Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_F02
(923 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.12c |||triglyceride lipase-cholesterol esterase |Schizo... 71 3e-13
SPBC14C8.15 |||triglyceride lipase-cholesterol esterase |Schizos... 66 9e-12
SPCC1672.09 |||triglyceride lipase-cholesterol esterase |Schizos... 64 3e-11
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 27 2.8
SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces po... 27 3.7
SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 27 4.9
SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificit... 26 6.5
SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosacc... 26 6.5
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 26 8.6
SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 26 8.6
SPAC5D6.13 ||SPAPJ735.02c|Golgi phosphoprotein 3 family|Schizosa... 26 8.6
>SPBC16A3.12c |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 443
Score = 70.5 bits (165), Expect = 3e-13
Identities = 37/97 (38%), Positives = 54/97 (55%), Gaps = 2/97 (2%)
Frame = +2
Query: 359 GYRCETHSLISQ-GYVLNIHRIPQARSGGDTPSNTVILQHGLFASSADWV-LNGPGKSLA 532
GYR E H + +Q ++L +HRI + V HGL +S WV +N +SL
Sbjct: 83 GYRVEEHLVRTQDNFILCLHRITHPKQS-QHKREVVYCHHGLMTNSELWVAVNESERSLP 141
Query: 533 FVLADAGYDVWMPNIRGNRYSXEHTTFXEQFDSILXF 643
FVL ++GYDVW+ N RGN+YS +H T+ + + F
Sbjct: 142 FVLIESGYDVWLGNNRGNKYSRKHITYKPKDEEFWNF 178
>SPBC14C8.15 |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 65.7 bits (153), Expect = 9e-12
Identities = 34/93 (36%), Positives = 52/93 (55%), Gaps = 5/93 (5%)
Frame = +2
Query: 359 GYRCETHSL-ISQGYVLNIHRIPQARSGGDTPSN---TVILQHGLFASSADWVLNGPGK- 523
GY E H + + GY+L +HR+ + + G N V+ HGL +S WV N +
Sbjct: 83 GYDLEEHFVRTTDGYLLGLHRVYKKKKGKIEELNYLPPVLFIHGLMMNSESWVCNLKKED 142
Query: 524 SLAFVLADAGYDVWMPNIRGNRYSXEHTTFXEQ 622
++ F L + GYDVW+ N+RGN+YS ++ F Q
Sbjct: 143 AIPFALVEQGYDVWLGNLRGNKYSIKNIKFSSQ 175
>SPCC1672.09 |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 467
Score = 64.1 bits (149), Expect = 3e-11
Identities = 38/92 (41%), Positives = 48/92 (52%), Gaps = 7/92 (7%)
Frame = +2
Query: 359 GYRCETHSLISQG-YVLNIHRIPQ---ARSGGDTPSN--TVILQHGLFASSADWVLN-GP 517
GY E H + ++ Y+L IHRI + R G P V HGL +S WV N P
Sbjct: 89 GYYVEDHLVRTEDDYILCIHRISKDSPGRIGSPHPKKLPVVYCHHGLLMNSEVWVCNVDP 148
Query: 518 GKSLAFVLADAGYDVWMPNIRGNRYSXEHTTF 613
L F L + GYDVW+ N RGN+YS +H F
Sbjct: 149 RNCLVFDLVNKGYDVWLGNNRGNKYSRQHLRF 180
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/44 (25%), Positives = 26/44 (59%)
Frame = +2
Query: 179 FEDARNYIETQKDKVIEEWSSYMEDIKLSSSWTNFLEDKENPSL 310
F + R ++ ++ + +I + +Y+E ++ +S+ LE +EN L
Sbjct: 524 FAEDRKFLNSRGNLIIRQLCNYIEGERVYTSFAGILETEENLEL 567
>SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 412
Score = 27.1 bits (57), Expect = 3.7
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2
Query: 173 NNFEDARNYIETQKDKVIEEWS 238
NNF+ YIE+Q D V+EE S
Sbjct: 130 NNFDIILQYIESQYDNVLEEES 151
>SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 26.6 bits (56), Expect = 4.9
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +3
Query: 492 LPTGS*TGLENP*PSCWRTPATTSGCQT*EVTDIRGSTPLXXSSSTQY 635
LP S + + P + +R+ +TTS +T TP+ SSS Y
Sbjct: 156 LPNASGSSVYQPPYTPFRSVSTTSSSNDTSITPFGDQTPILSSSSDPY 203
>SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ysh1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 6.5
Identities = 16/56 (28%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
Frame = +2
Query: 161 EASXNNF-EDARNYIETQKDKVIEEWSSYMEDIKLSSSWTNFLEDKENPSLEDPDV 325
EAS N+ ED E+ DK+ E+ + +D+K + E K E P++
Sbjct: 714 EASQNDVSEDDFENEESDDDKIFEQQTKIEDDVKNENKTEPVEEQKSEEKNEQPNL 769
>SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein
4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 636
Score = 26.2 bits (55), Expect = 6.5
Identities = 20/88 (22%), Positives = 37/88 (42%), Gaps = 1/88 (1%)
Frame = +2
Query: 188 ARNYIETQKDKVIEEWSSYMEDIKLSSSWTNFLEDKENPSLEDPDVVLSVPAMITRRGYR 367
A N + +++++ +E + + L SW N ++ +DP V A + R
Sbjct: 296 AGNSKKREEERIAKEAADAQHQLSLVQSWINGYGQEKLADKKDP---AKVSASFVEKYGR 352
Query: 368 C-ETHSLISQGYVLNIHRIPQARSGGDT 448
C E + G V H++ SG +T
Sbjct: 353 CQEVIGRGAFGVVRIAHKVDPQNSGSET 380
>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1405
Score = 25.8 bits (54), Expect = 8.6
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = +2
Query: 191 RNYIETQKDKVIEEWSSYMEDIKLSSSWTNFLEDKENPSLED 316
R++IET + VIE + Y+ ++ LE+ + L+D
Sbjct: 958 RDFIETIRTFVIENIAKYLASVRERRDLAPMLEEPDMDDLDD 999
>SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual
Length = 510
Score = 25.8 bits (54), Expect = 8.6
Identities = 27/113 (23%), Positives = 45/113 (39%), Gaps = 2/113 (1%)
Frame = +2
Query: 140 LICSEAREASXNNF-EDARNYIETQKDKVIEEWSSYMEDIKLSSSWTNFLEDKENPSLED 316
L+ + E S ++ N + +Q + V D K SS + ++ E P++
Sbjct: 288 LLINVIEECSNSDLIHSGTNVVSSQPNVVESHVPPSSNDTKQESSLIDLMKLTEEPAVPS 347
Query: 317 PDVVLSVPAMITRRGYRCETHSLIS-QGYVLNIHRIPQARSGGDTPSNTVILQ 472
P + +VPA + ++S+ S LN QA S T ILQ
Sbjct: 348 PSLPTNVPANQSLSMLSSLSNSMSSTSNGALNSPSYSQAAIPNTNSSLTSILQ 400
>SPAC5D6.13 ||SPAPJ735.02c|Golgi phosphoprotein 3
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 337
Score = 25.8 bits (54), Expect = 8.6
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 5/55 (9%)
Frame = +2
Query: 122 CLRSKGLICSEAREASXNNFEDARNYIETQK-----DKVIEEWSSYMEDIKLSSS 271
CLR+ L CS N D NY + +K D+++ ++S + D+ SSS
Sbjct: 242 CLRTVALACSSYAANVLENALDNLNYEDREKAFSRVDELLCDFSQWPFDLHASSS 296
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,869,313
Number of Sequences: 5004
Number of extensions: 53201
Number of successful extensions: 136
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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