Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_H11
(884 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 219 6e-58
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 137 2e-33
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 132 5e-32
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 87 2e-18
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 59 7e-10
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 58 1e-09
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 51 2e-07
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 48 2e-06
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 39 8e-04
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 36 0.008
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 4.7
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 219 bits (534), Expect = 6e-58
Identities = 105/122 (86%), Positives = 112/122 (91%)
Frame = +3
Query: 465 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 644
EKMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+
Sbjct: 117 EKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIM 176
Query: 645 RLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXEMATXXXSXXL 824
RLDLAGRDLTDYLMKIL ERGY+F+TTAE EIVRDIKEKLCYVALDFE E+ T S L
Sbjct: 177 RLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSL 236
Query: 825 EK 830
EK
Sbjct: 237 EK 238
Score = 159 bits (387), Expect = 4e-40
Identities = 69/74 (93%), Positives = 72/74 (97%)
Frame = +2
Query: 242 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 421
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 422 VLLTEAPLNPKANR 463
LLTEAPLNPK+NR
Sbjct: 103 CLLTEAPLNPKSNR 116
Score = 84.2 bits (199), Expect = 2e-17
Identities = 35/41 (85%), Positives = 40/41 (97%)
Frame = +1
Query: 118 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 240
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
Score = 38.7 bits (86), Expect = 0.001
Identities = 16/18 (88%), Positives = 18/18 (100%)
Frame = +1
Query: 826 KKSYELPDGQVITIGNEK 879
+KSYELPDGQVITIGNE+
Sbjct: 237 EKSYELPDGQVITIGNER 254
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 137 bits (332), Expect = 2e-33
Identities = 62/110 (56%), Positives = 82/110 (74%)
Frame = +3
Query: 465 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 644
EKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H +
Sbjct: 117 EKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVG 176
Query: 645 RLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXE 794
RLD+AGRD T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E +
Sbjct: 177 RLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELD 226
Score = 81.0 bits (191), Expect = 2e-16
Identities = 32/67 (47%), Positives = 51/67 (76%)
Frame = +2
Query: 263 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 442
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 443 LNPKANR 463
+NP ANR
Sbjct: 110 MNPVANR 116
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +1
Query: 130 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 228
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
Score = 27.1 bits (57), Expect = 3.6
Identities = 9/17 (52%), Positives = 15/17 (88%)
Frame = +1
Query: 829 KSYELPDGQVITIGNEK 879
++Y LPDG+VI +G+E+
Sbjct: 238 RNYTLPDGRVIKVGSER 254
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 132 bits (320), Expect = 5e-32
Identities = 59/111 (53%), Positives = 84/111 (75%)
Frame = +3
Query: 465 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 644
EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI
Sbjct: 122 EKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQ 181
Query: 645 RLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXEM 797
+ ++ GRD+TDYL L + G+ ++AE EIVR+IKEK CYVA DF E+
Sbjct: 182 QEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEI 232
Score = 82.6 bits (195), Expect = 7e-17
Identities = 38/69 (55%), Positives = 49/69 (71%), Gaps = 1/69 (1%)
Frame = +2
Query: 242 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEH 418
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 419 PVLLTEAPL 445
P+LLTE PL
Sbjct: 107 PLLLTEPPL 115
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +1
Query: 124 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 237
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
Score = 25.8 bits (54), Expect = 8.2
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +1
Query: 832 SYELPDGQVITIGNE 876
+Y+LPD Q IT+G E
Sbjct: 244 TYQLPDNQTITLGTE 258
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 87.4 bits (207), Expect = 2e-18
Identities = 47/120 (39%), Positives = 70/120 (58%), Gaps = 8/120 (6%)
Frame = +3
Query: 465 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEG 620
E +IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ EG
Sbjct: 140 ENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEG 199
Query: 621 YALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXEMA 800
Y + +I + LAGRD+T ++ +L +R + + IKE+ CYV D E +
Sbjct: 200 YVIGSSIKTMPLAGRDVTYFVQSLLRDRN---EPDSSLKTAERIKEECCYVCPDIVKEFS 256
Score = 77.0 bits (181), Expect = 3e-15
Identities = 40/98 (40%), Positives = 55/98 (56%), Gaps = 4/98 (4%)
Frame = +2
Query: 182 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 349
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 350 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 463
WD ME+ W + + LR PE+H LLTE PLNP NR
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENR 139
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 219
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 59.3 bits (137), Expect = 7e-10
Identities = 27/77 (35%), Positives = 49/77 (63%)
Frame = +3
Query: 567 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVR 746
+V+DSG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVN 216
Query: 747 DIKEKLCYVALDFEXEM 797
+IKE +CYV+ +F+ +M
Sbjct: 217 EIKESVCYVSQNFKEDM 233
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 58.4 bits (135), Expect = 1e-09
Identities = 31/122 (25%), Positives = 60/122 (49%), Gaps = 2/122 (1%)
Frame = +3
Query: 471 MTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAIL 644
MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH +P+ G +
Sbjct: 134 MTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAK 193
Query: 645 RLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXEMATXXXSXXL 824
R+ G + YL+K+ + SF + C+V+ D+ ++A L
Sbjct: 194 RISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDYTHDIAHALDRDIL 253
Query: 825 EK 830
E+
Sbjct: 254 ER 255
Score = 42.7 bits (96), Expect = 7e-05
Identities = 21/67 (31%), Positives = 39/67 (58%), Gaps = 1/67 (1%)
Frame = +2
Query: 254 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 430
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 431 TEAPLNP 451
TE NP
Sbjct: 121 TEPLANP 127
Score = 37.5 bits (83), Expect = 0.003
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +1
Query: 58 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 231
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 51.2 bits (117), Expect = 2e-07
Identities = 30/98 (30%), Positives = 47/98 (47%)
Frame = +3
Query: 465 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 644
E TQ FE P +A + ++ LYA G G+V+D G + PI +G + A
Sbjct: 163 ELATQFFFEECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQ 222
Query: 645 RLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKE 758
+L L GR +T +L +L E + ++ + DI E
Sbjct: 223 QLPLGGRYMTQHLQNLLRESLPTLKSSGQYVSKEDITE 260
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 47.6 bits (108), Expect = 2e-06
Identities = 24/81 (29%), Positives = 40/81 (49%)
Frame = +3
Query: 468 KMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 647
K + MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L
Sbjct: 116 KTLETMFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQV 175
Query: 648 LDLAGRDLTDYLMKILTERGY 710
+G + D L + L ++ +
Sbjct: 176 QHFSGNAINDILAQTLRDKNF 196
Score = 46.0 bits (104), Expect = 7e-06
Identities = 19/52 (36%), Positives = 29/52 (55%)
Frame = +2
Query: 308 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 463
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENR 114
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +1
Query: 121 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 294
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 39.1 bits (87), Expect = 8e-04
Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 2/69 (2%)
Frame = +2
Query: 263 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 436
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 437 APLNPKANR 463
NP++ R
Sbjct: 109 PSWNPQSVR 117
Score = 34.3 bits (75), Expect = 0.023
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +1
Query: 121 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 222
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 35.9 bits (79), Expect = 0.008
Identities = 17/84 (20%), Positives = 42/84 (50%)
Frame = +3
Query: 465 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 644
EK+ I+F + + +++ + + +G + V+D G + + EG +P++ +
Sbjct: 260 EKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNSRI 319
Query: 645 RLDLAGRDLTDYLMKILTERGYSF 716
+++ G D+T MK+L + +
Sbjct: 320 KINYGGDDITLLFMKLLMRSHFPY 343
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 4.7
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +1
Query: 610 STKVTLCPTPSSVWTW 657
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -2
Query: 346 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 233
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,183,910
Number of Sequences: 5004
Number of extensions: 63054
Number of successful extensions: 235
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -