BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0090 (722 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosac... 28 1.2 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 8.3 >SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 250 Score = 28.3 bits (60), Expect = 1.2 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = -2 Query: 703 ATRTSSHIQYITTHSTVMTFLYYYIYIRMEHTE 605 A +TS +I +T MTFL + +Y+ E+ E Sbjct: 85 ANKTSENISDFEVRTTEMTFLLFLLYVDEEYEE 117 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.4 bits (53), Expect = 8.3 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +1 Query: 439 NLA*DIFKISLSIYKLRTTTCGPSVYRAIT 528 N+ D+F++ L + L+T CGPS+ R+ T Sbjct: 789 NINNDMFRLRLIVVVLQT--CGPSIIRSKT 816 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,760,135 Number of Sequences: 5004 Number of extensions: 55004 Number of successful extensions: 85 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 84 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 85 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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