits (57), Expect = 5.5 Identities = 13/21 (61%), Positives = 15/21 (71%) Frame = +1 Query: 349 KMANTDLTRLLKSDEIRKVLR 411 K+ N +RLLKSDEI KV R Sbjct: 95 KLLNKGFSRLLKSDEIFKVRR 115 >At1g18390.1 68414.m02297 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 605 Score = 27.1 bits (57), Expect = 5.5 Identities = 20/65 (30%), Positives = 32/65 (49%), Gaps = 3/65 (4%) Frame = -1 Query: 192 QELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHFTLTSTKTLRLVHL---KDIRPCLS 22 QE++ ++A Q L + + SL DT T T+ + L L KD+RPC+S Sbjct: 502 QEINLSNMAVVKIQNHELRDMVDPSLGFDTDTRVRQTVIAVAELAFQCLQSDKDLRPCMS 561 Query: 21 LVPNS 7 V ++ Sbjct: 562 HVQDT 566 >At3g22960.1 68416.m02895 pyruvate kinase, putative similar to pyruvate kinase isozyme A, chloroplast precursor [Ricinus communis] SWISS-PROT:Q43117 Length = 596 Score = 26.2 bits (55), Expect = 9.6 Identities = 17/45 (37%), Positives = 24/45 (53%) Frame = +2 Query: 284 TPYSGHGRHHQNKRRTSTCPNRRWPTLTSHVFSSLMRSGRSSVLP 418 TP+ H H Q R S+ RR+P LT+ ++S+ S SS P Sbjct: 13 TPHLLHLPHSQFNRPLSSISFRRFP-LTTIKYTSIRASSSSSPSP 56 >At1g16210.1 68414.m01941 expressed protein ESTs gb|T04357 and gb|AA595092 come from this gene Length = 234 Score = 26.2 bits (55), Expect = 9.6 Identities = 10/41 (24%), Positives = 20/41 (48%) Frame = +1 Query: 304 KTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKR 426 K P K+ +P PK+ +L R + D++ +A + + Sbjct: 85 KKPDKKANRVTVPVPKVTEAELIRRREEDQVALAKKAEDSK 125 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,211,169 Number of Sequences: 28952 Number of extensions: 178520 Number of successful extensions: 562 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 553 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 560 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 685039728 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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