Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_N21
(860 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 154 1e-38
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 81 3e-16
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 30 0.49
SPCC417.15 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 28 2.0
SPAC22G7.07c |||mRNA |Schizosaccharomyces pombe|chr 1|||Manual 27 2.6
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 27 3.4
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 27 3.4
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 3.4
SPCC188.07 |ccq1||telomere maintenence protein|Schizosaccharomyc... 26 6.0
SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.0
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 26 7.9
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 154 bits (374), Expect = 1e-38
Identities = 68/120 (56%), Positives = 95/120 (79%), Gaps = 1/120 (0%)
Frame = +1
Query: 199 IKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPF 378
++FL+++KPF LPEIA PERK+ F++K+LWT +TL IFLV Q+PL+GI+SSDS+DP
Sbjct: 4 LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVMSQVPLYGIVSSDSSDPL 63
Query: 379 YWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFGM 555
W+R+ILA+NRGTLMELGISPIVTS +++QLL G+++IEV + DR ++ QK +
Sbjct: 64 LWLRMILAANRGTLMELGISPIVTSSMLVQLLVGSQLIEVNMELKSDREMYQLVQKFLAI 123
Score = 71.7 bits (168), Expect = 1e-13
Identities = 40/82 (48%), Positives = 52/82 (63%)
Frame = +3
Query: 612 PVKLVPEVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSWYPSG*LLQTSVKPLXGKAFSP 791
P L +CLL+I+QL A LIVLLLDELLQKGYGLGS S + + + + KAFSP
Sbjct: 142 PQDLGAGICLLLILQLAAASLIVLLLDELLQKGYGLGSGI-SLFIATINCENIFWKAFSP 200
Query: 792 ATVNTGRGTXFXGAVIXWFHLL 857
T + G F GAVI + +++
Sbjct: 201 TTYHIANGVQFEGAVINFVYVM 222
Score = 40.7 bits (91), Expect = 3e-04
Identities = 25/80 (31%), Positives = 38/80 (47%)
Frame = +2
Query: 539 RNYLVWVITVGXAIVYVMTGMYGEPSEIGARSVPPHYHTTVCGRSYCVVAR*IAPERIWI 718
+ +L +I G A YV+TGMYG P ++GA ++ + + +
Sbjct: 118 QKFLAIIIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLAAASLIVLLLDELLQKGYGL 177
Query: 719 GVLVSLWLIATNICETIVWK 778
G +SL+ IAT CE I WK
Sbjct: 178 GSGISLF-IATINCENIFWK 196
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 80.6 bits (190), Expect = 3e-16
Identities = 42/118 (35%), Positives = 67/118 (56%), Gaps = 1/118 (0%)
Frame = +1
Query: 196 GIKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 375
G +F+ IKP S+LPE+ P+ ++ EK+ W A + ++ + IP++G +D+ DP
Sbjct: 3 GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDP 62
Query: 376 FYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKL 546
RV+ S+ LM G++PI S ++Q+LA K I V + DR LF AQK+
Sbjct: 63 INNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKIAVNFNLIIDRVLFQNAQKV 120
Score = 36.7 bits (81), Expect = 0.004
Identities = 12/29 (41%), Positives = 25/29 (86%)
Frame = +3
Query: 639 LLIIIQLFVAGLIVLLLDELLQKGYGLGS 725
+++I+Q+F+ G++ + L E+++KG+GLGS
Sbjct: 151 IMLILQIFLPGIVCIYLCEIIEKGHGLGS 179
>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 346
Score = 29.9 bits (64), Expect = 0.49
Identities = 23/67 (34%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
Frame = -1
Query: 545 NFCAPLNKALSFGVSPTS-MILAPASNCMMSPDVTIGEIPSSINV---PLFDARITRIQ* 378
+ CA +NKAL G+ TS A C PD I + S V PL + RI ++
Sbjct: 104 SLCAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRA 160
Query: 377 KGSALSD 357
G L D
Sbjct: 161 SGRVLVD 167
>SPCC417.15 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
Length = 43
Score = 27.9 bits (59), Expect = 2.0
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +1
Query: 754 HL*NHCXERHFHQPLLTLVA 813
H +HC E HF PL+ L+A
Sbjct: 20 HFSHHCCENHFINPLVCLIA 39
>SPAC22G7.07c |||mRNA |Schizosaccharomyces pombe|chr 1|||Manual
Length = 413
Score = 27.5 bits (58), Expect = 2.6
Identities = 15/34 (44%), Positives = 17/34 (50%)
Frame = -3
Query: 828 PXIXCRDQC*QWLVKMPFXTMVSQMFVAINQRDT 727
P I DQC QWL KM S F+ I +DT
Sbjct: 121 PVIQFEDQCLQWL-KMLNTIPPSYSFITIKSKDT 153
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 27.1 bits (57), Expect = 3.4
Identities = 16/60 (26%), Positives = 30/60 (50%)
Frame = -1
Query: 560 SPIPNNFCAPLNKALSFGVSPTSMILAPASNCMMSPDVTIGEIPSSINVPLFDARITRIQ 381
+P N C +K + P ++I +P+S S ++ + PSS+NV + R+ +Q
Sbjct: 364 TPNSNGLCRNDSKCRFLMILPETIIKSPSSFIGDSYNIANIDPPSSMNVQSSEFRVGEVQ 423
>SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1588
Score = 27.1 bits (57), Expect = 3.4
Identities = 18/61 (29%), Positives = 25/61 (40%), Gaps = 2/61 (3%)
Frame = -2
Query: 721 PNPYPFWSNSSSNNTIRPATNSCMIMRRHTSG--TNFTGFAIHSSHYINNSXTNSNHPYQ 548
P P PF S SN RP IM + SG T+ G A ++ N + P+
Sbjct: 1320 PQPPPFIGESRSNRKPRPTKRQREIMEQVESGKLTSAEGAAAIAATQTRNQNNFISKPFN 1379
Query: 547 I 545
+
Sbjct: 1380 V 1380
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 3.4
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 199 IKFLEVI-KPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMS 357
I+FL+ KP+ S + R++ + K WT + I L+ PLFGI S
Sbjct: 1055 IRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFS 1108
>SPCC188.07 |ccq1||telomere maintenence protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 735
Score = 26.2 bits (55), Expect = 6.0
Identities = 13/43 (30%), Positives = 18/43 (41%)
Frame = -1
Query: 770 QWFHRCL*QLTRGIPGPQSISFLEQFIEQQHNKTGHKQLYDNE 642
Q+F C+ G + + FI+ HN H LYD E
Sbjct: 153 QYFAFCIDHFCTGPSDSRIQEKIRLFIQSAHNIAKHPSLYDTE 195
>SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 6.0
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = -1
Query: 425 SINVPLFDARITRIQ*KGSALSDDIIPNKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAI 246
S+ + LFD I+ + S +SD IP K +++ N + T+S N + +S
Sbjct: 273 SLLLGLFDKSISMLNCHSSKISDGDIPYKSHQVFLQELQNKYSEIKTYSSNKLNKSSIKS 332
Query: 245 S 243
S
Sbjct: 333 S 333
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 7.9
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -1
Query: 248 ISGNILQNGLITSKNFIPILIYC 180
+ G L +GL+ K+F+P L C
Sbjct: 83 VQGEDLDDGLLVDKHFLPTLFKC 105
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,575,024
Number of Sequences: 5004
Number of extensions: 77841
Number of successful extensions: 210
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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