BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_J05
(916 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 33 0.057
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 32 0.13
SPAC4F8.11 |||WD repeat protein, human WDR24 family|Schizosaccha... 30 0.53
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 27 3.7
SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces pomb... 27 4.9
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 6.5
SPBC4F6.16c |ero11||ER oxidoreductin Ero1a|Schizosaccharomyces p... 26 6.5
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 33.1 bits (72), Expect = 0.057
Identities = 13/42 (30%), Positives = 25/42 (59%)
Frame = +2
Query: 224 DYDSAAHNTKYNQYLKMSTSTCNCNARDRVVYGGNSADSTRE 349
++ + +++ YN+ M TS+C+C++ + YGGN A E
Sbjct: 41 EFTNVGYSSTYNEITNMDTSSCSCSSTPK-SYGGNLAPFDEE 81
>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
Mug36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1646
Score = 31.9 bits (69), Expect = 0.13
Identities = 17/57 (29%), Positives = 32/57 (56%)
Frame = +2
Query: 176 SNQDLEDKLYNSILTGDYDSAAHNTKYNQYLKMSTSTCNCNARDRVVYGGNSADSTR 346
++QD ED++ N L + D ++HN + L+ ++ + +D +VY G + STR
Sbjct: 149 ASQDFEDEVINEGLESEIDGSSHNL-LDSILQKCLASTSVLMQDALVYIGTANMSTR 204
>SPAC4F8.11 |||WD repeat protein, human WDR24
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 846
Score = 29.9 bits (64), Expect = 0.53
Identities = 10/37 (27%), Positives = 22/37 (59%)
Frame = +2
Query: 224 DYDSAAHNTKYNQYLKMSTSTCNCNARDRVVYGGNSA 334
+Y +HN +N + + S ++ + +R+ V+ GNS+
Sbjct: 611 NYSGTSHNEMFNSFHRSSVTSASIKSREAVLSAGNSS 647
>SPBC12C2.02c |ste20|ste16|sterility protein
Ste20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1309
Score = 27.1 bits (57), Expect = 3.7
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -2
Query: 450 AFTIVSSSKASLNSRL*MKNRTSFSYLAGWKNHCSLVLS 334
+ T +S+ S S L K T FSY AG + CS+ +S
Sbjct: 117 SLTRSASATVSRKSSLQEKYSTRFSYKAGCSDSCSVTVS 155
>SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +2
Query: 302 RDRVVYGGNSADSTREQWFFQPAKYENDVLFFIYN 406
R R+V G ++A + + W F +Y ++F+++N
Sbjct: 162 RQRIVVGKHAAHFSLDHWIF-VVEYYAPIVFYVFN 195
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.2 bits (55), Expect = 6.5
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +2
Query: 137 AGVVELSADSMSPSNQDLEDKLYNSILTGDYDSA 238
A +V+ S +P + DLE + ++LTGD SA
Sbjct: 537 AEIVKDSFKIFNPEDSDLEKNITEALLTGDVLSA 570
>SPBC4F6.16c |ero11||ER oxidoreductin Ero1a|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 467
Score = 26.2 bits (55), Expect = 6.5
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +2
Query: 164 SMSPSNQDLEDKLYNSILTGDYDSAAHNTKYNQY 265
S+SPS LE +++N I++G + S + + N Y
Sbjct: 205 SLSPSVACLEKRMFNRIISGFHASISTHVCQNYY 238
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,895,701
Number of Sequences: 5004
Number of extensions: 50283
Number of successful extensions: 148
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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