BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0042 (631 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g18270.1 68416.m02324 mandelate racemase/muconate lactonizing... 29 3.4 At4g35870.1 68417.m05094 expressed protein 28 5.9 At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 27 7.8 >At3g18270.1 68416.m02324 mandelate racemase/muconate lactonizing enzyme family protein low similarity to cis,cis-muconate lactonizing enzyme [Burkholderia sp. TH2] GI:23491535; contains Pfam profile: PF01188 Mandelate racemase/muconate lactonizing enzyme, C-terminal domain, PF02746: Mandelate racemase/muconate lactonizing enzyme, N-terminal domain Length = 410 Score = 28.7 bits (61), Expect = 3.4 Identities = 14/48 (29%), Positives = 25/48 (52%) Frame = +3 Query: 177 LSIAIMRFGSKGGVAVVAILRSWNLYLEVGGAFTL*MSMGSSNHLTPG 320 ++I + + G + V+ + RS + L +GG ++MG S HL G Sbjct: 313 VNIKLAKTGILESLEVIELARSSGIELMIGGMVETRLAMGFSGHLAAG 360 >At4g35870.1 68417.m05094 expressed protein Length = 817 Score = 27.9 bits (59), Expect = 5.9 Identities = 23/88 (26%), Positives = 37/88 (42%) Frame = -3 Query: 587 LKCFFITFVTIIRPLSLCIFELSSVGRR*VRINASFCYIAMIFSFELSSA*KCIDLVRNI 408 L + +F ++ P+ F G R + +F Y+ + F + K +D V I Sbjct: 679 LTMIYSSFAPLVVPVGAVYF-----GYRYIVDKYNFLYVYRVRGFPAGNEGKLMDTVLCI 733 Query: 407 KRFCRELVITLNFFILFFYCLGGWTSPQ 324 RFC +L + +LFF G T Q Sbjct: 734 MRFCVDLYLV--SMLLFFSVKGDSTKLQ 759 >At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1; identical to gi:1703477 Length = 1077 Score = 27.5 bits (58), Expect = 7.8 Identities = 11/33 (33%), Positives = 21/33 (63%) Frame = +3 Query: 42 DNLSSIAAFIVQNKFSDDLEQDLMSRKIYIYSR 140 D + S+A F N + ++++DL SR++ +Y R Sbjct: 52 DTVMSMAEFGNDNSNNQEIDEDLHSRQLAVYGR 84 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,068,539 Number of Sequences: 28952 Number of extensions: 268671 Number of successful extensions: 562 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 548 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 562 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1285411824 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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