Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP27_F_L17
         (877 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosa...    29   1.1  
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    29   1.1  
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb...    28   1.5  
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S...    28   2.0  
SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ...    27   3.5  
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo...    27   3.5  
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb...    26   6.1  
SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr ...    26   8.1  

>SPAC637.08 |||iron-sulfur cluster assembly ATPase
           Nbp35|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 317

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 24/103 (23%), Positives = 41/103 (39%), Gaps = 1/103 (0%)
 Frame = +2

Query: 182 CPNFDLNCIREYFSRNSQCQLVRGPVPDPLPLSYYRVDIPNSNLTVEYHDVKTRGFDT-I 358
           CPN  +  I           + RGP  + L   + + D+   NL     D      D  +
Sbjct: 129 CPNLAVMSIGFLLPSEDSSVIWRGPKKNGLIKQFIK-DVNWENLDYLIVDTPPGTSDEHL 187

Query: 359 KIIEFYINSKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIV 487
            +++F+ NS  +  V+    Q + L   +    + RKA  PI+
Sbjct: 188 SLVQFFKNSGIDGAVVVTTPQEVALQDVRKEIDFCRKASIPIL 230


>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
 Frame = +2

Query: 170 IKXPCPN-FDLNCIREYFSRNSQCQLVRGPVPDP 268
           I+ PC + F  NCI+ +   N  C + R PV DP
Sbjct: 410 IQLPCKHYFHENCIKPWLRVNGTCAICRAPV-DP 442


>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 741

 Score = 28.3 bits (60), Expect = 1.5
 Identities = 12/37 (32%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
 Frame = +2

Query: 155 DDPKNIKXPCPN-FDLNCIREYFSRNSQCQLVRGPVP 262
           D+ +  K PC + F  NC++++   +  C L R  VP
Sbjct: 118 DEKQATKMPCGHIFGKNCLQKWLENHCTCPLCRKEVP 154


>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1052

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 16/47 (34%), Positives = 28/47 (59%)
 Frame = +2

Query: 575  EVFSYVHEINPKFILGPLLSFSLDSETQSQLGKLLNNIAVSLQEVFE 715
            + + ++H IN  F+L  LLS SL +   SQ  KLL  +++  +++ E
Sbjct: 878  KTYGHLHYIN--FVLEKLLSSSLLTYNSSQRDKLLYEVSLLFKDLQE 922


>SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase
           Cho2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 905

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -3

Query: 524 LEFHSQLPMRHFLQLALWLSYC 459
           LEF+S L  RHF+ L L   +C
Sbjct: 199 LEFNSWLVFRHFVDLILMCDFC 220


>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
           domain|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1811

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 25/90 (27%), Positives = 43/90 (47%), Gaps = 6/90 (6%)
 Frame = +2

Query: 362 IIEFYIN-----SKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLT 526
           +IE Y+N       TE +   A V   ++AS  T    +      + R D L +D     
Sbjct: 527 LIELYLNYDCISGNTENIYERAIVTLSRIASQST----SDPPPSFVFRDDQLVIDKPGF- 581

Query: 527 FTAVFPSISDL-QLSNAEVFSYVHEINPKF 613
              V+ +++D+ QL+++ + SYVH  NP +
Sbjct: 582 ---VYHTLNDIPQLNSSTIGSYVHSHNPPY 608


>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 952

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 26/114 (22%), Positives = 54/114 (47%), Gaps = 1/114 (0%)
 Frame = +2

Query: 407 AAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLTFTAVF-PSISDLQLSNAEVF 583
           A E + L+++   T F  +RK  + I   + + ++   L    +   ++ ++  +  + F
Sbjct: 76  AREEKDLQVSRRDTCFYCSRKIIKCICNEEHVGIESVQLKLILLLCQNLPNVTTNAKKYF 135

Query: 584 SYVHEINPKFILGPLLSFSLDSETQSQLGKLLNNIAVSLQEVFEAQGTILMTSY 745
           S + +    F L  L  FSLD++T SQL     + A +L E+ +    +L  ++
Sbjct: 136 SSLAD-GHNFTL-TLYKFSLDNQTFSQLLSRFKSFA-TLTELLQVHNVMLQVNF 186


>SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 678

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +2

Query: 566 SNAEVFSYVHEINPKFILGPLLSFSLDS 649
           +N  + S V  +NP  ++ PLL  SLD+
Sbjct: 44  TNRIMLSVVSSLNPDSLIAPLLCVSLDN 71


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,974,745
Number of Sequences: 5004
Number of extensions: 55851
Number of successful extensions: 151
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)