BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_O06
(946 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 32 0.10
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 32 0.10
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 28 1.7
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 2.2
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.9
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.7
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 6.7
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi... 26 6.7
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 8.9
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 32.3 bits (70), Expect = 0.10
Identities = 20/72 (27%), Positives = 22/72 (30%)
Frame = +2
Query: 722 PPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXNPPXPPXX 901
PP P P P + P P P P P PP PP PP
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMG-APAAPPLPPSAPIAPPLPAGMPAAPPLPP-A 472
Query: 902 GPLPXKXPXXSP 937
P P P +P
Sbjct: 473 APAPPPAPAPAP 484
Score = 29.5 bits (63), Expect = 0.72
Identities = 17/49 (34%), Positives = 21/49 (42%)
Frame = +1
Query: 796 ALXPNPPPXXXSXXGXXXXPPXWAXXPKPPVXPXPXXLTGKKXX*XPPP 942
+L P PPP + G PP P PP P TG++ PPP
Sbjct: 335 SLPPPPPPPRSNAAGSIPLPPQGRSAPPPP-PPRSAPSTGRQ----PPP 378
Score = 26.2 bits (55), Expect = 6.7
Identities = 23/86 (26%), Positives = 24/86 (27%), Gaps = 4/86 (4%)
Frame = +2
Query: 695 PP*XPPXXX----PPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQX 862
PP PP PP S P PP P G P P P PP+
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPP---PPPRS 368
Query: 863 GXXXXNPPXPPXXGPLPXKXPXXSPP 940
P PP P PP
Sbjct: 369 APSTGRQP-PPLSSSRAVSNPPAPPP 393
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 32.3 bits (70), Expect = 0.10
Identities = 24/83 (28%), Positives = 25/83 (30%)
Frame = +2
Query: 689 GFPP*XPPXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGX 868
G PP PP PP P PS P PP P PP P P PP
Sbjct: 1187 GVPPVPPPSEAPPVPKPS-VGVPPVPPPSTAPPVPTPSAGLPP---VPVPTAKAPPVPAP 1242
Query: 869 XXXNPPXPPXXGPLPXKXPXXSP 937
P +P SP
Sbjct: 1243 SSEAPSVSTPRSSVPSPHSNASP 1265
Score = 31.9 bits (69), Expect = 0.14
Identities = 27/89 (30%), Positives = 29/89 (32%), Gaps = 5/89 (5%)
Frame = +2
Query: 689 GFPP*XPPXXXPPFPXPS-XYPXNPXP--PFXPLREXXG-PFXQTPPXXGXPXP-XXXXP 853
G PP P PP P PS P P P P+ G P P P P P
Sbjct: 1081 GAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAP 1140
Query: 854 PQXGXXXXNPPXPPXXGPLPXKXPXXSPP 940
P PP P G P P + P
Sbjct: 1141 PVPKPSVAAPPVPAPSGAPPVPKPSVAAP 1169
Score = 29.1 bits (62), Expect = 0.95
Identities = 22/82 (26%), Positives = 23/82 (28%)
Frame = +2
Query: 695 PP*XPPXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXX 874
PP P PP P PS P + P PP P PP
Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPV----PPPSEAPPVPKPSV 1205
Query: 875 XNPPXPPXXGPLPXKXPXXSPP 940
PP PP P P P
Sbjct: 1206 GVPPVPPPSTAPPVPTPSAGLP 1227
Score = 28.7 bits (61), Expect = 1.3
Identities = 22/80 (27%), Positives = 24/80 (30%), Gaps = 2/80 (2%)
Frame = +2
Query: 707 PPXXXPPFPXPSXYPXNPXPPFXP--LREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXN 880
P PP P PS P P P P + G PP P G
Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGA---- 1082
Query: 881 PPXPPXXGPLPXKXPXXSPP 940
PP P G P P + P
Sbjct: 1083 PPVPAPSGIPPVPKPSVAAP 1102
Score = 28.7 bits (61), Expect = 1.3
Identities = 25/91 (27%), Positives = 26/91 (28%), Gaps = 9/91 (9%)
Frame = +2
Query: 695 PP*XPPXXXPPFPX-----PSXYPXNPXP----PFXPLREXXGPFXQTPPXXGXPXPXXX 847
PP P PP P PS P P P P P P P P
Sbjct: 1041 PPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVA 1100
Query: 848 XPPQXGXXXXNPPXPPXXGPLPXKXPXXSPP 940
PP PP P G P P + P
Sbjct: 1101 APPVPKPSVAVPPVPAPSGAPPVPKPSVAAP 1131
Score = 27.5 bits (58), Expect = 2.9
Identities = 24/91 (26%), Positives = 26/91 (28%), Gaps = 9/91 (9%)
Frame = +2
Query: 695 PP*XPPXXXPPFPXPSXYPXNP--------XPPFXPLREXXGPFXQTPPXXGXPXPXXXX 850
PP P PP P P+ P P PP P P P
Sbjct: 1032 PPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGI 1091
Query: 851 PPQXGXXXXNPPXP-PXXGPLPXKXPXXSPP 940
PP PP P P P P +PP
Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVPAPSGAPP 1122
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 28.3 bits (60), Expect = 1.7
Identities = 18/68 (26%), Positives = 20/68 (29%)
Frame = +2
Query: 692 FPP*XPPXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXX 871
FP P P P P PP P+ G PP P PP
Sbjct: 197 FPAHHEPGEHMPPPPMHHKPGEHMPP-PPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEP 255
Query: 872 XXNPPXPP 895
+ P PP
Sbjct: 256 GEHMPPPP 263
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/62 (25%), Positives = 18/62 (29%)
Frame = +2
Query: 710 PXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXNPPX 889
P P P P PP P+ G PP P PP + P
Sbjct: 216 PGEHMPPPPMHHEPGEHMPP-PPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPP 274
Query: 890 PP 895
PP
Sbjct: 275 PP 276
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/62 (25%), Positives = 18/62 (29%)
Frame = +2
Query: 710 PXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXNPPX 889
P P P P PP P+ G PP P PP + P
Sbjct: 229 PGEHMPPPPMHHEPGEHMPP-PPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPP 287
Query: 890 PP 895
PP
Sbjct: 288 PP 289
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/62 (25%), Positives = 18/62 (29%)
Frame = +2
Query: 710 PXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXNPPX 889
P P P P PP P+ G PP P PP + P
Sbjct: 242 PGEHMPPPPMHHEPGEHMPP-PPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPP 300
Query: 890 PP 895
PP
Sbjct: 301 PP 302
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/62 (25%), Positives = 18/62 (29%)
Frame = +2
Query: 710 PXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXNPPX 889
P P P P PP P+ G PP P PP + P
Sbjct: 255 PGEHMPPPPMHHEPGEHMPP-PPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPP 313
Query: 890 PP 895
PP
Sbjct: 314 PP 315
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/62 (25%), Positives = 18/62 (29%)
Frame = +2
Query: 710 PXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXNPPX 889
P P P P PP P+ G PP P PP + P
Sbjct: 268 PGEHMPPPPMHHEPGEHMPP-PPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPP 326
Query: 890 PP 895
PP
Sbjct: 327 PP 328
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/62 (25%), Positives = 18/62 (29%)
Frame = +2
Query: 710 PXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXNPPX 889
P P P P PP P+ G PP P PP + P
Sbjct: 281 PGEHMPPPPMHHEPGEHMPP-PPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPP 339
Query: 890 PP 895
PP
Sbjct: 340 PP 341
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.9 bits (59), Expect = 2.2
Identities = 17/54 (31%), Positives = 19/54 (35%)
Frame = +2
Query: 695 PP*XPPXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPP 856
PP PP P P P+ P PP P+ P P G P PP
Sbjct: 732 PPPPPPAVIVPTPAPAPI---PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.5 bits (58), Expect = 2.9
Identities = 18/58 (31%), Positives = 20/58 (34%)
Frame = +2
Query: 722 PPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQXGXXXXNPPXPP 895
PP P P+ P P P+ P P G P P P G PP PP
Sbjct: 732 PPPPPPAVIVPTPAPA--PI-----PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.5 bits (58), Expect = 2.9
Identities = 20/70 (28%), Positives = 23/70 (32%), Gaps = 1/70 (1%)
Frame = +2
Query: 707 PPXXXPPFPXPSXYPXNPXPPFXPLREXXGPFXQTPPXXGXPXPXXXXPPQX-GXXXXNP 883
PP PP PS P +P P+ P + P P PP P
Sbjct: 139 PPTSAPP--RPSIPPPSPASA-PPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVP 195
Query: 884 PXPPXXGPLP 913
P PP P P
Sbjct: 196 PMPPKVPPPP 205
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 6.7
Identities = 12/32 (37%), Positives = 12/32 (37%)
Frame = +1
Query: 805 PNPPPXXXSXXGXXXXPPXWAXXPKPPVXPXP 900
P PPP S PP A P P P P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 6.7
Identities = 12/31 (38%), Positives = 13/31 (41%)
Frame = +2
Query: 686 QGFPP*XPPXXXPPFPXPSXYPXNPXPPFXP 778
Q PP PP P PS P + PP P
Sbjct: 1879 QAAPPPPPPMALPKAGPPSAAPTSALPPAGP 1909
>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
reductase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1053
Score = 26.2 bits (55), Expect = 6.7
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +3
Query: 483 SKAVIRLSTESGDNXGXNMLPKGQQKA 563
++ IR T +GD G NM+ KG + A
Sbjct: 794 TRLFIRFCTSTGDAMGMNMISKGVEHA 820
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.8 bits (54), Expect = 8.9
Identities = 14/37 (37%), Positives = 16/37 (43%)
Frame = +2
Query: 689 GFPP*XPPXXXPPFPXPSXYPXNPXPPFXPLREXXGP 799
G PP PP P F PS P P P + R+ P
Sbjct: 7 GNPP--PPPPPPGFEPPSQPPPPPPPGYVKKRKNKTP 41
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,203,144
Number of Sequences: 5004
Number of extensions: 31641
Number of successful extensions: 121
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 56
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 481321826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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