Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_I22
(903 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 457 e-131
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 446 e-127
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 339 2e-95
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 306 2e-85
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 2e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.6
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.8
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 5.0
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 457 bits (1127), Expect = e-131
Identities = 215/221 (97%), Positives = 216/221 (97%)
Frame = +1
Query: 121 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 300
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 301 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 480
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 481 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 660
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 661 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKXG 783
IGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERK G
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEG 221
Score = 29.5 bits (63), Expect = 0.044
Identities = 15/26 (57%), Positives = 15/26 (57%)
Frame = +2
Query: 806 EXLDAILAXGRPXXXPLXXPLQXVYK 883
E LDAIL RP L PLQ VYK
Sbjct: 230 EALDAILPPTRPTDKALRLPLQDVYK 255
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 446 bits (1099), Expect = e-127
Identities = 208/221 (94%), Positives = 213/221 (96%)
Frame = +1
Query: 121 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 300
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 301 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 480
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 481 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 660
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 661 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKXG 783
IGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+VERK G
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDG 221
Score = 30.3 bits (65), Expect = 0.025
Identities = 15/26 (57%), Positives = 15/26 (57%)
Frame = +2
Query: 806 EXLDAILAXGRPXXXPLXXPLQXVYK 883
E LDAIL RP L PLQ VYK
Sbjct: 230 EALDAILPPSRPTDKALRLPLQDVYK 255
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 339 bits (833), Expect = 2e-95
Identities = 159/164 (96%), Positives = 160/164 (97%)
Frame = +1
Query: 292 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 471
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 472 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 651
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 652 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKXG 783
IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERK G
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEG 164
Score = 29.5 bits (63), Expect = 0.044
Identities = 15/26 (57%), Positives = 15/26 (57%)
Frame = +2
Query: 806 EXLDAILAXGRPXXXPLXXPLQXVYK 883
E LDAIL RP L PLQ VYK
Sbjct: 173 EALDAILPPTRPTDKALRLPLQDVYK 198
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 306 bits (751), Expect = 2e-85
Identities = 143/148 (96%), Positives = 144/148 (97%)
Frame = +1
Query: 340 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 519
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 520 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 699
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 700 SGWHGDNMLEPSTKMPWFKGWQVERKXG 783
SGWHGDNMLE S+KMPWFKGW VERK G
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEG 148
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 2e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +1
Query: 379 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 558
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 559 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 729
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.71
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 133 KTHINIVVIGHVDSGKST 186
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.6
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 142 INIVVIGHVDSGKST 186
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 364 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 465
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 5.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 364 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 465
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 240,840
Number of Sequences: 438
Number of extensions: 4879
Number of successful extensions: 20
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20
length of database: 146,343
effective HSP length: 58
effective length of database: 120,939
effective search space used: 29267238
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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