Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0025
         (713 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    50   3e-07
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo...    48   1e-06
SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo...    39   8e-04
SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyc...    30   0.29 
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch...    29   0.66 
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S...    28   1.2  
SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz...    27   2.7  
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c...    27   3.5  
SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr 1||...    26   4.7  
SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub...    26   6.1  
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo...    26   6.1  
SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pomb...    26   6.1  
SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|ch...    26   6.1  
SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|c...    26   6.1  
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...    25   8.1  
SPCC895.04c |ufe1||SNARE Ufe1|Schizosaccharomyces pombe|chr 3|||...    25   8.1  

>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 50.0 bits (114), Expect = 3e-07
 Identities = 53/210 (25%), Positives = 87/210 (41%), Gaps = 18/210 (8%)
 Frame = +2

Query: 59  VLTLYKMDASPPVRAVYMVIEALNIP-NVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTD 235
           ++ L+ +  S   R V+M +E L +P  +K  D     +      + KL+P    P++ D
Sbjct: 1   MIVLHHLKNSRSTRIVWM-LEELKVPYEIKVYD---RVDGRAPPAYTKLSPLGKSPIVVD 56

Query: 236 DKFVIWDSHAIATYLLNKYGKGSSYYPEDPEKRALIDMRLHFDSGILYP--------ALR 391
           D     +S AI  +L+ KYG       ED  +    ++ +HF    L P         L 
Sbjct: 57  DGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWMHFSEASLMPFIWASHVLDLS 116

Query: 392 ENDEPIFFWGETTFKPQGLAKIKSAY---------DFTEKFLSDSPWIAGDDVTVADMSC 544
            N  PIFF        Q +  IKS Y         D+ +  L+ + + AG+  T AD   
Sbjct: 117 VNMTPIFF---RYIVRQFVNGIKSKYLSKETFLNLDYIDNHLASNEYFAGEQFTAADPQM 173

Query: 545 VATIGSLDALLPINEKEYPKITSWLKRCSE 634
              I +      +++K Y  I  W++  S+
Sbjct: 174 CFPIFAAQRDY-LSQKPYKNIKRWMRVVSD 202


>SPCC965.07c |gst2||glutathione S-transferase
           Gst2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 230

 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 55/207 (26%), Positives = 84/207 (40%), Gaps = 20/207 (9%)
 Frame = +2

Query: 65  TLYKMDASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTDDK- 241
           TLY     P    V + ++ LN+ + + +  D    +   +E L LNP   +P L D K 
Sbjct: 5   TLYSHAGGPNPWKVVLALKELNL-SYEQIFYDFQKGEQKCKEHLALNPNGRVPTLVDHKN 63

Query: 242 --FVIWDSHAIATYLLNKY--GKGSSYYPEDPEKRALIDMRLHFDSGILYPALRENDEPI 409
             + IW+S AI  YL +KY   +  S   +DPE   LI   L F +        +     
Sbjct: 64  NDYTIWESDAILIYLADKYDTDRKISLSFDDPEYYKLIQY-LFFQASGQGVIWGQAGWFN 122

Query: 410 FFWGETTFK--PQGLAKIKSAYDFTEKFLSDSPWIAGDDVTVADMSCVATIGSLDALLPI 583
           FF  E       +   +IK      E  L D  ++  +  T+AD+S +    +L  L   
Sbjct: 123 FFHHEPVVSAVTRYRNEIKRVLGVLEDILKDRDYLVANKYTIADLSFIPWNYNLGGLFGE 182

Query: 584 N-------------EKEYPKITSWLKR 625
                         EKE+PK  +W +R
Sbjct: 183 GKFSFKEEVPQLDFEKEFPKAYAWNQR 209


>SPCC191.09c |gst1||glutathione S-transferase
           Gst1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 229

 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 46/198 (23%), Positives = 81/198 (40%), Gaps = 25/198 (12%)
 Frame = +2

Query: 107 YMVIEALNIPNVKYVD--VDLLAEDHLKEEFLKLNPQHTIPMLTD---DKFVIWDSHAIA 271
           + V++AL   ++ Y    V+    +    E L LNP   +P L D   + + IW+S AI 
Sbjct: 16  WKVVQALKELDLTYETRYVNFSKNEQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAIL 75

Query: 272 TYLLNKYG-KGSSYYPEDPEKRALIDMRLHFDS---GILYPA---LRENDEPIFFWGETT 430
            YL +KY  +     P D  +   +   L F +   GI++          + +     T 
Sbjct: 76  IYLADKYDTERKISLPRDHPEYYKVIQYLFFQASGQGIIWGQAGWFSVYHQELVISAITR 135

Query: 431 FKPQGLAKIKSAYDFTEKFLSDSPWIAGDDVTVADMSCVATIGSLDALLPIN-------- 586
           ++ +    IK      E  L D  ++  +  T+AD+S ++    L+ +            
Sbjct: 136 YRNE----IKRVLGVLEDILKDRDYLVANRFTIADLSFISWNNFLEIIFAEGKFSIEEEV 191

Query: 587 -----EKEYPKITSWLKR 625
                EKE+P+  SW +R
Sbjct: 192 PQLDFEKEFPRTYSWHQR 209


>SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 313

 Score = 30.3 bits (65), Expect = 0.29
 Identities = 36/157 (22%), Positives = 61/157 (38%), Gaps = 16/157 (10%)
 Frame = +2

Query: 203 NPQHTIPMLTDDKFVIWDSHAIATYLLNKYGKGSSYYPEDPEKRAL----IDMRLHFD-- 364
           N + T+P+L D K+    ++  A  ++  +    +   ED EKR +      +R   D  
Sbjct: 113 NMRFTVPVLWDSKYNTIVNNESAE-IIRMFNDAFNEVIEDEEKRVVDLYPSSLRTKIDEL 171

Query: 365 SGILYPALRENDEPIFFWGETTFKPQGLAKIKSAYDFTEKFLSDS--PWIAGDDVTVADM 538
           +   Y  +        F        + +  +    D  E+ L +S  P++ GD +T  D+
Sbjct: 172 NDYFYDTVNNGVYKTGFATTAEAYEKNVRVVFQGLDRLEQVLKESKGPFLLGDHLTETDV 231

Query: 539 SCVATIGSLDALLPINEK--------EYPKITSWLKR 625
               TI   D +   + K         YP I  WLKR
Sbjct: 232 RLYTTIVRFDPVYVQHFKCNIGTIRHNYPHINQWLKR 268


>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 29.1 bits (62), Expect = 0.66
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = +2

Query: 512 GDDVTVADMSCVATIGSLDALLPINEKEYP 601
           G D+ VAD S   T  S DA++ + +K YP
Sbjct: 655 GSDILVADPSTTVTPKSRDAVMDVVQKYYP 684


>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 462

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 12/41 (29%), Positives = 23/41 (56%)
 Frame = +2

Query: 302 SSYYPEDPEKRALIDMRLHFDSGILYPALRENDEPIFFWGE 424
           ++YY E  EK  +  +++ ++   +   LR   EPI ++GE
Sbjct: 29  AAYYAEQKEKDRIKALKIPYEDSKVREYLRRYGEPITYFGE 69


>SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1473

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 14/35 (40%), Positives = 17/35 (48%)
 Frame = +2

Query: 434 KPQGLAKIKSAYDFTEKFLSDSPWIAGDDVTVADM 538
           K    A IKS  D +E F + S WI G D    D+
Sbjct: 501 KKDSSAPIKSLLDRSEFFTNVSHWIIGSDAWAYDL 535


>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 521

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 14/43 (32%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
 Frame = +2

Query: 425 TTFKPQGLAKIKSAYD-FTEKFLSDSPWIAGDDVTVADMSCVA 550
           T F PQG  K  S YD + E+ L     I+     +  + C+A
Sbjct: 324 TRFSPQGFEKFYSNYDYYKERILLRPQVISNHPEIIRRLGCIA 366


>SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 335

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 12/31 (38%), Positives = 16/31 (51%)
 Frame = +2

Query: 557 GSLDALLPINEKEYPKITSWLKRCSELDFYQ 649
           G LD LLP+ +     I S  K   +L FY+
Sbjct: 30  GKLDILLPVEDASEEAIRSITKIVQDLSFYE 60


>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
           Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1129

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 10/36 (27%), Positives = 20/36 (55%)
 Frame = +2

Query: 128 NIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTD 235
           ++PNV  + +     D L++  +K+N +H  P+  D
Sbjct: 413 SLPNVLQLQLKRFDYDMLRDMMVKINDRHEFPLEID 448


>SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1309

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 13/33 (39%), Positives = 19/33 (57%)
 Frame = +2

Query: 392  ENDEPIFFWGETTFKPQGLAKIKSAYDFTEKFL 490
            EN  P+ F+GE    PQG   ++S+  F E F+
Sbjct: 1047 ENILPLHFYGELVKSPQGCEVLESSGHF-ESFM 1078


>SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 510

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 24/83 (28%), Positives = 36/83 (43%), Gaps = 5/83 (6%)
 Frame = +2

Query: 473 FTEKFLSDSPWIAGDDVTVADMSCVAT-IGSLDALLP----INEKEYPKITSWLKRCSEL 637
           F EKFL + P  A D+  +A  S     I  + A L     +N         W+   S  
Sbjct: 153 FFEKFLEEFPERANDEWFIAGESFAGQYIPHIAAKLKEKNLVNLGGLAIGNGWINPLSHY 212

Query: 638 DFYQRGNNVKGLLEFKALLKQYL 706
           + Y      KG+++F++ L QYL
Sbjct: 213 ETYLNYLVEKGMVDFESELGQYL 235


>SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 587

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 2/34 (5%)
 Frame = +2

Query: 290 YGKGSSYY--PEDPEKRALIDMRLHFDSGILYPA 385
           Y   +SY+   + P+ RAL+D   H+D+   YPA
Sbjct: 455 YTTQNSYFLSRDTPKLRALVDALRHYDTKSGYPA 488


>SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 819

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 16/79 (20%), Positives = 33/79 (41%)
 Frame = +2

Query: 155 VDLLAEDHLKEEFLKLNPQHTIPMLTDDKFVIWDSHAIATYLLNKYGKGSSYYPEDPEKR 334
           +D L   +   +FL L   H +P+   +   ++ +HA+   L  K     S+   +P   
Sbjct: 351 LDRLNSLYCNTQFLNLEDIH-VPLPMRETLFMYKAHAVTETLTGKPSSPDSFTNANPTTA 409

Query: 335 ALIDMRLHFDSGILYPALR 391
            ++      + G+L   +R
Sbjct: 410 PIVSRNAQDNMGMLAYMIR 428


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 20/83 (24%), Positives = 35/83 (42%)
 Frame = +1

Query: 238 QIRHLGQSCNSYIFAKQIRQRQFILSRGPREESPDRYEITFRQWNIVSCFARKRRAHFLL 417
           ++ + G    SY+F   I  R    S GP   S   Y  +    ++ S  A +    F +
Sbjct: 18  KLGYAGNDAPSYVFPTVIATRSAGASSGPAVSSKPSYMASKGSGHLSSKRATE-DLDFFI 76

Query: 418 GRDHLQASRSGQNKVSL*FHGEV 486
           G D L+ + +G +      HG++
Sbjct: 77  GNDALKKASAGYSLDYPIRHGQI 99


>SPCC895.04c |ufe1||SNARE Ufe1|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 319

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 10/35 (28%), Positives = 19/35 (54%)
 Frame = +2

Query: 518 DVTVADMSCVATIGSLDALLPINEKEYPKITSWLK 622
           +V+ A  SC+  I  L  ++   + + PK + WL+
Sbjct: 98  EVSSAISSCIHQIAKLQEIVKEQQSQIPKKSGWLQ 132


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,075,369
Number of Sequences: 5004
Number of extensions: 68524
Number of successful extensions: 195
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)