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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0136
         (682 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein si...    40   0.002
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN...    30   1.2  
At3g24670.1 68416.m03097 pectate lyase family protein similar to...    29   3.8  
At3g61420.1 68416.m06879 transcription factor-related low simila...    28   5.0  
At1g55750.1 68414.m06383 transcription factor-related low simila...    28   5.0  
At1g28030.1 68414.m03432 oxidoreductase, 2OG-Fe(II) oxygenase fa...    28   5.0  
At3g05740.1 68416.m00644 DNA helicase (RECQI1) identical to DNA ...    28   6.6  
At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PG...    28   6.6  
At2g35110.1 68415.m04307 HEM protein-related weak similarity to ...    28   6.6  
At5g22380.1 68418.m02611 no apical meristem (NAM) family protein...    27   8.7  
At5g01280.1 68418.m00037 expressed protein                             27   8.7  
At3g54920.1 68416.m06086 pectate lyase, putative / powdery milde...    27   8.7  

>At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein similar
            to lysosomal alpha-mannosidase SP:O09159 from [Mus
            musculus]
          Length = 1047

 Score = 39.9 bits (89), Expect = 0.002
 Identities = 26/79 (32%), Positives = 43/79 (54%), Gaps = 6/79 (7%)
 Frame = +2

Query: 104  MPDGLHLLTLEPWG-DQILLRIENYKTGAEDSTI----EIDLQGIFNGIHIKSVKETTLA 268
            +P  + L+TLE      +LLR+ +     EDS      +++L+ +F+G  IK V E +L+
Sbjct: 923  LPQNIALITLEELDLGNVLLRLAHLYEAGEDSDYSKIAKVELKKLFSGKMIKEVTEMSLS 982

Query: 269  ANQ-WLDDYDKWTWNTEGD 322
            ANQ  +   +K  W  EG+
Sbjct: 983  ANQEKVKMKEKMKWKVEGE 1001


>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
            topoisomerase II / DNA gyrase (TOP2) identical to
            SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis
            thaliana}
          Length = 1473

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 20/101 (19%), Positives = 46/101 (45%)
 Frame = +2

Query: 110  DGLHLLTLEPWGDQILLRIENYKTGAEDSTIEIDLQGIFNGIHIKSVKETTLAANQWLDD 289
            +G  ++ ++PW       IE  KT +++      + G++  +   +++ T L   +W DD
Sbjct: 870  NGESMVPMDPWYRGFKGTIE--KTASKEGGCTYTITGLYEEVDETTIRITELPIRRWNDD 927

Query: 290  YDKWTWNTEGDFYESFNKKYGSFEAVNKAVDGPDVREDEGL 412
            Y  +  + + D    F +   ++    K+VD   +  +E +
Sbjct: 928  YKNFLQSLKTDNGAPFFQDVKAYND-EKSVDFDLILSEENM 967


>At3g24670.1 68416.m03097 pectate lyase family protein similar to
           pectate lyase GP:14531296 from [Fragaria x ananassa]
          Length = 440

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 8/24 (33%), Positives = 13/24 (54%)
 Frame = +2

Query: 251 KETTLAANQWLDDYDKWTWNTEGD 322
           KE T     W  ++ +W W ++GD
Sbjct: 366 KEVTKRVGSWQGEWKQWNWRSQGD 389


>At3g61420.1 68416.m06879 transcription factor-related low
           similarity to SP|Q9DBA9 TFIIH basal transcription factor
           complex p62 subunit {Mus musculus}; contains Pfam
           profile PF03909: BSD domain
          Length = 566

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 15/43 (34%), Positives = 21/43 (48%)
 Frame = +2

Query: 311 TEGDFYESFNKKYGSFEAVNKAVDGPDVREDEGLKIKLKAKEI 439
           TE DF+  + +    +   N AV   +  EDE L + LK  EI
Sbjct: 197 TEKDFWTKYFRAEYLYSTKNTAVAAAEAAEDEELAVFLKPDEI 239


>At1g55750.1 68414.m06383 transcription factor-related low
           similarity to SP|Q9DBA9 TFIIH basal transcription factor
           complex p62 subunit {Mus musculus}; contains Pfam
           profile PF03909: BSD domain
          Length = 591

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 15/43 (34%), Positives = 21/43 (48%)
 Frame = +2

Query: 311 TEGDFYESFNKKYGSFEAVNKAVDGPDVREDEGLKIKLKAKEI 439
           TE DF+  + +    +   N AV   +  EDE L + LK  EI
Sbjct: 227 TEKDFWTKYFRAEYLYSTKNTAVAAAEAAEDEELAVFLKPDEI 269


>At1g28030.1 68414.m03432 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein similar to GS-AOP loci [GI:16118889,
           GI:16118887, GI:16118891, GI:16118893]; contains PF03171
           2OG-Fe(II) oxygenase superfamily domain
          Length = 322

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
 Frame = +2

Query: 308 NTEGD--FYESFNKKYGSFEAVNKAVDGPDVREDEGLK-IKLKAKEIRSFI 451
           NTE    FY   ++ + +    N AVDG +++  +G + IK+K  +  SFI
Sbjct: 182 NTENKIGFYPHIDRHFLTILCQNDAVDGLEIKTKDGEEWIKVKPSQASSFI 232


>At3g05740.1 68416.m00644 DNA helicase (RECQI1) identical to DNA
           Helicase [Arabidopsis thaliana] GI:10944747
          Length = 606

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 4/68 (5%)
 Frame = +2

Query: 29  VSDASGLSYEQWMRLENCKKFVKTPMPDGLHLLTLEPWGDQILLRIENYKTGAED----S 196
           +S A+ L +++    +   +FV     DG   +T+E  GD  L  + +++ G E+     
Sbjct: 14  ISLATKLGFDEDSAKKCLDRFVDLYGDDGRDFITVELCGDDFLAALADFEEGTEEWDDIQ 73

Query: 197 TIEIDLQG 220
            IE + QG
Sbjct: 74  AIESEAQG 81


>At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PGP1)
           identical to P-glycoprotein GI:3849833 from [Arabidopsis
           thaliana]; homologous to mammalian mdr gene,contains
           ATP-binding cassette; related to multi drug resistance
           proteins
          Length = 1286

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
 Frame = -1

Query: 427 FKF-DFEAFVLSDVRSVDSFVHGLEAPVFL 341
           F+F D   +VL  + SV +FVHG   P+FL
Sbjct: 33  FRFADGLDYVLMGIGSVGAFVHGCSLPLFL 62


>At2g35110.1 68415.m04307 HEM protein-related weak similarity to
           Membrane-associated protein Hem (Dhem-2)
           (Swiss-Prot:P55162) [Drosophila melanogaster]; weak
           similarity to Nck-associated protein 1 (NAP 1)
           (p125Nap1) (Membrane-associated protein HEM-2)
           (Swiss-Prot:P55161) [Rattus norvegicus]
          Length = 1339

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
 Frame = +2

Query: 65  MRLENCKKFVKTPMPDG--LHLLTLEPWGDQILLRIENYKTGA 187
           ++LE   KF    + DG  LHL +LEPW  Q+LL +  ++  A
Sbjct: 130 LQLERIDKFSLDSLHDGAELHLQSLEPW-IQLLLDLMAFREQA 171


>At5g22380.1 68418.m02611 no apical meristem (NAM) family protein
           contains Pfam PF02365: No apical meristem (NAM) domain;
          Length = 235

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 17/76 (22%), Positives = 30/76 (39%)
 Frame = +3

Query: 132 WSLGATRSC*ESKTTRPVPKIRPSKLTCRAYSTVSISKA*KRQR*PQTSGWTTMISGLGI 311
           W +    +  E+     +PK+R     CR Y T   S+A  R+          + S + +
Sbjct: 130 WKMNEYHAVDETVNASTIPKLRREFSLCRVYITTGSSRAFDRRPEGVLQTERMLTSDVAV 189

Query: 312 RKETFTKASTRNTGAS 359
            + +F   S+  T  S
Sbjct: 190 AETSFRVESSLETSIS 205


>At5g01280.1 68418.m00037 expressed protein
          Length = 460

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 24/81 (29%), Positives = 31/81 (38%), Gaps = 2/81 (2%)
 Frame = +3

Query: 186 PKIRPSKLTCRAYSTVSISKA*KRQR*PQTSGWT--TMISGLGIRKETFTKASTRNTGAS 359
           P  RPS  T RA ST + +          T  W+  +  SG G  + T T A      A+
Sbjct: 107 PAKRPSTPTSRATSTTTRATLTSSSTTSSTRSWSRPSSSSGTGTSRVTLTAAR-----AT 161

Query: 360 RP*TKLSTDLTSERTKASKSN 422
           RP T      T   T    +N
Sbjct: 162 RPTTSTDQQTTGSATSTRSNN 182


>At3g54920.1 68416.m06086 pectate lyase, putative / powdery mildew
           susceptibility protein (PMR6) identical to powdery
           mildew susceptibility protein [Arabidopsis thaliana]
           GI:22506901; similar to pectate lyase 2 GP:6606534 from
           [Musa acuminata]
          Length = 501

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 12/31 (38%), Positives = 16/31 (51%), Gaps = 1/31 (3%)
 Frame = +2

Query: 245 SVKETTLAANQWLD-DYDKWTWNTEGDFYES 334
           S KE T   +   D ++  W W TEGD  E+
Sbjct: 343 SAKEVTKRVDSKDDGEWSNWNWRTEGDLMEN 373


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,689,046
Number of Sequences: 28952
Number of extensions: 313953
Number of successful extensions: 890
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 867
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 890
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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