BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0084 (532 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 68 1e-12 SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 65 7e-12 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 31 0.081 SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomy... 28 1.00 SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 27 1.3 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 26 4.0 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 26 4.0 SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 25 7.0 SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr 1|... 25 7.0 SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces po... 25 7.0 SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 25 9.3 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 67.7 bits (158), Expect = 1e-12 Identities = 39/124 (31%), Positives = 64/124 (51%), Gaps = 1/124 (0%) Frame = +2 Query: 20 TQSHNMSLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLI 199 T+ N L + R + + + EA++WIE LG P FE L++G VL L+ Sbjct: 20 TKGSNTRLAAKQRETLQAYDYLCRVDEAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLV 79 Query: 200 NKLKPGSVPKINTTGG-QFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVST 376 K +P + KI + QF+ +NI F I G+P+I F+ D++E K++ +V+ Sbjct: 80 QKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYC 139 Query: 377 LFAL 388 + AL Sbjct: 140 IHAL 143 >SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein Stg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 64.9 bits (151), Expect = 7e-12 Identities = 47/146 (32%), Positives = 74/146 (50%), Gaps = 2/146 (1%) Frame = +2 Query: 92 EKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENI 271 EKEA+EWIE L K D L+ G +LC++ + ++ + + F MENI Sbjct: 6 EKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANI-RYKESNMPFVQMENI 64 Query: 272 TNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCLGPKPA 448 + F A + VP D+FQT DL+E+++ QV+ ++ + R + LGPK A Sbjct: 65 SAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLA 124 Query: 449 DECKRDFS-DEVLKAGQTVIGLQAGS 523 ++ R FS + + + V LQ GS Sbjct: 125 EKKPRVFSAQQQREFREGVNSLQYGS 150 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 31.5 bits (68), Expect = 0.081 Identities = 28/101 (27%), Positives = 49/101 (48%), Gaps = 4/101 (3%) Frame = +2 Query: 98 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 277 EA++W+E ++ + F D L +G VLCQL K P + ++++ E T Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTV 125 Query: 278 FQSA----IKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 388 + +A + G+ F+T DL + +I +V+ L AL Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166 >SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 278 Score = 27.9 bits (59), Expect = 1.00 Identities = 20/81 (24%), Positives = 35/81 (43%) Frame = +2 Query: 8 EKALTQSHNMSLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVL 187 EK ++ S++ + + ++A K K + E +K P E V+K+ V Sbjct: 165 EKGKSKPSKKSVKAKKKLRLAEKPASNNSKAGKSSQESKKSSK-APAESAAAVIKEDKVS 223 Query: 188 CQLINKLKPGSVPKINTTGGQ 250 + +K KP P N+T Q Sbjct: 224 DRKKSKKKPKKTPVSNSTASQ 244 >SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 992 Score = 27.5 bits (58), Expect = 1.3 Identities = 18/48 (37%), Positives = 22/48 (45%), Gaps = 4/48 (8%) Frame = -2 Query: 216 PGFSLLMSWQR----TVPSLRTSSNSSPGGNLAPRTPSIHSWASFSFS 85 PG L QR T P L TSS++ PGG + + HS S S Sbjct: 210 PGVGTLQQPQRAGSDTFPDLNTSSSNQPGGEPNVASANTHSLEILSSS 257 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 25.8 bits (54), Expect = 4.0 Identities = 31/108 (28%), Positives = 47/108 (43%), Gaps = 16/108 (14%) Frame = +2 Query: 80 NPEKEKEAQEWIEGVL------GAKFPPG-ELFE--DVLKDGTVLCQLINKLKPGSV--- 223 N E+ +E + I VL G++ P E FE D KDG +L +LIN P ++ Sbjct: 111 NEEERREFIKHINSVLAGDPDVGSRVPINTETFEFFDQCKDGLILSKLINDSVPDTIDER 170 Query: 224 ----PKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKD 355 + N FK +EN ++ KA G I D+ E ++ Sbjct: 171 VLNKQRNNKPLDNFKCIENNNVVINSAKAMGGISITNIGAGDILEGRE 218 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 25.8 bits (54), Expect = 4.0 Identities = 16/41 (39%), Positives = 24/41 (58%) Frame = +2 Query: 44 ERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDV 166 ER+ AK A+K EK+K + ++ EG G + PP E+V Sbjct: 665 EREA-AKAATKSEAEKQKPSGKFEEGT-GGRAPPPPPAEEV 703 >SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 25.0 bits (52), Expect = 7.0 Identities = 10/45 (22%), Positives = 20/45 (44%) Frame = +1 Query: 223 PQDQHHRWTVQNDGEHHKLPVCNQSIRCTRHRCVPNRRSMGEKRH 357 P+ +++++ +HHKL + RC P +K+H Sbjct: 206 PRSSTTSYSIRDADKHHKLTTSGVTKMNITERCKPKTTIQTDKKH 250 >SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr 1|||Manual Length = 376 Score = 25.0 bits (52), Expect = 7.0 Identities = 12/39 (30%), Positives = 19/39 (48%) Frame = -1 Query: 460 FAFVSGLGSEAGAAPFRVSVSLTTEGKQCADYLGDVFFL 344 FAFVS EA + F ++ G Q + + +FF+ Sbjct: 51 FAFVSEKSLEAASVKFVATIIAERMGAQFSSFYDKIFFV 89 >SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces pombe|chr 1|||Manual Length = 1159 Score = 25.0 bits (52), Expect = 7.0 Identities = 12/24 (50%), Positives = 17/24 (70%) Frame = -1 Query: 448 SGLGSEAGAAPFRVSVSLTTEGKQ 377 SG G+ AG++PF S +LT E K+ Sbjct: 656 SGFGNTAGSSPF--SFNLTKESKE 677 >SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1173 Score = 24.6 bits (51), Expect = 9.3 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = -3 Query: 509 DRSQFARPSGLHQKSHVCIRQRAWV 435 DR +F RP H+K +RQR+ V Sbjct: 153 DRVEFKRPKNPHEKHFRPLRQRSSV 177 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,380,111 Number of Sequences: 5004 Number of extensions: 52047 Number of successful extensions: 140 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 140 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 218398248 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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