Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_D14
(897 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 301 8e-83
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 294 1e-80
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 154 1e-38
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 133 4e-32
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 30 0.39
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.4
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.4
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 301 bits (739), Expect = 8e-83
Identities = 132/192 (68%), Positives = 159/192 (82%)
Frame = +2
Query: 152 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 331
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 332 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 511
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 512 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 691
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 692 VVEPYNSILTTH 727
VVEPYNS+LTTH
Sbjct: 181 VVEPYNSVLTTH 192
Score = 77.8 bits (183), Expect = 2e-15
Identities = 34/44 (77%), Positives = 38/44 (86%)
Frame = +3
Query: 744 SDCAFMVDNEAIYDICRRNLDIERPTYXNLNRLIGXIVSSITXS 875
+DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI +VSSIT S
Sbjct: 198 ADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITAS 241
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 294 bits (721), Expect = 1e-80
Identities = 133/197 (67%), Positives = 161/197 (81%), Gaps = 5/197 (2%)
Frame = +2
Query: 152 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 316
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 317 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 496
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 497 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 676
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 677 QVSTAVVEPYNSILTTH 727
QVST+VVEPYNS+LTTH
Sbjct: 180 QVSTSVVEPYNSVLTTH 196
Score = 83.0 bits (196), Expect = 5e-17
Identities = 37/46 (80%), Positives = 40/46 (86%)
Frame = +3
Query: 738 EHSDCAFMVDNEAIYDICRRNLDIERPTYXNLNRLIGXIVSSITXS 875
++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI +VSSIT S
Sbjct: 200 DNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITAS 245
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 154 bits (374), Expect = 1e-38
Identities = 75/192 (39%), Positives = 107/192 (55%)
Frame = +2
Query: 152 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 331
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 332 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 511
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 512 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 691
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 692 VVEPYNSILTTH 727
VVEPYN+ L+ H
Sbjct: 179 VVEPYNATLSMH 190
Score = 45.2 bits (102), Expect = 1e-05
Identities = 19/47 (40%), Positives = 30/47 (63%)
Frame = +3
Query: 738 EHSDCAFMVDNEAIYDICRRNLDIERPTYXNLNRLIGXIVSSITXSF 878
E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T SF
Sbjct: 194 ENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSF 240
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 133 bits (321), Expect = 4e-32
Identities = 72/191 (37%), Positives = 110/191 (57%), Gaps = 3/191 (1%)
Frame = +2
Query: 155 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 334
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 335 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 508
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 509 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 685
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 686 TAVVEPYNSIL 718
VV+PYNS+L
Sbjct: 180 DVVVQPYNSLL 190
Score = 29.9 bits (64), Expect = 0.51
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = +3
Query: 741 HSDCAFMVDNEAIYDICRRNLDIERPTYXNLNRLIGXIVSSIT 869
++D ++DN A+ I L + PT+ N+L+ ++S+ T
Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAST 240
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 30.3 bits (65), Expect = 0.39
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = -2
Query: 896 RAPSNXKRXSDRGDNXSDETIQVGVGWALNVEITAADVIDG 774
R+ SN K+ S D ++T VG+G AL I + D +DG
Sbjct: 177 RSNSNDKQKSSDNDEDPNDTT-VGIGAALAASILSVDSVDG 216
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 231 STASSLMARCPQTRPSGVETILSTLSSARPELAST 335
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 250 WPDAHRQDHRGWRRFFQHFLQR 315
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,527,746
Number of Sequences: 5004
Number of extensions: 72286
Number of successful extensions: 215
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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