Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_D20
(903 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 168 1e-42
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 168 1e-42
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 108 1e-24
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 108 1e-24
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 108 1e-24
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 68 2e-12
SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 39 8e-04
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 38 0.001
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 38 0.002
SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 31 0.30
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 4.8
SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 27 4.8
>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 168 bits (408), Expect = 1e-42
Identities = 83/136 (61%), Positives = 96/136 (70%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXX 327
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 13 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 72
Query: 328 XXXXXXXXXXXNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFM 507
Y+TP LAVL++YKV+++G + RLRREC CGA FM
Sbjct: 73 LRGGGKKRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFM 130
Query: 508 AVMEDRHYCGKCHSTM 555
A +DR YCG+CH T+
Sbjct: 131 ANHKDRLYCGRCHLTL 146
>SPAC589.10c |||ribomal-ubiquitin fusion protein
Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 168 bits (408), Expect = 1e-42
Identities = 83/136 (61%), Positives = 96/136 (70%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXX 327
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 13 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 72
Query: 328 XXXXXXXXXXXNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFM 507
Y+TP LAVL++YKV+++G + RLRREC CGA FM
Sbjct: 73 LRGGGKKRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFM 130
Query: 508 AVMEDRHYCGKCHSTM 555
A +DR YCG+CH T+
Sbjct: 131 ANHKDRLYCGRCHLTL 146
>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 108 bits (259), Expect = 1e-24
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 13 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70
>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 108 bits (259), Expect = 1e-24
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 13 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70
>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 382
Score = 108 bits (259), Expect = 1e-24
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 13 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70
Score = 108 bits (259), Expect = 1e-24
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 89 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 146
Score = 108 bits (259), Expect = 1e-24
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 165 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 222
Score = 108 bits (259), Expect = 1e-24
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 241 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 298
Score = 108 bits (259), Expect = 1e-24
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
ITLEVE S TI+ VK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 317 ITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 374
>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
protein modifier Ned8|Schizosaccharomyces pombe|chr
2|||Manual
Length = 78
Score = 67.7 bits (158), Expect = 2e-12
Identities = 26/58 (44%), Positives = 43/58 (74%)
Frame = +1
Query: 148 ITLEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
I L+++P+ + +K ++++KEGIPP+QQRLI+AGKQ+ D + Y+++ S LHLV
Sbjct: 13 IELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLV 70
>SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 368
Score = 39.1 bits (87), Expect = 8e-04
Identities = 16/49 (32%), Positives = 31/49 (63%)
Frame = +1
Query: 157 EVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKE 303
+V I +K KIQ ++ +Q+LI++G+ L D +T+ +YNI+++
Sbjct: 17 DVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQ 65
>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = +1
Query: 154 LEVEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTL 312
LE+EP+ T +K ++ +PP +Q++I G QL+D L I+ +TL
Sbjct: 15 LEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67
>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 354
Score = 37.9 bits (84), Expect = 0.002
Identities = 19/54 (35%), Positives = 30/54 (55%)
Frame = +1
Query: 160 VEPSXTIEXVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 321
V+ ++ +K I I +QRLI+AG+ L+D +L Y IQ ++HLV
Sbjct: 20 VDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLV 73
>SPAC227.15 |||protein phosphatase regulatory subunit Reg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 873
Score = 30.7 bits (66), Expect = 0.30
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -1
Query: 633 NPSYTAVASVSYRLEGTYLFVVVFEDHRAVTLPAV 529
NP+YT SY L TYL F++HR + AV
Sbjct: 144 NPAYTNSHQNSYSLNETYLSYDFFDNHRGASSSAV 178
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = -3
Query: 301 PSGYCSPRGYVHLPAAFQQRSTSVDWEGF 215
PS + PRG V+ P+ ++R + + GF
Sbjct: 17 PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45
>SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 26.6 bits (56), Expect = 4.8
Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = +1
Query: 415 VLRFY-KVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 573
V+R Y ++ +N + R C E F V++D H KC + ++ D
Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,341,498
Number of Sequences: 5004
Number of extensions: 39687
Number of successful extensions: 91
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 89
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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