Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_M12
(877 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 313 2e-86
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 308 5e-85
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 161 1e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 133 4e-32
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 28 1.5
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 313 bits (768), Expect = 2e-86
Identities = 137/203 (67%), Positives = 166/203 (81%)
Frame = +3
Query: 147 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 326
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 327 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 506
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 507 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 686
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 687 VVEPYNSILTTHTXLEHSDCAFM 755
VVEPYNS+LTTH L+ +DC FM
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFM 203
Score = 68.5 bits (160), Expect = 1e-12
Identities = 31/39 (79%), Positives = 34/39 (87%)
Frame = +1
Query: 757 VDNEAIYDICRRNLDIERPTYTNLNRLIGXIVSSITXSL 873
VDNE+ YDICRRNLDIERP+Y NLNRLI +VSSIT SL
Sbjct: 204 VDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASL 242
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 308 bits (757), Expect = 5e-85
Identities = 139/208 (66%), Positives = 169/208 (81%), Gaps = 5/208 (2%)
Frame = +3
Query: 147 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 311
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 312 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 491
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 492 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 671
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 672 QVSTAVVEPYNSILTTHTXLEHSDCAFM 755
QVST+VVEPYNS+LTTH L++SDC FM
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFM 207
Score = 71.3 bits (167), Expect = 2e-13
Identities = 33/39 (84%), Positives = 34/39 (87%)
Frame = +1
Query: 757 VDNEAIYDICRRNLDIERPTYTNLNRLIGXIVSSITXSL 873
VDNEA YDICRRNLDIERPTY NLNRLI +VSSIT SL
Sbjct: 208 VDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASL 246
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 161 bits (391), Expect = 1e-40
Identities = 79/202 (39%), Positives = 113/202 (55%)
Frame = +3
Query: 147 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 326
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 327 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 506
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 507 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 686
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 687 VVEPYNSILTTHTXLEHSDCAF 752
VVEPYN+ L+ H +E+SD F
Sbjct: 179 VVEPYNATLSMHQLVENSDETF 200
Score = 35.9 bits (79), Expect = 0.008
Identities = 14/38 (36%), Positives = 24/38 (63%)
Frame = +1
Query: 757 VDNEAIYDICRRNLDIERPTYTNLNRLIGXIVSSITXS 870
+DNEA+ I L I+ P+Y +LN L+ +++ +T S
Sbjct: 202 IDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTS 239
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 133 bits (321), Expect = 4e-32
Identities = 72/191 (37%), Positives = 110/191 (57%), Gaps = 3/191 (1%)
Frame = +3
Query: 150 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 329
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 330 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 503
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 504 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 680
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 681 TAVVEPYNSIL 713
VV+PYNS+L
Sbjct: 180 DVVVQPYNSLL 190
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +1
Query: 757 VDNEAIYDICRRNLDIERPTYTNLNRLIGXIVSSITXSL 873
+DN A+ I L + PT+ N+L+ ++S+ T +L
Sbjct: 205 LDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTL 243
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = -2
Query: 873 QRXSDRGDNXSDETIQVGVGWALNVEITAADVIDG 769
Q+ SD ++ +D T VG+G AL I + D +DG
Sbjct: 184 QKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 226 STASSLMARCPQTRPSGVETILSTLSSARPELAST 330
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 245 WPDAHRQDHRGWRRFFQHFLQR 310
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,459,518
Number of Sequences: 5004
Number of extensions: 70362
Number of successful extensions: 219
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 202
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -