BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP26_F_F06
(916 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 4.9
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.9
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 4.9
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 6.5
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 8.6
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.6
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 8.6
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1
Query: 442 LGRALDVLPRLFQSLLGLAVRVSERXPGDSW 350
+G + L RLF SL GL ++ PG+ W
Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 187 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 306
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 181 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 270
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 6.5
Identities = 19/64 (29%), Positives = 29/64 (45%)
Frame = +1
Query: 94 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 273
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 274 SKDA 285
SKDA
Sbjct: 230 SKDA 233
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 8.6
Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
Frame = +3
Query: 552 KVSSNVQET--NEKLAPKIKAAYD 617
+V N++ET EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 151 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 327
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 328 LQQL 339
Q+
Sbjct: 73 NNQI 76
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 25.8 bits (54), Expect = 8.6
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -3
Query: 419 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 297
AST+ K PWP + L +P + + CS+ +E ++ +
Sbjct: 38 ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,303,009
Number of Sequences: 5004
Number of extensions: 36784
Number of successful extensions: 145
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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