Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP27_F_A21
(884 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 379 e-106
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 213 3e-56
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 210 2e-55
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 161 2e-40
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 97 2e-21
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 89 1e-18
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 79 1e-15
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 67 3e-12
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 67 4e-12
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.002
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.38
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 4.7
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.2
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 6.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 379 bits (932), Expect = e-106
Identities = 176/197 (89%), Positives = 187/197 (94%)
Frame = +3
Query: 246 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 425
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 426 VLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHT 605
LLTEAPLNPK+NREKMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HT
Sbjct: 103 CLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHT 162
Query: 606 VPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEKLCYVALD 785
VPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTA REIVRDIKEKLCYVALD
Sbjct: 163 VPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALD 222
Query: 786 FEQEMATXXXSTFLEKS 836
FEQE+ T S+ LEKS
Sbjct: 223 FEQELQTAAQSSSLEKS 239
Score = 84.2 bits (199), Expect = 2e-17
Identities = 35/41 (85%), Positives = 40/41 (97%)
Frame = +2
Query: 122 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 244
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
Score = 33.1 bits (72), Expect = 0.054
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = +2
Query: 836 YELPDGQVITIGXEXF 883
YELPDGQVITIG E F
Sbjct: 240 YELPDGQVITIGNERF 255
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 213 bits (520), Expect = 3e-56
Identities = 96/190 (50%), Positives = 137/190 (72%)
Frame = +3
Query: 267 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 446
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 447 LNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGY 626
+NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169
Query: 627 ALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEKLCYVALDFEQEMAT 806
L H + RLD+AGRD T YL+ +L +GY+F TA E VR++KEKLCYV+ D E +
Sbjct: 170 VLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKL 229
Query: 807 XXXSTFLEKS 836
+T L ++
Sbjct: 230 SEETTVLMRN 239
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +2
Query: 134 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 232
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 210 bits (513), Expect = 2e-55
Identities = 97/186 (52%), Positives = 135/186 (72%), Gaps = 1/186 (0%)
Frame = +3
Query: 246 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEH 422
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + ++ L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 423 PVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 602
P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166
Query: 603 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEKLCYVAL 782
+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++A +EIVR+IKEK CYVA
Sbjct: 167 SVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVAS 226
Query: 783 DFEQEM 800
DF E+
Sbjct: 227 DFRSEI 232
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +2
Query: 128 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 241
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
Score = 29.1 bits (62), Expect = 0.88
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +2
Query: 776 RPRLRAGDGHRXRLHLPREVYELPDGQVITIGXEXF 883
R + + H+ ++H Y+LPD Q IT+G E F
Sbjct: 229 RSEIESWTEHKPQIH----TYQLPDNQTITLGTECF 260
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 161 bits (390), Expect = 2e-40
Identities = 88/218 (40%), Positives = 127/218 (58%), Gaps = 12/218 (5%)
Frame = +3
Query: 186 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 353
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 354 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQA 533
WD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+FN +Y+A+QA
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQA 161
Query: 534 VLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLM 689
VL+L AS + TG V+DSGDGV+H +P+ EGY + +I + LAGRD+T ++
Sbjct: 162 VLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQ 221
Query: 690 KILTERGYSFTTTAXREIVRDIKEKLCYVALDFEQEMA 803
+L +R ++ E IKE+ CYV D +E +
Sbjct: 222 SLLRDRNEPDSSLKTAE---RIKEECCYVCPDIVKEFS 256
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +2
Query: 140 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 223
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 97.5 bits (232), Expect = 2e-21
Identities = 53/195 (27%), Positives = 100/195 (51%), Gaps = 3/195 (1%)
Frame = +3
Query: 258 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 434
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 435 TEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTV 608
TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH +
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180
Query: 609 PIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEKLCYVALDF 788
P+ G + R+ G + YL+K+ + SF + C+V+ D+
Sbjct: 181 PVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDY 240
Query: 789 EQEMATXXXSTFLEK 833
++A LE+
Sbjct: 241 THDIAHALDRDILER 255
Score = 37.5 bits (83), Expect = 0.003
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +2
Query: 62 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 235
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 88.6 bits (210), Expect = 1e-18
Identities = 43/134 (32%), Positives = 69/134 (51%)
Frame = +3
Query: 312 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 491
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 492 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 671
E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNA 182
Query: 672 LTDYLMKILTERGY 713
+ D L + L ++ +
Sbjct: 183 INDILAQTLRDKNF 196
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +2
Query: 125 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 298
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 78.6 bits (185), Expect = 1e-15
Identities = 41/153 (26%), Positives = 77/153 (50%), Gaps = 2/153 (1%)
Frame = +3
Query: 267 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 440
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 441 APLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYE 620
NP++ R+++ + FE + PA Y+ QAV +A+ ++T +++D G + P+ +
Sbjct: 109 PSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVD 168
Query: 621 GYALPHAILRLDLAGRDLTDYLMKILTERGYSF 719
G + I + LAG L + ++ F
Sbjct: 169 GLIIRKGIFKQSLAGDFLNANIEQLFNTMNIEF 201
Score = 34.3 bits (75), Expect = 0.023
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +2
Query: 125 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 226
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 67.3 bits (157), Expect = 3e-12
Identities = 48/193 (24%), Positives = 89/193 (46%), Gaps = 18/193 (9%)
Frame = +3
Query: 276 YVGDEAQSKRGILTLKYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEA 443
++G+E + TL++ H G + +W +W N + P ++ +LLT+
Sbjct: 43 FLGNELANCNDFTTLQFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQP 102
Query: 444 PLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG--------------IVLD 581
+ Q++FE F A A L + G T +V+D
Sbjct: 103 VFTMPSIEHNTIQLVFEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVID 162
Query: 582 SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKE 761
SG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ IV +IKE
Sbjct: 163 SGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNMMEETY--IVNEIKE 220
Query: 762 KLCYVALDFEQEM 800
+CYV+ +F+++M
Sbjct: 221 SVCYVSQNFKEDM 233
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 66.9 bits (156), Expect = 4e-12
Identities = 40/130 (30%), Positives = 63/130 (48%), Gaps = 4/130 (3%)
Frame = +3
Query: 327 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 494
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 495 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 674
P +A + ++ LYA G G+V+D G + PI +G + A +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231
Query: 675 TDYLMKILTE 704
T +L +L E
Sbjct: 232 TQHLQNLLRE 241
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 37.9 bits (84), Expect = 0.002
Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%)
Frame = +3
Query: 318 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 461
L YPI+ G+ D D+ +I+ ++ + L++ + + V+ L +
Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257
Query: 462 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 641
EK+ I+F + + +++ + + +G + V+D G + + EG +P++
Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317
Query: 642 ILRLDLAGRDLTDYLMKILTERGYSF 719
++++ G D+T MK+L + +
Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.38
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +3
Query: 387 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 530
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 4.7
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +1
Query: 613 STKVTLCPTPSSVWTW 660
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -1
Query: 350 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 237
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 6.2
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +3
Query: 429 LLTEAPLNPKANREKMTQIMFETFN 503
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,129,965
Number of Sequences: 5004
Number of extensions: 62466
Number of successful extensions: 244
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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