Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP26_F_D05
(880 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 32 0.094
SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang... 30 0.38
SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharo... 29 0.87
SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S... 29 1.2
SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imida... 28 2.0
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 2.7
SPAC1565.04c |ste4||adaptor protein Ste4|Schizosaccharomyces pom... 27 2.7
SPCC417.15 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 27 4.7
SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase Rdh54... 27 4.7
SPAPB2B4.07 |||ubiquitin family protein, human UBTD1 homolog|Sch... 26 6.1
SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 26 6.1
SPAC19D5.02c |||peroxisomal membrane protein Pex22 |Schizosaccha... 26 8.1
SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6 |Schi... 26 8.1
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex
subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 32.3 bits (70), Expect = 0.094
Identities = 25/103 (24%), Positives = 44/103 (42%), Gaps = 1/103 (0%)
Frame = +1
Query: 385 IYVASNDGIYVYKTGDKSFKKYGTFKADVISLTKMNGSDLFYAVTNDNKAYKVTENGNKY 564
++ + +DGI++ +F++ F+ + +L + G D T N T G
Sbjct: 379 LFPSGSDGIHLVVDEKSNFREEN-FQRAMSALMEKQGDDPAAMATKGNAKKGKTGKGGVK 437
Query: 565 VLDDNLKAVKQVMFDNFN-VLHYVTLDNEVFKVKETMEKIDFN 690
D K VK +M N+N V+ + E + M K+D N
Sbjct: 438 GPSDIYKIVKMIMVKNYNPVIVFSFSKRECEALALQMSKLDMN 480
>SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange
factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 30.3 bits (65), Expect = 0.38
Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = +1
Query: 514 VTNDNKAYKVTENG--NKYVLDDNLKAVKQVMFDNFNVLHYVTLDNEVFKV-KETMEKID 684
V N N +K+T+ N +D+ + + +DN +V LD+E+ + KE EK +
Sbjct: 466 VFNSNNKHKMTKQEFINLCDIDELAPEIMEYYYDNITFTPFVNLDDELLLIEKEKNEKYN 525
Query: 685 F 687
F
Sbjct: 526 F 526
>SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1205
Score = 29.1 bits (62), Expect = 0.87
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +1
Query: 439 FKKYGTFKADVISLTKMNGSDLFYAVTNDNKAYKVTENGNK--YVLDDNLKAVKQVMFDN 612
F Y F D + + N +F+ + ++Y V E+GN YVL D + + QV N
Sbjct: 1001 FIAYIRFYVDTV-VNSENVPIVFHLMQRIKQSYDVIEDGNNYIYVLSDMAQKILQVKSQN 1059
Query: 613 F 615
F
Sbjct: 1060 F 1060
>SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor
Raf2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 28.7 bits (61), Expect = 1.2
Identities = 19/58 (32%), Positives = 27/58 (46%), Gaps = 6/58 (10%)
Frame = -2
Query: 666 LFHFKYFIIQSDI----VQYIEVIKHNL--FDGFQIIIQNVFVTIFSDFVSFIVVCYG 511
LFHF F I S + Y + I H+ F + I + FVT+ DFV + +G
Sbjct: 417 LFHFLLFDIGSGLSGSDYTYEQYINHSAVAFSFTEEIFEKNFVTVLPDFVKLFSISFG 474
>SPBC11B10.02c |his3||histidinol-phosphate aminotransferase
imidazole acetol phosphate transaminase
His3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +1
Query: 253 IYTIHADINDNRIEKGVYEVDLNLN 327
+YT+ A IND + K + E D NLN
Sbjct: 117 MYTVSAKINDVEVVKVLLEPDFNLN 141
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/50 (34%), Positives = 28/50 (56%)
Frame = -3
Query: 194 VEYSSTLRCLKTEPLSTLEAVTLSPTTKMRRVAAFILDIQVSFSENVQQN 45
VE +++L ++ L+T A+ L +RR + +I+ SFSE QQN
Sbjct: 855 VETNNSLTAVENL-LTTERAIALDKEEILRRTQERLANIEDSFSETKQQN 903
>SPAC1565.04c |ste4||adaptor protein Ste4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 264
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/60 (28%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = +1
Query: 520 NDNKAYKVTENGNKYVLDDNLKAVKQVMF-DNFNVLHYVTLDNEVFKVKETMEKIDFNVK 696
N ++YK E +++ DDN+ K+V + + N TL++ + + + KI NVK
Sbjct: 89 NIEESYKKLEEKTEHLSDDNVSLEKRVEYLETENTKLVKTLNSLNSEFLQLLRKIAINVK 148
>SPCC417.15 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
Length = 43
Score = 26.6 bits (56), Expect = 4.7
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -1
Query: 421 YIHISRHC*LRISCCHHHNINPFLC 347
+IH S HC C +H INP +C
Sbjct: 18 HIHFSHHC------CENHFINPLVC 36
>SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase
Rdh54|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 26.6 bits (56), Expect = 4.7
Identities = 22/95 (23%), Positives = 43/95 (45%), Gaps = 4/95 (4%)
Frame = +1
Query: 442 KKYGTFKADVISLTKMNGSDLFYAVTNDNKAYKVTENGNKYV----LDDNLKAVKQVMFD 609
K+ TF+ +I T + S + +++V+EN N++ +D N A V++
Sbjct: 2 KRRATFQCPLIESTIKHQSTNYTKTETATTSHEVSENANEHKGKSNIDINKIAYYNVVWR 61
Query: 610 NFNVLHYVTLDNEVFKVKETMEKIDFNVKIDYVRL 714
+ + T + + F V+E +N D+ RL
Sbjct: 62 KITMKKHKTWEGDGFLVREDSNLTLYN--SDFTRL 94
>SPAPB2B4.07 |||ubiquitin family protein, human UBTD1
homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 239
Score = 26.2 bits (55), Expect = 6.1
Identities = 30/134 (22%), Positives = 50/134 (37%), Gaps = 6/134 (4%)
Frame = +1
Query: 175 SVELYSTEDQVVGAFVISNAEKSDGKIYTIHADINDNRIEKGVYEVDLNLNTTVKILDK- 351
S E++ +VV NAE + I +N I KGVY+ KI+ +
Sbjct: 61 SKEIWDVLHKVVTLLYEGNAEAATEMALAADLTIPENDISKGVYDSKGTFYEIPKIVARI 120
Query: 352 -----GRDLCYDDDSTIYVASNDGIYVYKTGDKSFKKYGTFKADVISLTKMNGSDLFYAV 516
R DD+ ++SND + D + K + K + + +
Sbjct: 121 PRAFAERKDSLDDEDDNMISSNDPTKSPEEHDTTTKSIASLKDAELDSSL---ETVLIRY 177
Query: 517 TNDNKAYKVTENGN 558
+ D+K Y + N N
Sbjct: 178 SKDDKDYSIQINPN 191
>SPAC328.04 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -3
Query: 317 KSTSYTPFSIRLSLISACIVYILPSLFSALDITKAPT 207
++T S +LS S I + PSL S +TK+P+
Sbjct: 303 RTTEVPSISKQLSKSSTSIATVAPSLASVSSVTKSPS 339
>SPAC19D5.02c |||peroxisomal membrane protein Pex22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 223
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/73 (20%), Positives = 34/73 (46%)
Frame = +1
Query: 472 ISLTKMNGSDLFYAVTNDNKAYKVTENGNKYVLDDNLKAVKQVMFDNFNVLHYVTLDNEV 651
I + ++ SD ++ +KY +D+ +K + +V D++ +L + NE+
Sbjct: 54 IDWSNLSHSDFVQCGVYEDSTNTWLAGASKYKIDE-IKTLPKVPRDHYIILCDSSESNEI 112
Query: 652 FKVKETMEKIDFN 690
K + + DF+
Sbjct: 113 AKFTQVVHSFDFS 125
>SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 624
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/48 (22%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = -2
Query: 696 FNIKIDFFHSLFHFKYFIIQSDIVQYIEVIKHNLFDGFQII-IQNVFV 556
FN +DF +F + +++ +EV+K+ L + + ++ + N++V
Sbjct: 472 FNQDLDFDEKMFSRQLSLVKGQAYNIVEVLKNPLMNIYDLLELPNLYV 519
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,388,043
Number of Sequences: 5004
Number of extensions: 67504
Number of successful extensions: 200
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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