BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_I24
(920 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.40
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 0.70
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.93
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.6
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 23 5.7
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 6.5
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 8.6
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 30.3 bits (65), Expect = 0.40
Identities = 22/69 (31%), Positives = 22/69 (31%), Gaps = 2/69 (2%)
Frame = +1
Query: 616 SXGXPXGPAPXGA-XFXVXXXXGPPPPXRSXXXPP-PXXGGAXPXGPXXXXGRXPPXXXP 789
S P PAP GA PP P S PP P P P PP P
Sbjct: 1146 SVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVP--PVPPPSEAPPVPKP 1203
Query: 790 PXXXPXXPP 816
P PP
Sbjct: 1204 SVGVPPVPP 1212
Score = 28.3 bits (60), Expect = 1.6
Identities = 22/67 (32%), Positives = 22/67 (32%), Gaps = 2/67 (2%)
Frame = +1
Query: 622 GXPXGPAPXGAXFXVXXXXGPPP-PXRSXXXPP-PXXGGAXPXGPXXXXGRXPPXXXPPX 795
G P P P A V G PP P S PP P P P G PP P
Sbjct: 1138 GAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAG-VPPVPPPSE 1196
Query: 796 XXPXXPP 816
P P
Sbjct: 1197 APPVPKP 1203
Score = 27.5 bits (58), Expect = 2.8
Identities = 16/62 (25%), Positives = 17/62 (27%)
Frame = +3
Query: 627 PXXPRPXRGAVXGXXPPGPPPPXPXXXXXPPXXGXGXPXGAPXXXRXXPPXXXPPXXXPL 806
P P+P A P G PP PP P PP P P
Sbjct: 1121 PPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPP 1180
Query: 807 XP 812
P
Sbjct: 1181 VP 1182
Score = 27.5 bits (58), Expect = 2.8
Identities = 18/63 (28%), Positives = 19/63 (30%), Gaps = 1/63 (1%)
Frame = +3
Query: 627 PXXPRPXRGAVXGXXPPGPPPPXP-XXXXXPPXXGXGXPXGAPXXXRXXPPXXXPPXXXP 803
P P+P G V PP PP P PP P PP P P
Sbjct: 1179 PPVPKPAAG-VPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAP 1237
Query: 804 LXP 812
P
Sbjct: 1238 PVP 1240
Score = 26.6 bits (56), Expect = 4.9
Identities = 20/67 (29%), Positives = 20/67 (29%), Gaps = 1/67 (1%)
Frame = +1
Query: 616 SXGXPXGPAPXGAXFXVXXXX-GPPPPXRSXXXPPPXXGGAXPXGPXXXXGRXPPXXXPP 792
S G P P P V PP P S PP P P G PP P
Sbjct: 1175 SSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAG-LPPVPVPT 1233
Query: 793 XXXPXXP 813
P P
Sbjct: 1234 AKAPPVP 1240
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.5 bits (63), Expect = 0.70
Identities = 14/45 (31%), Positives = 15/45 (33%)
Frame = +1
Query: 682 PPPPXRSXXXPPPXXGGAXPXGPXXXXGRXPPXXXPPXXXPXXPP 816
PPPP + P P P G PP PP PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPP 776
Score = 29.1 bits (62), Expect = 0.93
Identities = 13/24 (54%), Positives = 13/24 (54%)
Frame = +3
Query: 627 PXXPRPXRGAVXGXXPPGPPPPXP 698
P P P G V G PP PPPP P
Sbjct: 761 PPPPPPPPG-VAGAGPPPPPPPPP 783
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/28 (39%), Positives = 12/28 (42%)
Frame = +3
Query: 636 PRPXRGAVXGXXPPGPPPPXPXXXXXPP 719
P P + G PP PPPP PP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/31 (38%), Positives = 14/31 (45%)
Frame = +3
Query: 600 PIKXLVXRXPXXPRPXRGAVXGXXPPGPPPP 692
P ++ P P P A G PP PPPP
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 25.8 bits (54), Expect = 8.6
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +2
Query: 446 PPPPGGGGFXXXAPFPXPP 502
PPPPG G P P PP
Sbjct: 765 PPPPGVAGAGPPPPPPPPP 783
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.1 bits (62), Expect = 0.93
Identities = 18/62 (29%), Positives = 20/62 (32%), Gaps = 2/62 (3%)
Frame = +1
Query: 637 PAPXGAXFXVXXXXGPPP--PXRSXXXPPPXXGGAXPXGPXXXXGRXPPXXXPPXXXPXX 810
P P + V PPP P RS PP + P P PP P
Sbjct: 376 PPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSA 435
Query: 811 PP 816
PP
Sbjct: 436 PP 437
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/38 (34%), Positives = 13/38 (34%)
Frame = +2
Query: 680 APPPRXXPXXXPPXXXXGPPXXGPXXXXXXPPXXXPPL 793
APP P PP PP P P PPL
Sbjct: 1698 APPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPL 1735
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 22.6 bits (46), Expect(2) = 5.7
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +3
Query: 672 PPGPPPPXP 698
PP PPPP P
Sbjct: 945 PPPPPPPPP 953
Score = 21.8 bits (44), Expect(2) = 5.7
Identities = 7/14 (50%), Positives = 8/14 (57%)
Frame = +3
Query: 651 GAVXGXXPPGPPPP 692
G + PP PPPP
Sbjct: 939 GVMPAFPPPPPPPP 952
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 6.5
Identities = 15/49 (30%), Positives = 15/49 (30%), Gaps = 2/49 (4%)
Frame = +3
Query: 672 PPGPPPPXPXXXXXPPXXGXGXPXGAPXXXRXXP--PXXXPPXXXPLXP 812
P P PP P PP P P P P PP L P
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPP 173
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 25.8 bits (54), Expect = 8.6
Identities = 23/81 (28%), Positives = 24/81 (29%), Gaps = 3/81 (3%)
Frame = -1
Query: 911 GGAXRXRKXPGGXXXXDXXPGXXXXEXXXXXXGGXXGXXXGGXXXGGXRP---XXXXGPX 741
GG+ PGG G GG G GG G P G
Sbjct: 195 GGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGF 254
Query: 740 GXAPPXXGGGXXXXRXGGGGP 678
G P GGG G GGP
Sbjct: 255 GGGPGGFGGGP----GGHGGP 271
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,774,026
Number of Sequences: 5004
Number of extensions: 24386
Number of successful extensions: 133
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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