BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0136 (682 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein si... 40 0.002 At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 30 1.2 At3g24670.1 68416.m03097 pectate lyase family protein similar to... 29 3.8 At3g61420.1 68416.m06879 transcription factor-related low simila... 28 5.0 At1g55750.1 68414.m06383 transcription factor-related low simila... 28 5.0 At1g28030.1 68414.m03432 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 5.0 At3g05740.1 68416.m00644 DNA helicase (RECQI1) identical to DNA ... 28 6.6 At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PG... 28 6.6 At2g35110.1 68415.m04307 HEM protein-related weak similarity to ... 28 6.6 At5g22380.1 68418.m02611 no apical meristem (NAM) family protein... 27 8.7 At5g01280.1 68418.m00037 expressed protein 27 8.7 At3g54920.1 68416.m06086 pectate lyase, putative / powdery milde... 27 8.7 >At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein similar to lysosomal alpha-mannosidase SP:O09159 from [Mus musculus] Length = 1047 Score = 39.9 bits (89), Expect = 0.002 Identities = 26/79 (32%), Positives = 43/79 (54%), Gaps = 6/79 (7%) Frame = +2 Query: 104 MPDGLHLLTLEPWG-DQILLRIENYKTGAEDSTI----EIDLQGIFNGIHIKSVKETTLA 268 +P + L+TLE +LLR+ + EDS +++L+ +F+G IK V E +L+ Sbjct: 923 LPQNIALITLEELDLGNVLLRLAHLYEAGEDSDYSKIAKVELKKLFSGKMIKEVTEMSLS 982 Query: 269 ANQ-WLDDYDKWTWNTEGD 322 ANQ + +K W EG+ Sbjct: 983 ANQEKVKMKEKMKWKVEGE 1001 >At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA topoisomerase II / DNA gyrase (TOP2) identical to SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis thaliana} Length = 1473 Score = 30.3 bits (65), Expect = 1.2 Identities = 20/101 (19%), Positives = 46/101 (45%) Frame = +2 Query: 110 DGLHLLTLEPWGDQILLRIENYKTGAEDSTIEIDLQGIFNGIHIKSVKETTLAANQWLDD 289 +G ++ ++PW IE KT +++ + G++ + +++ T L +W DD Sbjct: 870 NGESMVPMDPWYRGFKGTIE--KTASKEGGCTYTITGLYEEVDETTIRITELPIRRWNDD 927 Query: 290 YDKWTWNTEGDFYESFNKKYGSFEAVNKAVDGPDVREDEGL 412 Y + + + D F + ++ K+VD + +E + Sbjct: 928 YKNFLQSLKTDNGAPFFQDVKAYND-EKSVDFDLILSEENM 967 >At3g24670.1 68416.m03097 pectate lyase family protein similar to pectate lyase GP:14531296 from [Fragaria x ananassa] Length = 440 Score = 28.7 bits (61), Expect = 3.8 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = +2 Query: 251 KETTLAANQWLDDYDKWTWNTEGD 322 KE T W ++ +W W ++GD Sbjct: 366 KEVTKRVGSWQGEWKQWNWRSQGD 389 >At3g61420.1 68416.m06879 transcription factor-related low similarity to SP|Q9DBA9 TFIIH basal transcription factor complex p62 subunit {Mus musculus}; contains Pfam profile PF03909: BSD domain Length = 566 Score = 28.3 bits (60), Expect = 5.0 Identities = 15/43 (34%), Positives = 21/43 (48%) Frame = +2 Query: 311 TEGDFYESFNKKYGSFEAVNKAVDGPDVREDEGLKIKLKAKEI 439 TE DF+ + + + N AV + EDE L + LK EI Sbjct: 197 TEKDFWTKYFRAEYLYSTKNTAVAAAEAAEDEELAVFLKPDEI 239 >At1g55750.1 68414.m06383 transcription factor-related low similarity to SP|Q9DBA9 TFIIH basal transcription factor complex p62 subunit {Mus musculus}; contains Pfam profile PF03909: BSD domain Length = 591 Score = 28.3 bits (60), Expect = 5.0 Identities = 15/43 (34%), Positives = 21/43 (48%) Frame = +2 Query: 311 TEGDFYESFNKKYGSFEAVNKAVDGPDVREDEGLKIKLKAKEI 439 TE DF+ + + + N AV + EDE L + LK EI Sbjct: 227 TEKDFWTKYFRAEYLYSTKNTAVAAAEAAEDEELAVFLKPDEI 269 >At1g28030.1 68414.m03432 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to GS-AOP loci [GI:16118889, GI:16118887, GI:16118891, GI:16118893]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain Length = 322 Score = 28.3 bits (60), Expect = 5.0 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 3/51 (5%) Frame = +2 Query: 308 NTEGD--FYESFNKKYGSFEAVNKAVDGPDVREDEGLK-IKLKAKEIRSFI 451 NTE FY ++ + + N AVDG +++ +G + IK+K + SFI Sbjct: 182 NTENKIGFYPHIDRHFLTILCQNDAVDGLEIKTKDGEEWIKVKPSQASSFI 232 >At3g05740.1 68416.m00644 DNA helicase (RECQI1) identical to DNA Helicase [Arabidopsis thaliana] GI:10944747 Length = 606 Score = 27.9 bits (59), Expect = 6.6 Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 4/68 (5%) Frame = +2 Query: 29 VSDASGLSYEQWMRLENCKKFVKTPMPDGLHLLTLEPWGDQILLRIENYKTGAED----S 196 +S A+ L +++ + +FV DG +T+E GD L + +++ G E+ Sbjct: 14 ISLATKLGFDEDSAKKCLDRFVDLYGDDGRDFITVELCGDDFLAALADFEEGTEEWDDIQ 73 Query: 197 TIEIDLQG 220 IE + QG Sbjct: 74 AIESEAQG 81 >At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PGP1) identical to P-glycoprotein GI:3849833 from [Arabidopsis thaliana]; homologous to mammalian mdr gene,contains ATP-binding cassette; related to multi drug resistance proteins Length = 1286 Score = 27.9 bits (59), Expect = 6.6 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%) Frame = -1 Query: 427 FKF-DFEAFVLSDVRSVDSFVHGLEAPVFL 341 F+F D +VL + SV +FVHG P+FL Sbjct: 33 FRFADGLDYVLMGIGSVGAFVHGCSLPLFL 62 >At2g35110.1 68415.m04307 HEM protein-related weak similarity to Membrane-associated protein Hem (Dhem-2) (Swiss-Prot:P55162) [Drosophila melanogaster]; weak similarity to Nck-associated protein 1 (NAP 1) (p125Nap1) (Membrane-associated protein HEM-2) (Swiss-Prot:P55161) [Rattus norvegicus] Length = 1339 Score = 27.9 bits (59), Expect = 6.6 Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 2/43 (4%) Frame = +2 Query: 65 MRLENCKKFVKTPMPDG--LHLLTLEPWGDQILLRIENYKTGA 187 ++LE KF + DG LHL +LEPW Q+LL + ++ A Sbjct: 130 LQLERIDKFSLDSLHDGAELHLQSLEPW-IQLLLDLMAFREQA 171 >At5g22380.1 68418.m02611 no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain; Length = 235 Score = 27.5 bits (58), Expect = 8.7 Identities = 17/76 (22%), Positives = 30/76 (39%) Frame = +3 Query: 132 WSLGATRSC*ESKTTRPVPKIRPSKLTCRAYSTVSISKA*KRQR*PQTSGWTTMISGLGI 311 W + + E+ +PK+R CR Y T S+A R+ + S + + Sbjct: 130 WKMNEYHAVDETVNASTIPKLRREFSLCRVYITTGSSRAFDRRPEGVLQTERMLTSDVAV 189 Query: 312 RKETFTKASTRNTGAS 359 + +F S+ T S Sbjct: 190 AETSFRVESSLETSIS 205 >At5g01280.1 68418.m00037 expressed protein Length = 460 Score = 27.5 bits (58), Expect = 8.7 Identities = 24/81 (29%), Positives = 31/81 (38%), Gaps = 2/81 (2%) Frame = +3 Query: 186 PKIRPSKLTCRAYSTVSISKA*KRQR*PQTSGWT--TMISGLGIRKETFTKASTRNTGAS 359 P RPS T RA ST + + T W+ + SG G + T T A A+ Sbjct: 107 PAKRPSTPTSRATSTTTRATLTSSSTTSSTRSWSRPSSSSGTGTSRVTLTAAR-----AT 161 Query: 360 RP*TKLSTDLTSERTKASKSN 422 RP T T T +N Sbjct: 162 RPTTSTDQQTTGSATSTRSNN 182 >At3g54920.1 68416.m06086 pectate lyase, putative / powdery mildew susceptibility protein (PMR6) identical to powdery mildew susceptibility protein [Arabidopsis thaliana] GI:22506901; similar to pectate lyase 2 GP:6606534 from [Musa acuminata] Length = 501 Score = 27.5 bits (58), Expect = 8.7 Identities = 12/31 (38%), Positives = 16/31 (51%), Gaps = 1/31 (3%) Frame = +2 Query: 245 SVKETTLAANQWLD-DYDKWTWNTEGDFYES 334 S KE T + D ++ W W TEGD E+ Sbjct: 343 SAKEVTKRVDSKDDGEWSNWNWRTEGDLMEN 373 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,689,046 Number of Sequences: 28952 Number of extensions: 313953 Number of successful extensions: 890 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 867 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 890 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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