Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0009
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56450.1 68414.m06492 20S proteasome beta subunit G1 (PBG1) (... 132 2e-31
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 36 0.020
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 35 0.060
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 35 0.060
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 35 0.060
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 35 0.060
At5g45430.1 68418.m05582 protein kinase, putative contains simil... 29 3.0
At3g55140.2 68416.m06124 pectate lyase family protein similar to... 29 3.9
At3g55140.1 68416.m06123 pectate lyase family protein similar to... 29 3.9
At3g14470.1 68416.m01833 disease resistance protein (NBS-LRR cla... 29 3.9
At1g07725.1 68414.m00833 exocyst subunit EXO70 family protein co... 28 5.2
At5g04140.2 68418.m00402 glutamate synthase (GLU1) / ferredoxin-... 27 9.0
At5g04140.1 68418.m00401 glutamate synthase (GLU1) / ferredoxin-... 27 9.0
At4g05090.1 68417.m00756 inositol monophosphatase family protein... 27 9.0
At1g01620.1 68414.m00079 plasma membrane intrinsic protein 1C (P... 27 9.0
>At1g56450.1 68414.m06492 20S proteasome beta subunit G1 (PBG1)
(PRCH) identical to 20S proteasome beta subunit (PBG1)
GI:3421123 [Arabidopsis thaliana]; identical to cDNA
proteasome subunit prch GI:2511597
Length = 246
Score = 132 bits (319), Expect = 2e-31
Identities = 55/149 (36%), Positives = 96/149 (64%)
Frame = +1
Query: 208 PITTTTTVIGVKFDKGCVIAGDTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADFQYL 387
P T T+++ +K+ G ++A D GSYGS R+++ RV + LLG G+ +DFQ +
Sbjct: 27 PYVTGTSIVAIKYKDGVLMASDMGGSYGSTLRYKNIERVKAIGKHSLLGASGEISDFQEI 86
Query: 388 KDIIQQKIIDERCVGDGLQLKPRSLHCWLTRVLYNKRSKMDPLWNSYVVAGIQDGEPFLG 567
+ + +++ DG L P+ +H +LTRV+YN+R+K +PLWN+ V+ G+++G+ +LG
Sbjct: 87 LRYLDELTLNDNMWDDGNSLGPKEIHNYLTRVMYNRRNKFNPLWNTLVLGGVKNGKSYLG 146
Query: 568 AVDKLGTAYEDAVISNGLGAYMATPLLRD 654
V +G ++ED ++ G G ++A P+LRD
Sbjct: 147 MVSMIGVSFEDDHVATGFGNHLARPILRD 175
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 36.3 bits (80), Expect = 0.020
Identities = 18/66 (27%), Positives = 34/66 (51%)
Frame = +1
Query: 205 SPITTTTTVIGVKFDKGCVIAGDTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADFQY 384
+P + TT+IGV ++ G V+ D+ S G R ++ ++ D + + G AD Q
Sbjct: 7 APHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQV 66
Query: 385 LKDIIQ 402
+ D ++
Sbjct: 67 VSDYVR 72
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 34.7 bits (76), Expect = 0.060
Identities = 15/67 (22%), Positives = 34/67 (50%)
Frame = +1
Query: 211 ITTTTTVIGVKFDKGCVIAGDTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADFQYLK 390
+ T TT++G+ F G ++ DT + G + ++C ++ + I G AD + +
Sbjct: 36 LKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVT 95
Query: 391 DIIQQKI 411
D++ ++
Sbjct: 96 DMVSSQL 102
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 34.7 bits (76), Expect = 0.060
Identities = 15/67 (22%), Positives = 34/67 (50%)
Frame = +1
Query: 211 ITTTTTVIGVKFDKGCVIAGDTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADFQYLK 390
+ T TT++G+ F G ++ DT + G + ++C ++ + I G AD + +
Sbjct: 36 LKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVT 95
Query: 391 DIIQQKI 411
D++ ++
Sbjct: 96 DMVSSQL 102
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 34.7 bits (76), Expect = 0.060
Identities = 15/67 (22%), Positives = 34/67 (50%)
Frame = +1
Query: 211 ITTTTTVIGVKFDKGCVIAGDTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADFQYLK 390
+ T TT++G+ F G ++ DT + G + ++C ++ + I G AD + +
Sbjct: 36 LKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVT 95
Query: 391 DIIQQKI 411
D++ ++
Sbjct: 96 DMVSSQL 102
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 34.7 bits (76), Expect = 0.060
Identities = 15/67 (22%), Positives = 34/67 (50%)
Frame = +1
Query: 211 ITTTTTVIGVKFDKGCVIAGDTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADFQYLK 390
+ T TT++G+ F G ++ DT + G + ++C ++ + I G AD + +
Sbjct: 36 LKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVT 95
Query: 391 DIIQQKI 411
D++ ++
Sbjct: 96 DMVSSQL 102
>At5g45430.1 68418.m05582 protein kinase, putative contains
similarity to male germ cell-associated kinase [Homo
sapiens] gi|23268497|gb|AAN16405
Length = 499
Score = 29.1 bits (62), Expect = 3.0
Identities = 19/55 (34%), Positives = 26/55 (47%)
Frame = +1
Query: 520 NSYVVAGIQDGEPFLGAVDKLGTAYEDAVISNGLGAYMATPLLRDAVDKGPLDQE 684
NSYV + PF V+ TA +A SNG+ Y+A P + D G Q+
Sbjct: 342 NSYVTP--KTNAPFSNGVNSYVTAKTNAPFSNGVNNYVA-PKMNAPFDNGATQQK 393
>At3g55140.2 68416.m06124 pectate lyase family protein similar to
pollen allergen Amb a 1.3 SP:P27761 from [Ambrosia
artemisiifolia]
Length = 307
Score = 28.7 bits (61), Expect = 3.9
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 433 DGLQLKPRSLHCWLTR 480
DG+Q+KP+S H W+ R
Sbjct: 97 DGIQIKPKSRHIWIDR 112
>At3g55140.1 68416.m06123 pectate lyase family protein similar to
pollen allergen Amb a 1.3 SP:P27761 from [Ambrosia
artemisiifolia]
Length = 331
Score = 28.7 bits (61), Expect = 3.9
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 433 DGLQLKPRSLHCWLTR 480
DG+Q+KP+S H W+ R
Sbjct: 121 DGIQIKPKSRHIWIDR 136
>At3g14470.1 68416.m01833 disease resistance protein (NBS-LRR class),
putative domain signature NBS-LRR exists, suggestive of a
disease resistance protein.
Length = 1054
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = -1
Query: 583 PIYQQLRERVHHPEYRQPHSCSRGDPSCSSCCRGHESTSSA 461
P+ QQ + HHP++ P S G P R ++S SS+
Sbjct: 1013 PMEQQ--PQYHHPQFHLPRSNVSGSPKSHGSHRSYDSRSSS 1051
>At1g07725.1 68414.m00833 exocyst subunit EXO70 family protein
contains Pfam domain PF03081: Exo70 exocyst complex
subunit;
Length = 566
Score = 28.3 bits (60), Expect = 5.2
Identities = 20/79 (25%), Positives = 33/79 (41%)
Frame = +1
Query: 400 QQKIIDERCVGDGLQLKPRSLHCWLTRVLYNKRSKMDPLWNSYVVAGIQDGEPFLGAVDK 579
QQ+ E +GD ++ + W+T L + S L + + +G FL + K
Sbjct: 19 QQQGFSESLIGDSVEAADAFICQWVTPHLCDSSSSSCSLSSLFSAQNRLEGRRFLEVLGK 78
Query: 580 LGTAYEDAVISNGLGAYMA 636
L A + V N A +A
Sbjct: 79 LQYAIQSTVAMNPDSAKLA 97
>At5g04140.2 68418.m00402 glutamate synthase (GLU1) /
ferredoxin-dependent glutamate synthase (Fd-GOGAT 1)
identical to ferredoxin-dependent glutamate synthase
precursor [Arabidopsis thaliana] GI:3869251
Length = 1648
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +1
Query: 520 NSYVVAGIQDGEPFLGAVDKLGTAYEDAVISNGLGAYMAT 639
N YV G+ GE + V+K+G E+A I Y AT
Sbjct: 1456 NDYVGKGMAGGEIVVTPVEKIGFVPEEATIVGNTCLYGAT 1495
>At5g04140.1 68418.m00401 glutamate synthase (GLU1) /
ferredoxin-dependent glutamate synthase (Fd-GOGAT 1)
identical to ferredoxin-dependent glutamate synthase
precursor [Arabidopsis thaliana] GI:3869251
Length = 1622
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +1
Query: 520 NSYVVAGIQDGEPFLGAVDKLGTAYEDAVISNGLGAYMAT 639
N YV G+ GE + V+K+G E+A I Y AT
Sbjct: 1430 NDYVGKGMAGGEIVVTPVEKIGFVPEEATIVGNTCLYGAT 1469
>At4g05090.1 68417.m00756 inositol monophosphatase family protein
low similarity to SP|Q42546 3'(2'),5'-bisphosphate
nucleotidase (EC 3.1.3.7) (DPNPase) {Arabidopsis
thaliana}; contains Pfam profile PF00459: Inositol
monophosphatase family
Length = 397
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 362 ETMLIFSI*RISFNKKSLMSAVLEMVSSSSPVL 460
E LIF + + SL + +LEM+SS+SP L
Sbjct: 365 ERRLIFPAGGVVVSNGSLHNQILEMISSASPTL 397
>At1g01620.1 68414.m00079 plasma membrane intrinsic protein 1C
(PIP1C) / aquaporin PIP1.3 (PIP1.3) / transmembrane
protein B (TMPB) identical to plasma membrane intrinsic
protein 1c SP:Q08733 from [Arabidopsis thaliana]
Length = 286
Score = 27.5 bits (58), Expect = 9.0
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +1
Query: 112 PSPGAFYNFPGNASTIAPSRHGVQDFTAHSASPITTTTTVIGVK 243
P P F+ PG S+ + R G+ +F A T TV+GVK
Sbjct: 34 PPPAPFFE-PGELSSWSFYRAGIAEFIATFLFLYITVLTVMGVK 76
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,752,644
Number of Sequences: 28952
Number of extensions: 338782
Number of successful extensions: 1078
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1034
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1077
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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