SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP26_F_P03
         (898 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC31A2.08 |mrp20||mitochondrial ribosomal protein subunit L23|...    44   4e-05
SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein L25|S...    36   0.008
SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosacch...    32   0.096
SPBC16G5.17 |||transcription factor, zf-fungal binuclear cluster...    27   4.8  
SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomy...    26   6.3  
SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr...    26   8.4  
SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||...    26   8.4  
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos...    26   8.4  

>SPAC31A2.08 |mrp20||mitochondrial ribosomal protein subunit
           L23|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 161

 Score = 43.6 bits (98), Expect = 4e-05
 Identities = 26/91 (28%), Positives = 46/91 (50%)
 Frame = +2

Query: 170 VFLPNFWMKLVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFK 349
           V+ PN    L R    Q P    F   + + K+D+++YL+ +Y + VV V+++I  GK  
Sbjct: 10  VYFPNITFTLCRGLNLQ-PKFAVFRVPLHVNKFDVRDYLQHVYNLKVVSVKSRIYQGKLF 68

Query: 350 KDVVKGYVIKEDDVKVAFVTLPKTMTFKYPD 442
           ++ + G V +    K   V + +   F YP+
Sbjct: 69  RNQL-GQVKRPQSEKRVIVEMEE--PFVYPN 96


>SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein
           L25|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 141

 Score = 35.9 bits (79), Expect = 0.008
 Identities = 17/46 (36%), Positives = 25/46 (54%)
 Frame = +2

Query: 227 NIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVK 364
           N + FH  ++  K+ IKN ++K+Y V  V + T I     KK  VK
Sbjct: 79  NTLVFHVHLKANKFTIKNAVKKLYSVDAVKINTLIRPNGTKKAFVK 124


>SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein
           L25|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 141

 Score = 32.3 bits (70), Expect = 0.096
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = +2

Query: 227 NIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVK 364
           N + FH  ++  K+ IK  + K+Y V  V + T I     KK  VK
Sbjct: 79  NTLVFHVHLKANKFTIKEAVRKLYSVEPVKINTLIRPNGTKKAFVK 124


>SPBC16G5.17 |||transcription factor, zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 560

 Score = 26.6 bits (56), Expect = 4.8
 Identities = 11/40 (27%), Positives = 21/40 (52%)
 Frame = +2

Query: 401 FVTLPKTMTFKYPDLFEKSINEEEEHAKSLDESKKNFRKY 520
           F+T+ +T+   YPD+ +K      + ++ +  S   F KY
Sbjct: 418 FITVSRTLVPNYPDIVQKLGQTSIQLSRLISNSMDCFEKY 457


>SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 735

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 13/45 (28%), Positives = 21/45 (46%)
 Frame = +2

Query: 410 LPKTMTFKYPDLFEKSINEEEEHAKSLDESKKNFRKYIDHNKSRP 544
           +PK  T +  +  EK   EEEE  + L+   +   + +DH    P
Sbjct: 1   MPKNRTGRSREAREKKRKEEEEEIEELNSQIEALSETVDHFAELP 45


>SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 359

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 3/31 (9%)
 Frame = +2

Query: 437 PDLFEKSINEEEEHAKSLDESKKNF---RKY 520
           PDL E+S  +EE  + S  E K NF   RKY
Sbjct: 141 PDLKEESSTDEEMDSLSAAEEKPNFLQKRKY 171


>SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1101

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 10/36 (27%), Positives = 22/36 (61%)
 Frame = +2

Query: 473 EHAKSLDESKKNFRKYIDHNKSRPDIPSWFSI*SFI 580
           E+ +++D   ++F+K +  NKS+P   S+  +  F+
Sbjct: 569 ENVRTMDRFYQSFQKALSMNKSQPSCLSFSKLNDFV 604


>SPAC694.06c |mrc1||mediator of replication checkpoint 1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1019

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 18/84 (21%), Positives = 37/84 (44%), Gaps = 7/84 (8%)
 Frame = +2

Query: 248 SMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIK-------EDDVKVAFV 406
           S+E    ++K    +  ++    + T +N  + KK+  K +++K       EDD+     
Sbjct: 297 SVEDDSLELKEITPEALDIGQTSLFTTLNQTQVKKEDNKKFLLKEINAKLNEDDIDSELE 356

Query: 407 TLPKTMTFKYPDLFEKSINEEEEH 478
              K  T    ++ +  ++EE EH
Sbjct: 357 IEVKPKTTALDNIEKSKLSEENEH 380


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,257,611
Number of Sequences: 5004
Number of extensions: 40490
Number of successful extensions: 135
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -