Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_N03
(864 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
EF625898-1|ABR45905.1| 686|Apis mellifera hexamerin protein. 77 2e-16
EF589162-1|ABQ84439.1| 686|Apis mellifera hexamerin 70c protein. 77 3e-16
EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein. 66 3e-13
EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein. 66 4e-13
EF625896-1|ABR45903.1| 683|Apis mellifera hexamerin protein. 62 6e-12
AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein. 62 6e-12
EF625899-1|ABR45906.1| 1010|Apis mellifera high Glx storage prot... 57 2e-10
AY242387-1|AAO72539.2| 693|Apis mellifera prophenoloxidase prot... 38 2e-04
DQ325083-1|ABD14097.1| 189|Apis mellifera complementary sex det... 23 2.7
AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic ac... 22 6.3
AY350618-1|AAQ57660.1| 425|Apis mellifera complementary sex det... 22 8.4
AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 22 8.4
AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 22 8.4
>EF625898-1|ABR45905.1| 686|Apis mellifera hexamerin protein.
Length = 686
Score = 77.4 bits (182), Expect = 2e-16
Identities = 40/105 (38%), Positives = 58/105 (55%), Gaps = 2/105 (1%)
Frame = +3
Query: 552 PAPYEVYPKMFMNMEVLQKIYVTKMQHGLINPEAAAKYGIHKENDYFVYKANYSNAVL-- 725
PA YE+YP F + V+++ KM G ++ G++ Y V NYS+ +
Sbjct: 160 PAIYEIYPNYFFDSSVIEEAQNLKMSRG-----SSVVTGMNNIETYIV-NTNYSSKYMRE 213
Query: 726 YNNEEQRLTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRG 860
YN+ E +L YF ED+ +NAYYYY LP+W +S +Y KE RG
Sbjct: 214 YNDPEYKLDYFMEDVELNAYYYYMREMLPYWMSSSQYHMPKEIRG 258
Score = 59.7 bits (138), Expect = 3e-11
Identities = 27/47 (57%), Positives = 33/47 (70%)
Frame = +2
Query: 392 EAIALFHLFYYAKDFETFYKSACFARVHLNQGQFLYAFYIAVIQRPD 532
E LF L Y AKDF+TFYK+A +AR+ +N G F AF IAV+ RPD
Sbjct: 107 EVRMLFELLYNAKDFQTFYKTAAWARLRMNSGMFTTAFSIAVLYRPD 153
Score = 57.2 bits (132), Expect = 2e-10
Identities = 28/67 (41%), Positives = 42/67 (62%), Gaps = 2/67 (2%)
Frame = +1
Query: 172 FVEKQKKILSFFQDVSQLNTDD-EYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTG-FM 345
F+ KQKKI V Q + D E+Y +G++YD+E NMD Y +K V++FL Y+ G F+
Sbjct: 32 FLHKQKKIFDLLLYVRQADLSDAEWYDVGRNYDMESNMDMYKDKNVVQKFLWWYKQGMFL 91
Query: 346 PKNLEFS 366
+N F+
Sbjct: 92 SRNAIFT 98
>EF589162-1|ABQ84439.1| 686|Apis mellifera hexamerin 70c protein.
Length = 686
Score = 76.6 bits (180), Expect = 3e-16
Identities = 40/105 (38%), Positives = 58/105 (55%), Gaps = 2/105 (1%)
Frame = +3
Query: 552 PAPYEVYPKMFMNMEVLQKIYVTKMQHGLINPEAAAKYGIHKENDYFVYKANYSNAVL-- 725
PA YE+YP F + V+++ KM G ++ G++ Y V NYS+ +
Sbjct: 160 PAIYEIYPNYFFDSSVIEEAQNLKMSRG-----SSVVTGMNNIETYIV-NTNYSSKNMRE 213
Query: 726 YNNEEQRLTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRG 860
YN+ E +L YF ED+ +NAYYYY LP+W +S +Y KE RG
Sbjct: 214 YNDPEYKLDYFMEDVELNAYYYYMREMLPYWMSSSQYHMPKEIRG 258
Score = 59.7 bits (138), Expect = 3e-11
Identities = 27/47 (57%), Positives = 33/47 (70%)
Frame = +2
Query: 392 EAIALFHLFYYAKDFETFYKSACFARVHLNQGQFLYAFYIAVIQRPD 532
E LF L Y AKDF+TFYK+A +AR+ +N G F AF IAV+ RPD
Sbjct: 107 EVRMLFELLYNAKDFQTFYKTAAWARLRMNSGMFTTAFSIAVLYRPD 153
Score = 57.2 bits (132), Expect = 2e-10
Identities = 28/67 (41%), Positives = 42/67 (62%), Gaps = 2/67 (2%)
Frame = +1
Query: 172 FVEKQKKILSFFQDVSQLNTDD-EYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTG-FM 345
F+ KQKKI V Q + D E+Y +G++YD+E NMD Y +K V++FL Y+ G F+
Sbjct: 32 FLHKQKKIFDLLLYVRQADLSDAEWYDVGRNYDMESNMDMYKDKNVVQKFLWWYKQGMFL 91
Query: 346 PKNLEFS 366
+N F+
Sbjct: 92 SRNAIFT 98
>EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein.
Length = 684
Score = 66.5 bits (155), Expect = 3e-13
Identities = 41/106 (38%), Positives = 58/106 (54%), Gaps = 2/106 (1%)
Frame = +3
Query: 549 VPAPYEVYPKMFMNMEVLQKIYVTKMQHGLINPEAAAKYGIHKENDYFVYKANYSNAVLY 728
+P YE+ P F N EVLQK + G ++ + + KY KE ++ ANYS L
Sbjct: 159 LPPLYEMCPYFFFNSEVLQKANHALI-FGKLDTKTSGKY---KE---YIIPANYSGWYLN 211
Query: 729 N--NEEQRLTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRG 860
+ N E +L YF EDIG+N YY++ PFW S++Y L + RG
Sbjct: 212 HDYNLENKLNYFIEDIGLNTYYFFLRQAFPFWLPSKEYD-LPDYRG 256
Score = 64.5 bits (150), Expect = 1e-12
Identities = 26/54 (48%), Positives = 41/54 (75%)
Frame = +2
Query: 371 FYDKMRDEAIALFHLFYYAKDFETFYKSACFARVHLNQGQFLYAFYIAVIQRPD 532
+Y ++ E ALF LFY+AKDF+ F+K+A +A+ ++N+ Q++Y+ Y AVI RPD
Sbjct: 100 YYPQLLREMSALFKLFYHAKDFDIFFKTALWAKNNINEAQYIYSLYTAVITRPD 153
Score = 53.6 bits (123), Expect = 2e-09
Identities = 26/68 (38%), Positives = 43/68 (63%), Gaps = 1/68 (1%)
Frame = +1
Query: 172 FVEKQKKILSFFQDVSQ-LNTDDEYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTGFMP 348
F+ KQKK+ + V+Q + +Y G+ ++IE N+D+YTN AV+EFL +Y+ G +P
Sbjct: 33 FLLKQKKVYNLLYRVAQPALANITWYNEGQAWNIEANIDSYTNAAAVKEFLSIYKHGMLP 92
Query: 349 KNLEFSXF 372
+ FS +
Sbjct: 93 RGELFSLY 100
>EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein.
Length = 684
Score = 66.1 bits (154), Expect = 4e-13
Identities = 41/106 (38%), Positives = 58/106 (54%), Gaps = 2/106 (1%)
Frame = +3
Query: 549 VPAPYEVYPKMFMNMEVLQKIYVTKMQHGLINPEAAAKYGIHKENDYFVYKANYSNAVLY 728
+P YE+ P F N EVLQK + G ++ + + KY KE ++ ANYS L
Sbjct: 159 LPPLYEMCPYFFFNSEVLQKANHALI-FGKLDTKTSGKY---KE---YIIPANYSGWYLN 211
Query: 729 N--NEEQRLTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRG 860
+ N E +L YF EDIG+N YY++ PFW S++Y L + RG
Sbjct: 212 HDYNLENKLIYFIEDIGLNTYYFFLRQAFPFWLPSKEYD-LPDYRG 256
Score = 64.5 bits (150), Expect = 1e-12
Identities = 26/54 (48%), Positives = 41/54 (75%)
Frame = +2
Query: 371 FYDKMRDEAIALFHLFYYAKDFETFYKSACFARVHLNQGQFLYAFYIAVIQRPD 532
+Y ++ E ALF LFY+AKDF+ F+K+A +A+ ++N+ Q++Y+ Y AVI RPD
Sbjct: 100 YYPQLLREMSALFKLFYHAKDFDIFFKTALWAKNNINEAQYIYSLYTAVITRPD 153
Score = 53.6 bits (123), Expect = 2e-09
Identities = 26/68 (38%), Positives = 43/68 (63%), Gaps = 1/68 (1%)
Frame = +1
Query: 172 FVEKQKKILSFFQDVSQ-LNTDDEYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTGFMP 348
F+ KQKK+ + V+Q + +Y G+ ++IE N+D+YTN AV+EFL +Y+ G +P
Sbjct: 33 FLLKQKKVYNLLYRVAQPALANITWYNEGQAWNIEANIDSYTNAAAVKEFLSIYKHGMLP 92
Query: 349 KNLEFSXF 372
+ FS +
Sbjct: 93 RGELFSLY 100
>EF625896-1|ABR45903.1| 683|Apis mellifera hexamerin protein.
Length = 683
Score = 62.1 bits (144), Expect = 6e-12
Identities = 35/92 (38%), Positives = 51/92 (55%), Gaps = 3/92 (3%)
Frame = +3
Query: 549 VPAPYEVYPKMFMNMEVLQKIYVTKMQHGLINPEAAAKYGIHKENDYFVYKANYSNAVL- 725
+P YEV P ++ N EV+QK Y M + A + DY++ ANY+ L
Sbjct: 157 LPPMYEVMPHLYFNDEVMQKAYNIAMG------DTADMKKTYNNIDYYLLAANYTGWYLT 210
Query: 726 -YNNEEQRLTYFTEDIGMNAYYYYF-HSHLPF 815
+N EQRL YFTED+G+N +Y+ H++ PF
Sbjct: 211 KHNVPEQRLNYFTEDVGLNHFYFMLNHNYPPF 242
Score = 59.3 bits (137), Expect = 5e-11
Identities = 25/51 (49%), Positives = 36/51 (70%)
Frame = +2
Query: 380 KMRDEAIALFHLFYYAKDFETFYKSACFARVHLNQGQFLYAFYIAVIQRPD 532
+MR +A+ LF L Y AK F+ FY +A +AR ++N+ +LYA +AVI RPD
Sbjct: 101 EMRHQAVVLFRLLYSAKTFDVFYNTAVWARFNVNEQMYLYALSVAVIHRPD 151
Score = 46.8 bits (106), Expect = 3e-07
Identities = 21/66 (31%), Positives = 38/66 (57%), Gaps = 1/66 (1%)
Frame = +1
Query: 172 FVEKQKKILSFFQDVSQLNT-DDEYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTGFMP 348
+V +QK I F V Q E Y+ + +++ N+DNY +K+AV EF+++ + G +P
Sbjct: 31 YVTRQKNIYELFWHVDQPTVYHPELYQKARTFNLVENLDNYNDKEAVNEFMQLLKHGMLP 90
Query: 349 KNLEFS 366
+ F+
Sbjct: 91 RGQVFT 96
>AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein.
Length = 683
Score = 62.1 bits (144), Expect = 6e-12
Identities = 35/92 (38%), Positives = 51/92 (55%), Gaps = 3/92 (3%)
Frame = +3
Query: 549 VPAPYEVYPKMFMNMEVLQKIYVTKMQHGLINPEAAAKYGIHKENDYFVYKANYSNAVL- 725
+P YEV P ++ N EV+QK Y M + A + DY++ ANY+ L
Sbjct: 157 LPPMYEVMPHLYFNDEVMQKAYNIAMG------DTADMKKTYNNIDYYLLAANYTGWYLT 210
Query: 726 -YNNEEQRLTYFTEDIGMNAYYYYF-HSHLPF 815
+N EQRL YFTED+G+N +Y+ H++ PF
Sbjct: 211 KHNVPEQRLNYFTEDVGLNHFYFMLNHNYPPF 242
Score = 59.3 bits (137), Expect = 5e-11
Identities = 25/51 (49%), Positives = 36/51 (70%)
Frame = +2
Query: 380 KMRDEAIALFHLFYYAKDFETFYKSACFARVHLNQGQFLYAFYIAVIQRPD 532
+MR +A+ LF L Y AK F+ FY +A +AR ++N+ +LYA +AVI RPD
Sbjct: 101 EMRHQAVVLFRLLYSAKTFDVFYNTAVWARFNVNEQMYLYALSVAVIHRPD 151
Score = 46.8 bits (106), Expect = 3e-07
Identities = 21/66 (31%), Positives = 38/66 (57%), Gaps = 1/66 (1%)
Frame = +1
Query: 172 FVEKQKKILSFFQDVSQLNT-DDEYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTGFMP 348
+V +QK I F V Q E Y+ + +++ N+DNY +K+AV EF+++ + G +P
Sbjct: 31 YVTRQKNIYELFWHVDQPTVYHPELYQKARTFNLVENLDNYNDKEAVNEFMQLLKHGMLP 90
Query: 349 KNLEFS 366
+ F+
Sbjct: 91 RGQVFT 96
>EF625899-1|ABR45906.1| 1010|Apis mellifera high Glx storage protein
protein.
Length = 1010
Score = 56.8 bits (131), Expect = 2e-10
Identities = 25/51 (49%), Positives = 36/51 (70%)
Frame = +2
Query: 380 KMRDEAIALFHLFYYAKDFETFYKSACFARVHLNQGQFLYAFYIAVIQRPD 532
++R E L+ + AKD++TF K+A +ARVH+N+GQFL AF AV+ R D
Sbjct: 99 QLRKEVSLLYRILLGAKDYQTFLKTAAWARVHVNEGQFLKAFVAAVLTRQD 149
Score = 39.5 bits (88), Expect = 4e-05
Identities = 24/81 (29%), Positives = 44/81 (54%)
Frame = +3
Query: 546 VVPAPYEVYPKMFMNMEVLQKIYVTKMQHGLINPEAAAKYGIHKENDYFVYKANYSNAVL 725
+ P YE+ P+ ++ V+Q+ +Q+ G + + + + NYS L
Sbjct: 154 IFPPVYEILPQHHLDSRVIQEAQNIAIQN---------TQGKNNQQNILI-PVNYS--AL 201
Query: 726 YNNEEQRLTYFTEDIGMNAYY 788
+++EQ+L+YFT+DIG+ AYY
Sbjct: 202 LSHDEQQLSYFTQDIGLAAYY 222
Score = 36.3 bits (80), Expect = 4e-04
Identities = 19/65 (29%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = +1
Query: 175 VEKQKKILSFFQDVSQLNTDDEYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTGFM-PK 351
+ KQ+ ++ Q +SQ + E +G YDIE N Y N V + + G + P+
Sbjct: 30 LNKQQDVIQLLQKISQPIPNQELQNLGASYDIESNSHQYKNPIIVMYYAGAVKAGLVQPQ 89
Query: 352 NLEFS 366
FS
Sbjct: 90 GTTFS 94
>AY242387-1|AAO72539.2| 693|Apis mellifera prophenoloxidase
protein.
Length = 693
Score = 37.5 bits (83), Expect = 2e-04
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +2
Query: 371 FYDKMRDEAIALFHLFYYAKDFETFYKSACFARVHLNQGQFLYAFYIAVIQRPD 532
F R A L +F + +E F A + R LN F+YA +A++ RPD
Sbjct: 85 FIPAHRKIAARLIDIFMGMRTYEDFLSVAVYCRDRLNPNLFIYALSVAILHRPD 138
Score = 31.9 bits (69), Expect = 0.008
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = +3
Query: 738 EQRLTYFTEDIGMNAYYYYFHSHLPF 815
E R+ Y+ EDIG+N +++++H PF
Sbjct: 193 EHRVAYWREDIGINLHHWHWHLVYPF 218
>DQ325083-1|ABD14097.1| 189|Apis mellifera complementary sex
determiner protein.
Length = 189
Score = 23.4 bits (48), Expect = 2.7
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +3
Query: 669 IHKENDYFVYKANYSNAVLYNN 734
IH N+Y K NY+N YNN
Sbjct: 90 IHNNNNY---KYNYNNKYNYNN 108
>AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha-3 protein.
Length = 537
Score = 22.2 bits (45), Expect = 6.3
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = -3
Query: 778 FMPISSVKYVSLCSSLL 728
++P S + VSLCSS+L
Sbjct: 272 YLPSDSGEKVSLCSSIL 288
>AY350618-1|AAQ57660.1| 425|Apis mellifera complementary sex
determiner protein.
Length = 425
Score = 21.8 bits (44), Expect = 8.4
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +3
Query: 672 HKENDYFVYKANYSNAVLYNN 734
+K ++Y Y NY+N YNN
Sbjct: 322 YKYSNYNNYNNNYNNYNNYNN 342
>AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase
protein.
Length = 492
Score = 21.8 bits (44), Expect = 8.4
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +3
Query: 765 DIGMNAYYYYF 797
D GM YYY+F
Sbjct: 469 DRGMKVYYYFF 479
>AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase
protein.
Length = 628
Score = 21.8 bits (44), Expect = 8.4
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +3
Query: 765 DIGMNAYYYYF 797
D GM YYY+F
Sbjct: 469 DRGMKVYYYFF 479
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 222,150
Number of Sequences: 438
Number of extensions: 5189
Number of successful extensions: 42
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 27916710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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