Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_L07
(838 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 3.3
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 4.4
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.4
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 4.4
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 27 4.4
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 5.8
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 5.8
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 26 7.6
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 7.6
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 7.6
>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 642
Score = 27.1 bits (57), Expect = 3.3
Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = +3
Query: 381 AKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 485
A E +++ +IER+A K A + E+ ATALRE
Sbjct: 157 ASERAREAQSSIERSASLREKQAREEAERAATALRE 192
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 26.6 bits (56), Expect = 4.4
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -3
Query: 431 LGRALDVLPRLFQSLLGLAVRVSERXPGDSW 339
+G + L RLF SL GL ++ PG+ W
Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.4
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +2
Query: 176 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 295
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 4.4
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +2
Query: 170 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 259
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 26.6 bits (56), Expect = 4.4
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +3
Query: 360 LGDANGKAKEALEQSRQNIERTAEELRKAHPD---VEKNATALREKLQA 497
+ +A KA+++LEQ+ + E L K H + E+ + +EKL A
Sbjct: 52 INEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDA 100
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 5.8
Identities = 19/64 (29%), Positives = 29/64 (45%)
Frame = +2
Query: 83 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 262
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 263 SKDA 274
SKDA
Sbjct: 230 SKDA 233
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 5.8
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = +3
Query: 393 LEQSRQNIERTAEELRKAHPDVEK 464
+EQ+R E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149
>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 25.8 bits (54), Expect = 7.6
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +3
Query: 435 LRKAHPDVEKNATALREKLQAAVXNTVQE 521
L AH D+E T++RE+L++ + +E
Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 7.6
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +2
Query: 140 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 316
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 317 LQQL 328
Q+
Sbjct: 73 NNQI 76
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 25.8 bits (54), Expect = 7.6
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -2
Query: 408 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 286
AST+ K PWP + L +P + + CS+ +E ++ +
Sbjct: 38 ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,004,860
Number of Sequences: 5004
Number of extensions: 30040
Number of successful extensions: 120
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 119
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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