Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_J18
(886 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 312 3e-86
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 175 5e-45
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 165 6e-42
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 122 5e-29
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 98 2e-21
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 92 8e-20
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 89 1e-18
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 50 3e-07
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 34 0.031
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.22
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 31 0.29
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.67
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.7
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 6.2
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 6.2
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 26 8.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 312 bits (767), Expect = 3e-86
Identities = 142/156 (91%), Positives = 149/156 (95%)
Frame = +2
Query: 143 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 322
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 323 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPXANREKMTQ 502
RGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNP +NREKMTQ
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 503 IMFETFNTPXMYVAIQAVLSLYASGRTTGIVLDSGE 610
I+FETFN P YVAIQAVLSLYASGRTTGIVLDSG+
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGD 157
Score = 84.2 bits (199), Expect = 2e-17
Identities = 39/51 (76%), Positives = 44/51 (86%)
Frame = +3
Query: 609 NGVSHTVXIYEGYALPXAILGLDLAGRDLTDYLMXILTXRGYSFTTTAXRE 761
+GV+HTV IYEGYALP AI+ LDLAGRDLTDYLM IL RGY+F+TTA RE
Sbjct: 157 DGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAERE 207
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 175 bits (427), Expect = 5e-45
Identities = 79/151 (52%), Positives = 108/151 (71%), Gaps = 1/151 (0%)
Frame = +2
Query: 161 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 340
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L +
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71
Query: 341 KYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPXANREKMTQIMFET 517
+ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ + +ET
Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131
Query: 518 FNTPXMYVAIQAVLSLYASGRTTGIVLDSGE 610
N P + ++Q VL+LYAS RTTGIVL+ G+
Sbjct: 132 LNVPALSFSLQPVLALYASARTTGIVLECGD 162
Score = 50.0 bits (114), Expect = 4e-07
Identities = 20/51 (39%), Positives = 35/51 (68%)
Frame = +3
Query: 609 NGVSHTVXIYEGYALPXAILGLDLAGRDLTDYLMXILTXRGYSFTTTAXRE 761
+G++H+V IY+G+++P AI ++ GRD+TDYL L G+ ++A +E
Sbjct: 162 DGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKE 212
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 165 bits (402), Expect = 6e-42
Identities = 77/156 (49%), Positives = 112/156 (71%), Gaps = 2/156 (1%)
Frame = +2
Query: 149 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 322
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 323 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPXANREKMTQ 502
R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM +
Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121
Query: 503 IMFETFNTPXMYVAIQAVLSLYASGRTTGIVLDSGE 610
MFE + +YVAIQAVLSLYA G ++G+V+DSG+
Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGD 157
Score = 49.6 bits (113), Expect = 6e-07
Identities = 23/51 (45%), Positives = 32/51 (62%)
Frame = +3
Query: 609 NGVSHTVXIYEGYALPXAILGLDLAGRDLTDYLMXILTXRGYSFTTTAXRE 761
+GV+H V +YE L + LD+AGRD T YL+ +L +GY+F TA E
Sbjct: 157 DGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFE 207
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 122 bits (295), Expect = 5e-29
Identities = 72/182 (39%), Positives = 100/182 (54%), Gaps = 32/182 (17%)
Frame = +2
Query: 161 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 289
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 290 ------DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPV 448
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 449 LLTEAPLNPXANREKMTQIMFETFNTPXMYVAIQAVLSLYASGRT--------TGIVLDS 604
LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186
Query: 605 GE 610
G+
Sbjct: 187 GD 188
Score = 36.7 bits (81), Expect = 0.004
Identities = 15/40 (37%), Positives = 26/40 (65%)
Frame = +3
Query: 609 NGVSHTVXIYEGYALPXAILGLDLAGRDLTDYLMXILTXR 728
+GV+H + + EGY + +I + LAGRD+T ++ +L R
Sbjct: 188 DGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDR 227
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 97.9 bits (233), Expect = 2e-21
Identities = 54/154 (35%), Positives = 83/154 (53%)
Frame = +2
Query: 146 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 325
+EV+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61
Query: 326 GILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPXANREKMTQI 505
G + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K +
Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120
Query: 506 MFETFNTPXMYVAIQAVLSLYASGRTTGIVLDSG 607
MFE+ P Y+A Q + +ASG+ T ++D G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIG 154
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 92.3 bits (219), Expect = 8e-20
Identities = 50/152 (32%), Positives = 88/152 (57%), Gaps = 3/152 (1%)
Frame = +2
Query: 161 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 340
LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++ + G ++
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGNDTLIEVGSRSI 82
Query: 341 -KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPXANREKMTQIMFET 517
+ P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE
Sbjct: 83 ARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFEL 141
Query: 518 FNTPXMYVAIQAVLSLYASGR--TTGIVLDSG 607
+N P + I + S Y + + ++GIVL+ G
Sbjct: 142 YNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLG 173
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 88.6 bits (210), Expect = 1e-18
Identities = 46/156 (29%), Positives = 85/156 (54%), Gaps = 2/156 (1%)
Frame = +2
Query: 146 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 325
EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 326 GI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPXANREKMT 499
I + +K +GI+ +W+ W +L+V P E+ +++TE NP + R+++
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121
Query: 500 QIMFETFNTPXMYVAIQAVLSLYASGRTTGIVLDSG 607
+ FE + P Y+ QAV +A+ ++T +++D G
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIG 157
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 50.4 bits (115), Expect = 3e-07
Identities = 29/91 (31%), Positives = 44/91 (48%), Gaps = 4/91 (4%)
Frame = +2
Query: 347 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPXANREKMTQIMFE 514
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 515 TFNTPXMYVAIQAVLSLYASGRTTGIVLDSG 607
P +A + ++ LYA G G+V+D G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIG 202
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 33.9 bits (74), Expect = 0.031
Identities = 30/124 (24%), Positives = 54/124 (43%), Gaps = 4/124 (3%)
Frame = +2
Query: 161 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 340
+V+DNG+ KAGFAG P+ + R + G + ++G+E + TL
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57
Query: 341 KYPIEH--GIVTNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPXANREKMTQIM 508
++ H G + +W +W N + P ++ +LLT+ + Q++
Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117
Query: 509 FETF 520
FE F
Sbjct: 118 FEEF 121
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.22
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +2
Query: 206 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 349
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.7 bits (66), Expect = 0.29
Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 5/53 (9%)
Frame = +2
Query: 407 FYNELRVAPEEHPVLLTE--APLNPXANREKMTQIMFETFNTPXM---YVAIQ 550
+Y L E+HP+LLT+ A L P + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.5 bits (63), Expect = 0.67
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%)
Frame = -2
Query: 303 PTYESFCPM-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 142
PT S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 27.5 bits (58), Expect = 2.7
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +1
Query: 634 STKDTHSPXPSWVWTW 681
STK P PSW W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
Tor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2337
Score = 26.2 bits (55), Expect = 6.2
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -1
Query: 343 FEGQDTSFALCFVSYVRVLLSHADHHA 263
+EG+DT+ F +Y+R++L D A
Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 6.2
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -2
Query: 306 SPTYESFC--PMPTITP*WRGLPTIEGNTARGASSPAKPAL 190
S T +SF P+PT P LPT NT + P+ PAL
Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.8 bits (54), Expect = 8.2
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 200 NRPCTYRSHCLLPTRQLP 147
NRP YRSH + TR+ P
Sbjct: 323 NRPSNYRSHSSMGTRRSP 340
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,333,142
Number of Sequences: 5004
Number of extensions: 70045
Number of successful extensions: 211
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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