Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP26_F_C11
(876 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 55 1e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 44 4e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 43 5e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.008
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr... 29 0.66
SPAC3A11.07 |||NADH dehydrogenase|Schizosaccharomyces pombe|chr ... 29 0.66
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.0
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 3.5
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ... 26 8.1
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 26 8.1
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 8.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 55.2 bits (127), Expect = 1e-08
Identities = 29/78 (37%), Positives = 43/78 (55%)
Frame = +3
Query: 321 TAKQRKTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCM 500
T RK K E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA- 130
Query: 501 DPEDDDGMIPYAAFLKKV 554
D DG+I Y F + +
Sbjct: 131 -DTDGDGVINYEEFSRVI 147
Score = 31.1 bits (67), Expect = 0.22
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +2
Query: 110 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI 253
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAEL 50
Score = 29.1 bits (62), Expect = 0.87
Identities = 15/61 (24%), Positives = 32/61 (52%)
Frame = +3
Query: 363 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 542
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 543 L 545
L
Sbjct: 71 L 71
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 43.6 bits (98), Expect = 4e-05
Identities = 22/67 (32%), Positives = 41/67 (61%)
Frame = +3
Query: 360 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 539
E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+ Y
Sbjct: 77 EEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVNYHD 133
Query: 540 FLKKVMA 560
F++ ++A
Sbjct: 134 FVQMILA 140
Score = 42.3 bits (95), Expect = 9e-05
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = +2
Query: 152 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI 253
AFS++D G G+I ++GDLLRA NPTLA I
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI 44
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 43.2 bits (97), Expect = 5e-05
Identities = 21/75 (28%), Positives = 44/75 (58%)
Frame = +3
Query: 336 KTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 515
K + + E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP +
Sbjct: 70 KLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-TN 127
Query: 516 DGMIPYAAFLKKVMA 560
G Y F++++MA
Sbjct: 128 SGSFDYYDFVQRIMA 142
Score = 30.7 bits (66), Expect = 0.28
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = +3
Query: 360 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 539
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.9 bits (79), Expect = 0.008
Identities = 21/74 (28%), Positives = 37/74 (50%)
Frame = +3
Query: 273 RRRARSCSHSKSSFPSTAKQRKTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALG 452
+RR+R+ S + + A R T +D E KL+D +++ + EL + ALG
Sbjct: 8 KRRSRASSPTPARLGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
Query: 453 EKLDDSEVAEVTKD 494
+ SEV ++ +D
Sbjct: 68 FNAEKSEVLKILRD 81
Score = 27.9 bits (59), Expect = 2.0
Identities = 19/74 (25%), Positives = 31/74 (41%)
Frame = +3
Query: 336 KTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 515
K R E+ +L+D +E G + L L E +DD E+ + ++ D
Sbjct: 102 KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLDQ 159
Query: 516 DGMIPYAAFLKKVM 557
DG I F+ +M
Sbjct: 160 DGEINEQEFIAIMM 173
Score = 26.6 bits (56), Expect = 4.6
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +2
Query: 119 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 235
+ ++ +E AF ++D + GKI NL + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 178
Score = 29.5 bits (63), Expect = 0.66
Identities = 27/107 (25%), Positives = 48/107 (44%), Gaps = 3/107 (2%)
Frame = +3
Query: 84 EGTD-KHHTK*ATSARTTLKGRLSPSQSTTLKAKAR-SMPSTLAIS*ERSTQTPHWQPSR 257
E TD ++HT T A+ KG P++ KAK + ++ +S Q P++
Sbjct: 38 ERTDYRNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTK 97
Query: 258 NSVVQRRRARSCSHS-KSSFPSTAKQRKTKTRXAYEDFLECLKLYDK 395
+S+ + + + S K + S A+Q T+ + LK Y+K
Sbjct: 98 HSLDENEKDKENKKSKKETVSSPAEQLLALTQNQEISLYKLLKKYNK 144
>SPAC3A11.07 |||NADH dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 551
Score = 29.5 bits (63), Expect = 0.66
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +2
Query: 731 LPATSTSRIHLRYGQTPFLYEQHNINCFVQLSTFDCT 841
LP+T+T +H R P Y + +C+V+ +CT
Sbjct: 132 LPSTATGSVHTRSIVQPIRYMLRHKSCYVKFYEAECT 168
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -1
Query: 714 APPEELSPPRALPAPVPQSRASVF*GPSHRT 622
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 3.5
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +2
Query: 110 MSDLSKNDVER-ASFAFSIYDFEGKGKID 193
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 25.8 bits (54), Expect = 8.1
Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +3
Query: 273 RRRARSCSHSKSSFPSTAKQRKTKTRXAYE-DFLECLKLYDKNENGLM 413
RR+ S SH +SF + + +K R ++ D L+ L + + E+G++
Sbjct: 36 RRKHASHSHDDASFFDPSNEEASKLRESFAMDTLDALLQWFEEEDGVV 83
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.8 bits (54), Expect = 8.1
Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +3
Query: 129 TTLKGRLSPSQSTTLKAKAR-SMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 302
T +PS L+A S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 303 KSSFPSTAKQRKTKTRXAY 359
KSSFPST K + T Y
Sbjct: 369 KSSFPSTIKDQPNLTLLEY 387
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +3
Query: 447 LGEKLDDSEVAEVTKDCMDPED 512
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,139,306
Number of Sequences: 5004
Number of extensions: 57411
Number of successful extensions: 187
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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