Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_M22
(901 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 76 6e-15
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 67 4e-12
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 59 1e-09
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 52 1e-07
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 38 0.001
SPCC1753.05 |rsm1||RNA export factor Rsm1|Schizosaccharomyces po... 32 0.13
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 31 0.17
SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7 |Schizos... 31 0.29
SPBC18E5.01 ||SPBC29A3.19|cycloisomerase 2 family|Schizosaccharo... 28 1.6
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 28 2.1
SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Sch... 27 2.7
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 27 4.8
SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 26 8.4
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 76.2 bits (179), Expect = 6e-15
Identities = 34/96 (35%), Positives = 58/96 (60%)
Frame = +1
Query: 208 YIDGQYVDSRTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTRQ 387
++ G+++ S E+ NPAT E+IG+V + + +E A+ AA A+KT+ T + R
Sbjct: 21 FVQGKWISSPNNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAFKTYKNFTHVQRS 80
Query: 388 QLMFKFARLLRENQSKLAAKITEEQGKTIADAEGDV 495
QL+ ++A L+ EN+ L +T E GK ++ AE +V
Sbjct: 81 QLLERWAELIMENKDDLVKMLTLENGKPLSQAEMEV 116
Score = 30.3 bits (65), Expect = 0.39
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 678 ALVTGNTCIIKPSXQDPGATLMMMELLQEAGAPPG 782
AL G T I P+ + P L ++ L QEAG P G
Sbjct: 178 ALAAGCTAIFLPAFRTPYVCLGLVRLAQEAGFPDG 212
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 66.9 bits (156), Expect = 4e-12
Identities = 44/148 (29%), Positives = 74/148 (50%), Gaps = 1/148 (0%)
Frame = +1
Query: 205 LYIDGQYVDSRTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTR 384
LYI+G++ S T W E +P+ EVI +V A + E+ A+ +AK A+KTW K +
Sbjct: 23 LYINGEWHKSAET-W-ETVDPSIEEVIAKVYLAGEKEIDYAVKSAKEAFKTWKKVPGSEK 80
Query: 385 QQLMFKFARLLRENQSKLAAKITEEQGK-TIADAEGDVLRGIQSVEHCCSITSLQLGDSI 561
+L+ K A L ++ LAA + GK +++A GDV I + +C G I
Sbjct: 81 GELLMKLAELTEKHADTLAAIEAMDSGKPLVSNARGDVDGTIALLRYCAGWADKIYGQVI 140
Query: 562 QNIAKDMDTHSYKVPLGSCWRCCSLQFP 645
+ + ++ + P+G C + +P
Sbjct: 141 PTGPEKL-AYAKRTPIGVCGQIVPWNYP 167
Score = 36.3 bits (80), Expect = 0.006
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +3
Query: 663 WMFPPALVTGNTCIIKPSXQDPGATLMMMELLQEAGAPPG 782
W PAL GN IIK + P + L L++EAG P G
Sbjct: 173 WKIAPALAAGNCIIIKSAETTPLSLLYFATLVEEAGFPKG 212
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 58.8 bits (136), Expect = 1e-09
Identities = 38/150 (25%), Positives = 73/150 (48%), Gaps = 3/150 (2%)
Frame = +1
Query: 205 LYIDGQYVDSRTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKT---WSKSTV 375
L+I+ Q+VDS +++ +P+T ++I V +A ++++ A+ A+ A++T W K +
Sbjct: 25 LFINNQHVDSVHGGRVKVYSPSTEKLICEVADADEEDVDIAVKVARAAFQTDAPWRKFSS 84
Query: 376 LTRQQLMFKFARLLRENQSKLAAKITEEQGKTIADAEGDVLRGIQSVEHCCSITSLQLGD 555
R + + + A + +N LA+ T + GK+I A GDV + G
Sbjct: 85 AQRGRCLSRLADCIEQNLEYLASIETLDNGKSITLARGDVQAAADCFRYYGGWADKDYGQ 144
Query: 556 SIQNIAKDMDTHSYKVPLGSCWRCCSLQFP 645
+I+ K ++ P+G C + FP
Sbjct: 145 TIETDIKRF-AYTRHEPIGVCGQIIPWNFP 173
Score = 36.7 bits (81), Expect = 0.004
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +3
Query: 663 WMFPPALVTGNTCIIKPSXQDPGATLMMMELLQEAGAPPG 782
W PA+ GNT I+K + P + L + + + E G PPG
Sbjct: 179 WKIAPAVACGNTIILKTAELTPLSALCLTKFVPECGFPPG 218
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 52.0 bits (119), Expect = 1e-07
Identities = 34/106 (32%), Positives = 54/106 (50%), Gaps = 4/106 (3%)
Frame = +1
Query: 190 APTTKLYIDGQYVD--SRTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKT-- 357
AP L+IDG++V I L NPAT E+IG A+ ++ SA++ A +++
Sbjct: 18 APENSLFIDGKFVSPIEPAAKPIPLINPATEEIIGTCANASAKDVDSAVENAYNTFRSGI 77
Query: 358 WSKSTVLTRQQLMFKFARLLRENQSKLAAKITEEQGKTIADAEGDV 495
W+K R ++ K A+++RE + LA T GK A D+
Sbjct: 78 WAKWPGKQRGLVLRKIAKMMREKRELLAGIDTINCGKPTPYALFDI 123
Score = 35.9 bits (79), Expect = 0.008
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +3
Query: 663 WMFPPALVTGNTCIIKPSXQDPGATLMMMELLQEAGAPPG 782
W PA+ +GN ++KPS P + L + +EAG P G
Sbjct: 181 WKLVPAIASGNCVVLKPSELAPWSCLEFALICKEAGLPDG 220
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 38.3 bits (85), Expect = 0.001
Identities = 19/85 (22%), Positives = 40/85 (47%), Gaps = 1/85 (1%)
Frame = +1
Query: 265 PATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTRQQLMFKFARLLRENQSKLAA 444
P ++G V + ++ ++ A+ A K W ++ R+ + + NQ K A
Sbjct: 10 PGDGSLLGEVKLFNKSDIDQSIILAEEAQKEWKSTSFAERRNFLKALKENIIRNQDKYAE 69
Query: 445 KITEEQGKTIADAE-GDVLRGIQSV 516
++ GKT+ DA G++L ++ +
Sbjct: 70 IACKDTGKTLVDAAFGEILVTLEKI 94
>SPCC1753.05 |rsm1||RNA export factor Rsm1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 296
Score = 31.9 bits (69), Expect = 0.13
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Frame = +1
Query: 259 TNPATNEVIG---RVPEATQDELTSALDAAKRAYKTWSKSTVLTR 384
T+ TNE++ ++ E +D L +L A+K YK WS+ L R
Sbjct: 5 TDMETNEILDQLDKIDERNEDILLKSLKASKCTYKPWSREEFLRR 49
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 31.5 bits (68), Expect = 0.17
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +3
Query: 678 ALVTGNTCIIKPSXQDPGATLMMMELLQEAGAPPG 782
AL G T +I+P+ + P L + +L + AG P G
Sbjct: 183 ALAAGCTVVIRPAAETPFTALALAKLAERAGVPAG 217
>SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 721
Score = 30.7 bits (66), Expect = 0.29
Identities = 18/44 (40%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = +1
Query: 223 YVDSRTTNWIELTNPATNEVIGRV-PEATQDELTSALDAAKRAY 351
Y +RT W+EL NPA EV G + E + E T+++ A RA+
Sbjct: 498 YEPARTGEWMELLNPA--EVFGNIMVEYSYPECTTSVILALRAF 539
>SPBC18E5.01 ||SPBC29A3.19|cycloisomerase 2
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 342
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/45 (28%), Positives = 22/45 (48%)
Frame = -2
Query: 699 TCFRSPALAGTSTSGIITGKLKAATPPTTPKWHLVAMRVHVFGDV 565
TC P L + IT KL+ + PT +W + R++V ++
Sbjct: 7 TCTDGPVLVFDMETKSITEKLEGVSSPTWVEWEKKSKRLYVANEI 51
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 27.9 bits (59), Expect = 2.1
Identities = 10/30 (33%), Positives = 14/30 (46%)
Frame = +1
Query: 595 YKVPLGSCWRCCSLQFPGDDPTGGCSRQRW 684
Y S W +++P +DP RQRW
Sbjct: 249 YSYGAHSSWDARDVEYPEEDPESKADRQRW 278
>SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate
dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 27.5 bits (58), Expect = 2.7
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = +3
Query: 681 LVTGNTCIIKPSXQDPGATLMMMELLQEAGAPPG 782
L+ GN ++KPS ++ ++ ++ +EAG P G
Sbjct: 206 LLMGNVVLMKPSDHAVLSSYIVYQIFREAGLPAG 239
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/36 (33%), Positives = 22/36 (61%), Gaps = 5/36 (13%)
Frame = -2
Query: 660 SGIITGKLKAATPPTTPKW-----HLVAMRVHVFGD 568
+GI+T K + PP +PK+ HL R++++G+
Sbjct: 851 NGILTMKDSHSNPPVSPKYSSSQKHLKVERINIYGE 886
>SPCC1393.02c |||non-specific DNA binding protein Spt2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 406
Score = 25.8 bits (54), Expect = 8.4
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = -2
Query: 687 SPALAGTSTSGIITGKLKAATPPTTPK 607
+PA+ TS+SG++ K K + P +PK
Sbjct: 129 APAVNETSSSGLLQSKDKRSQSPHSPK 155
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,429,662
Number of Sequences: 5004
Number of extensions: 69849
Number of successful extensions: 178
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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