Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_P21
(927 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 194 2e-50
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 97 4e-21
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 96 7e-21
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 88 1e-18
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 58 2e-09
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 54 2e-08
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 53 7e-08
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.53
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.6
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 27 5.0
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 8.7
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 194 bits (472), Expect = 2e-50
Identities = 97/138 (70%), Positives = 103/138 (74%)
Frame = +1
Query: 292 PAIRAVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMXKIWHHTFYNELRVA 471
P +MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDM KIWHHTFYNELRVA
Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97
Query: 472 PEEHPVLLTEAPLNPKAXXEKMTXIMFETFNTPAMYVAIQSRALAXXXXXXTTGYRAWXP 651
PEEHP LLTEAPLNPK+ EKMT I+FETFN PA YVAIQ+ L+ TTG
Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQA-VLSLYASGRTTGI-VLDS 155
Query: 652 XXGVSPHRAPSTXG*ALP 705
GV+ H P G ALP
Sbjct: 156 GDGVT-HTVPIYEGYALP 172
Score = 61.7 bits (143), Expect = 1e-10
Identities = 26/27 (96%), Positives = 26/27 (96%)
Frame = +3
Query: 225 MCKAGFAGDDAPRAVFPSIVGRPRHQG 305
MCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 16 MCKAGFAGDDAPRAVFPSIVGRPRHHG 42
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 96.7 bits (230), Expect = 4e-21
Identities = 49/110 (44%), Positives = 66/110 (60%), Gaps = 1/110 (0%)
Frame = +1
Query: 307 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMXKIWHHTFYN-ELRVAPEEH 483
VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DM +IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 484 PVLLTEAPLNPKAXXEKMTXIMFETFNTPAMYVAIQSRALAXXXXXXTTG 633
P+LLTE PL EK+ +ET N PA+ ++Q LA TTG
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQP-VLALYASARTTG 155
Score = 39.9 bits (89), Expect = 5e-04
Identities = 15/24 (62%), Positives = 20/24 (83%)
Frame = +3
Query: 231 KAGFAGDDAPRAVFPSIVGRPRHQ 302
KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 22 KAGFAGDDIPKCLFPTCVGRIKHE 45
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 95.9 bits (228), Expect = 7e-21
Identities = 40/89 (44%), Positives = 63/89 (70%)
Frame = +1
Query: 328 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMXKIWHHTFYNELRVAPEEHPVLLTEAP 507
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 508 LNPKAXXEKMTXIMFETFNTPAMYVAIQS 594
+NP A EKM MFE + +YVAIQ+
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQA 138
Score = 33.1 bits (72), Expect = 0.057
Identities = 14/21 (66%), Positives = 16/21 (76%)
Frame = +3
Query: 231 KAGFAGDDAPRAVFPSIVGRP 293
K G+A D+ PR FPSIVGRP
Sbjct: 16 KVGYAKDNFPRFQFPSIVGRP 36
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 88.2 bits (209), Expect = 1e-18
Identities = 38/89 (42%), Positives = 53/89 (59%), Gaps = 1/89 (1%)
Frame = +1
Query: 331 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMXKIWHHTFYNELRVAPEEHPVLLTEAP 507
D ++G++A K +L YPI HG + NWD M + W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 508 LNPKAXXEKMTXIMFETFNTPAMYVAIQS 594
LNP E IMFE+FN +Y+A+Q+
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQA 161
Score = 29.1 bits (62), Expect = 0.93
Identities = 10/18 (55%), Positives = 15/18 (83%)
Frame = +3
Query: 231 KAGFAGDDAPRAVFPSIV 284
K G+AG+DAP VFP+++
Sbjct: 18 KLGYAGNDAPSYVFPTVI 35
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 58.0 bits (134), Expect = 2e-09
Identities = 28/78 (35%), Positives = 39/78 (50%)
Frame = +1
Query: 373 LTLKYPIEHGIVTNWDDMXKIWHHTFYNELRVAPEEHPVLLTEAPLNPKAXXEKMTXIMF 552
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP K MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 553 ETFNTPAMYVAIQSRALA 606
E+ PA Y+A Q A
Sbjct: 123 ESLRCPATYLAKQETCAA 140
Score = 26.2 bits (55), Expect = 6.6
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +3
Query: 231 KAGFAGDDAPRAVFPSIVG 287
+ GF+G+D P+ V PS G
Sbjct: 24 RIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 54.4 bits (125), Expect = 2e-08
Identities = 26/91 (28%), Positives = 50/91 (54%), Gaps = 1/91 (1%)
Frame = +1
Query: 319 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMXKIWHHTFYNELRVAPEEHPVLL 495
+ + + VG++ + G ++ + P E +++NWD M ++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 496 TEAPLNPKAXXEKMTXIMFETFNTPAMYVAI 588
TE NP MT ++FE +N P++ I
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGI 151
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 52.8 bits (121), Expect = 7e-08
Identities = 28/107 (26%), Positives = 53/107 (49%), Gaps = 2/107 (1%)
Frame = +1
Query: 292 PAIRAVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMXKIWHHTFYNELR 465
P+ V + ++ YV DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 466 VAPEEHPVLLTEAPLNPKAXXEKMTXIMFETFNTPAMYVAIQSRALA 606
V P E+ +++TE NP++ +++ FE + PA Y+ Q+ +A
Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVA 143
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.9 bits (64), Expect = 0.53
Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 5/53 (9%)
Frame = +1
Query: 448 FYNELRVAPEEHPVLLTE--APLNPKAXXEKMTXIMFETFNTPAM---YVAIQ 591
+Y L E+HP+LLT+ A L P+ ++ I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 28.3 bits (60), Expect = 1.6
Identities = 19/72 (26%), Positives = 28/72 (38%), Gaps = 4/72 (5%)
Frame = +1
Query: 388 PIEHGIVTNWDDMXKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKAXXEKMTXIMFE 555
PI+ G V +W+ + W H Y+ L P + +PV L E T FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 556 TFNTPAMYVAIQ 591
P +A +
Sbjct: 172 ECQVPGFTIAYE 183
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 26.6 bits (56), Expect = 5.0
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 304 AVMVGMGQKDSYVGDEAQSKRGILTLKYPI 393
A+M+G +Y+G AQ KR +LT + I
Sbjct: 164 AIMIGAIHGFAYIGLAAQGKRALLTARVTI 193
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 25.8 bits (54), Expect = 8.7
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +1
Query: 490 LLTEAPLNPKAXXEKMTXIMFETFN 564
+L APL+ + +KM ++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,074,667
Number of Sequences: 5004
Number of extensions: 57569
Number of successful extensions: 127
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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