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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0002
         (594 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|...    28   0.89 
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica...    28   1.2  
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo...    27   2.1  
SPCC16C4.10 |||6-phosphogluconolactonase |Schizosaccharomyces po...    27   2.1  
SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz...    27   2.1  
SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces p...    26   4.8  
SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces po...    25   6.3  

>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
            subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1752

 Score = 28.3 bits (60), Expect = 0.89
 Identities = 23/73 (31%), Positives = 29/73 (39%), Gaps = 6/73 (8%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTP-----KPRPPQCSGMCPFIRPTSQAFFTTGH 367
            PTS  Y+A      P + S      S +P      P  P  S   P   PTS ++  T  
Sbjct: 1601 PTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1660

Query: 366  GYSPVL-SY*PAT 331
             YSP   SY P +
Sbjct: 1661 SYSPTSPSYSPTS 1673



 Score = 27.5 bits (58), Expect = 1.6
 Identities = 22/68 (32%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS ++  T   YSP 
Sbjct: 1594 PTSPSYSPTS-PSYSATSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT 1651

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1652 SPSYSPTS 1659



 Score = 27.5 bits (58), Expect = 1.6
 Identities = 22/68 (32%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS ++  T   YSP 
Sbjct: 1622 PTSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT 1679

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1680 SPSYSPTS 1687



 Score = 27.5 bits (58), Expect = 1.6
 Identities = 22/68 (32%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS ++  T   YSP 
Sbjct: 1636 PTSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT 1693

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1694 SPSYSPTS 1701



 Score = 27.5 bits (58), Expect = 1.6
 Identities = 22/68 (32%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS ++  T   YSP 
Sbjct: 1650 PTSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT 1707

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1708 SPSYSPTS 1715



 Score = 27.5 bits (58), Expect = 1.6
 Identities = 22/68 (32%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS ++  T   YSP 
Sbjct: 1664 PTSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT 1721

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1722 SPSYSPTS 1729



 Score = 27.5 bits (58), Expect = 1.6
 Identities = 22/68 (32%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS ++  T   YSP 
Sbjct: 1678 PTSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT 1735

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1736 SPSYSPTS 1743



 Score = 26.6 bits (56), Expect = 2.7
 Identities = 21/68 (30%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            P+S  Y+    P +   S S     S +  P  P  S   P   PTS ++  T   YSP 
Sbjct: 1573 PSSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPT 1630

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1631 SPSYSPTS 1638



 Score = 26.6 bits (56), Expect = 2.7
 Identities = 19/59 (32%), Positives = 24/59 (40%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSP 355
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS ++  T   YSP
Sbjct: 1692 PTSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSP 1748


>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
           modification enzyme|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 666

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 14/34 (41%), Positives = 19/34 (55%)
 Frame = -2

Query: 473 RSKYRPRTLLNLDLRNVLECVRSLGQLPKPSLQR 372
           RS +   +  NLDL  V+ C+  L  +PK  LQR
Sbjct: 523 RSAWDLLSFTNLDLFCVISCIPKLKDIPKRVLQR 556


>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1369

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = +2

Query: 422  EHCGGLGLGVFEACIL 469
            EHC GLG  +  AC+L
Sbjct: 1059 EHCSGLGSSITRACVL 1074


>SPCC16C4.10 |||6-phosphogluconolactonase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 257

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 3/74 (4%)
 Frame = -1

Query: 561 LFPAIMMGV*PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPP--QCSGMCPFI-RPTS 391
           +F  +++G  P    +T ++ P+H      +   A  T  P+PP  + +   P +    +
Sbjct: 140 VFDLLLLGCGPDG--HTCSLFPDHEVLQEDVAWVAPVTDSPKPPKDRITLTLPVVTHAQA 197

Query: 390 QAFFTTGHGYSPVL 349
            AF TTG G   +L
Sbjct: 198 IAFVTTGAGKKDIL 211


>SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase
           Ssp2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 576

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -3

Query: 388 SLLYNGPRVLPRLIILTRHRNDFILRE 308
           S + NG RVLP   I  RH   +I+RE
Sbjct: 12  STMRNGARVLPPEAISKRHIGPYIIRE 38


>SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1028

 Score = 25.8 bits (54), Expect = 4.8
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = -2

Query: 482 PAPRSKYRPRTLLNLDLRNVLECVRSLGQLPKPS 381
           P+P S+ RP T LN   R  +E   +  Q P P+
Sbjct: 48  PSPNSECRPLTPLNSPFRRTVEGDTANLQAPSPT 81


>SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 412

 Score = 25.4 bits (53), Expect = 6.3
 Identities = 14/57 (24%), Positives = 30/57 (52%), Gaps = 9/57 (15%)
 Frame = +2

Query: 239 IPTTGFCFELTDEQKALQELARKF---------TKDEIIPVAGQYDKTGEYPWPVVK 382
           IP   F +++ ++++A+  L+++          + D +I + GQ  +   YPW VV+
Sbjct: 226 IPVYDFPYDIEEDEEAIINLSQQLRATIPFAIVSSDRLIEMNGQTVRGRAYPWGVVE 282


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,337,750
Number of Sequences: 5004
Number of extensions: 45008
Number of successful extensions: 125
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 258201856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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