BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0034 (696 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 67 3e-12 SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 56 4e-09 SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 52 8e-08 SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 51 1e-07 SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 51 1e-07 SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 43 4e-05 SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Sch... 41 1e-04 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 29 0.84 SPBC56F2.06 |mug147||sequence orphan|Schizosaccharomyces pombe|c... 27 1.9 SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 27 3.4 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 26 4.5 >SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 66.9 bits (156), Expect = 3e-12 Identities = 42/158 (26%), Positives = 76/158 (48%), Gaps = 2/158 (1%) Frame = +1 Query: 4 PTILAKVTPADKVMEDEIFGPILPIVCIENAYEAIKFINEREHPLVLYVFSNQYNIQKLF 183 PT+L T K+ ++E F PI + ++ A++ N E L VF + + F Sbjct: 358 PTLLVDATNEMKIAQEECFAPIALVFRAKSPEHALEIANGTEFGLGASVFGRDKQLCQYF 417 Query: 184 TEQTRSGSISINDTIMFYGVETLPFGGVGNSGIGAYHGKKTIDTFTHKKSCLKKNFAAIG 363 T+ +G +++ND FY ++ +PFGG SG G + G + + + K+ F+AI Sbjct: 418 TDNLETGMVAVNDFGAFYLLQ-MPFGGCKKSGYGRFAGYEGLRGICNSKAIAYDRFSAIH 476 Query: 364 EKLASG-RYP-PYTEGNLKFITALMKKRHGPSLKYLPH 471 + YP P ++ +F+ LM +G + +P+ Sbjct: 477 TGIPPAVDYPIPDSQKAWQFVRGLMGTVYGAWISLVPN 514 >SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 503 Score = 56.4 bits (130), Expect = 4e-09 Identities = 34/111 (30%), Positives = 55/111 (49%) Frame = +1 Query: 1 EPTILAKVTPADKVMEDEIFGPILPIVCIENAYEAIKFINEREHPLVLYVFSNQYNIQKL 180 EPTIL+ VT V ++EIFGP+L ++ + EAI+ N + L V +N Sbjct: 384 EPTILSNVTEDMAVGKEEIFGPVLAVIKFKTIEEAIRRGNNSTYGLAAGVHTNNITNAIK 443 Query: 181 FTEQTRSGSISINDTIMFYGVETLPFGGVGNSGIGAYHGKKTIDTFTHKKS 333 + +G++ +N + + +PFGG SGIG G + +T K+ Sbjct: 444 VSNALEAGTVWVNCYNLLH--HQIPFGGYKESGIGRELGSYGLTNYTQTKA 492 >SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 493 Score = 52.0 bits (119), Expect = 8e-08 Identities = 30/106 (28%), Positives = 50/106 (47%) Frame = +1 Query: 1 EPTILAKVTPADKVMEDEIFGPILPIVCIENAYEAIKFINEREHPLVLYVFSNQYNIQKL 180 EPT+L+ VT V +E FGP+ + ++ E I++ N+ + L YVF+N + Sbjct: 379 EPTVLSGVTQDMLVASEETFGPLASVFKFDDTEEVIEWANDSDVGLAGYVFTNNLSTMIH 438 Query: 181 FTEQTRSGSISINDTIMFYGVETLPFGGVGNSGIGAYHGKKTIDTF 318 ++ G + N I + FGG+ SG G G+ + F Sbjct: 439 VAKELEVGLVGAN--IEMVDEPFISFGGIKQSGFGKEAGRLGVQEF 482 >SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 500 Score = 51.2 bits (117), Expect = 1e-07 Identities = 31/96 (32%), Positives = 49/96 (51%), Gaps = 2/96 (2%) Frame = +1 Query: 4 PTILAKVTPADKVMEDEIFGPILPIVCIENAYEAIKFINEREHPLVLYVFSNQYNIQKLF 183 PT+ V +K+ +EIFGP+L + EA++ N+ E+ L VFS + F Sbjct: 387 PTVFTNVQTHNKIWREEIFGPVLAVKTFHTNEEALELANDSEYGLGSGVFSTNPKTLEFF 446 Query: 184 TEQTRSGSISINDTIMFYGVET--LPFGGVGNSGIG 285 + +G S+N+ Y V T LP+ G +SG+G Sbjct: 447 SNNIEAGMCSLNN----YHVVTHELPWIGWKHSGLG 478 >SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 496 Score = 51.2 bits (117), Expect = 1e-07 Identities = 32/110 (29%), Positives = 54/110 (49%) Frame = +1 Query: 4 PTILAKVTPADKVMEDEIFGPILPIVCIENAYEAIKFINEREHPLVLYVFSNQYNIQKLF 183 PTI A + ++++EIFGP++ I + EAI+ N + L F+ Sbjct: 380 PTIFADCSENMTIVKEEIFGPVVAISKFKTEDEAIEKANNTTYGLAAMCFTKDLERAHRV 439 Query: 184 TEQTRSGSISINDTIMFYGVETLPFGGVGNSGIGAYHGKKTIDTFTHKKS 333 +++ +G + IN T ++ PFGG+ SGIG G I+ +T K+ Sbjct: 440 SDELEAGMVFINST-ENSDIQA-PFGGIKMSGIGNELGSNGIEMYTQIKA 487 >SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 43.2 bits (97), Expect = 4e-05 Identities = 29/111 (26%), Positives = 47/111 (42%) Frame = +1 Query: 1 EPTILAKVTPADKVMEDEIFGPILPIVCIENAYEAIKFINEREHPLVLYVFSNQYNIQKL 180 EPT++ + E+E FGP+ + + E + + N+ L Y+FS + Sbjct: 384 EPTVIINAKQGMLISEEETFGPVGALFKFDTEDEVVAWANDSPVGLAGYLFSKDISRVFR 443 Query: 181 FTEQTRSGSISINDTIMFYGVETLPFGGVGNSGIGAYHGKKTIDTFTHKKS 333 E + G + N ++ + PFGGV SG G K I + KS Sbjct: 444 VGEALQVGMVGCNTGLVSDVLS--PFGGVKESGFGREGSKYGISEYLDIKS 492 >SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 41.1 bits (92), Expect = 1e-04 Identities = 35/124 (28%), Positives = 56/124 (45%), Gaps = 6/124 (4%) Frame = +1 Query: 1 EPTILAKVTPADKVMEDEIFGPILPIVCIE-NAYEAIKFINEREHP--LVLYVFSNQYNI 171 EPT+L P + +E+FGP+L + E + +A+ + + P L +F+ + Sbjct: 415 EPTVLLSKNPKHDIFVNELFGPVLSVYVYEDDNLDAVCDLIDTTTPYGLTGSIFAQDRVV 474 Query: 172 QKLFTEQTR--SGSISINDTIMFYGVETLPFGGVGNSGIGAYHGKKTI-DTFTHKKSCLK 342 + T++ R +G+ IND V PFGG SG G I F +S +K Sbjct: 475 VRKLTDRLRNAAGNFYINDKCTGAVVGEQPFGGARASGTNDKAGSGMILSRFVSPRS-IK 533 Query: 343 KNFA 354 FA Sbjct: 534 DTFA 537 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 28.7 bits (61), Expect = 0.84 Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 3/30 (10%) Frame = +1 Query: 298 KKTIDTFTHKK---SCLKKNFAAIGEKLAS 378 KKT+DTF +K SCLK N I +K+ S Sbjct: 1369 KKTLDTFLMEKLCSSCLKNNIEIIPDKINS 1398 >SPBC56F2.06 |mug147||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 411 Score = 27.5 bits (58), Expect = 1.9 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +3 Query: 3 AHNIGQGHAGRQSDGRRDIRSHLTNSLY 86 AH+I + G+ + + + SHLTN LY Sbjct: 61 AHSISKPFDGQNDEEKPSLYSHLTNELY 88 >SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1234 Score = 26.6 bits (56), Expect = 3.4 Identities = 20/76 (26%), Positives = 33/76 (43%) Frame = +1 Query: 382 RYPPYTEGNLKFITALMKKRHGPSLKYLPHFMAFALGAAISYGIFAWQKMASDDS*KHEA 561 R+ GN++ I + + S YL + F+ A +G+ W + SDD +E Sbjct: 484 RFQSTVLGNMEDIQSQTEFFSKTSPLYLTMDVGFSTIEAFLHGVTRWNENTSDDPATYEI 543 Query: 562 MTRRATLSWHNQLFYI 609 + + L W QL I Sbjct: 544 ILQNLFL-WCKQLVEI 558 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/38 (28%), Positives = 19/38 (50%) Frame = +1 Query: 298 KKTIDTFTHKKSCLKKNFAAIGEKLASGRYPPYTEGNL 411 K TH+KS + + EK+++ + PY GN+ Sbjct: 54 KSEFQRLTHEKSTMLEKIKENQEKISNNKMLPYLVGNV 91 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,872,381 Number of Sequences: 5004 Number of extensions: 60213 Number of successful extensions: 150 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 145 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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