Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP26_F_C01
(865 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 289 3e-79
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 282 5e-77
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 70 5e-13
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 48 1e-06
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 39 0.001
SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3... 32 0.12
SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 32 0.12
SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 27 3.4
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 26 6.0
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 26 7.9
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 289 bits (710), Expect = 3e-79
Identities = 129/245 (52%), Positives = 162/245 (66%)
Frame = +3
Query: 93 TVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGV 272
T GK+I C AAVAW+ PLSIE ++V PP+ EVR+KI +GVCHTDAYTLSGKDPEG+
Sbjct: 6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLSGKDPEGL 65
Query: 273 FPVVLXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQ 452
FPV+L T+V+ GD V+ LY P+C TCKFC + KTNLC ++R+TQG+
Sbjct: 66 FPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIRTTQGK 125
Query: 453 GVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVCLLGCGVPTGY 632
G+MPDGT RF C G L HFMGCSTFS+YTVV +IS+ + APLD VCLLGCG+ TGY
Sbjct: 126 GLMPDGTSRFSCNGNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSVCLLGCGITTGY 185
Query: 633 GAALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDINPDKFEVAKKFGVNEF 812
GAA TA ++ G + A+F RI G+D+NP+K A FG +F
Sbjct: 186 GAATITADIKEGDSVAVFGLGSVGLAVIQGAVKKRAGRIFGIDVNPEKKNWAMSFGATDF 245
Query: 813 VNPKD 827
+NP D
Sbjct: 246 INPND 250
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 282 bits (691), Expect = 5e-77
Identities = 131/244 (53%), Positives = 163/244 (66%)
Frame = +3
Query: 87 MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPE 266
MS GK I C AAVAW A +PLSIE+I+V PPKA EVRVK+ + VCHTDAYTLSG DPE
Sbjct: 1 MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCHTDAYTLSGVDPE 60
Query: 267 GVFPVVLXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQ 446
G FP+VL +V+PGDHV+ LY P+C CKFC + KTNLC K+R TQ
Sbjct: 61 GAFPIVLGHEGAGIVESIGEGVINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQ 120
Query: 447 GQGVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVCLLGCGVPT 626
G+G+MPDGT RF C+ + L H+MGCS+FSQYTVV +ISL ++ +APL +CLLGCGV T
Sbjct: 121 GRGLMPDGTSRFSCRDKTLLHYMGCSSFSQYTVVADISLVAISHSAPLRSICLLGCGVTT 180
Query: 627 GYGAALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDINPDKFEVAKKFGVN 806
G+GA ++AKVE GS A+ +RII +DIN DK AKKFG
Sbjct: 181 GFGAVTHSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIAIDINADKEVYAKKFGAT 240
Query: 807 EFVN 818
+F++
Sbjct: 241 DFID 244
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 69.7 bits (163), Expect = 5e-13
Identities = 66/242 (27%), Positives = 91/242 (37%), Gaps = 4/242 (1%)
Frame = +3
Query: 111 KCLAAVAWEAGKPLSI--EEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDP-EGVFPV 281
K LAAV G P ++ EE+ V P EV V I TGVCHTD + L G P P+
Sbjct: 6 KQLAAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQGDWPLPAKMPL 65
Query: 282 VLXXXXXXXXXXXXXXXTSVKPGDHV-VPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGV 458
+ T +K GD V V C C++C+ + +C + Q G
Sbjct: 66 IGGHEGAGVVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPHI---QLSGY 122
Query: 459 MPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVCLLGCGVPTGYGA 638
DG TF Y + + E+ PL+ + C T Y
Sbjct: 123 TVDG------------------TFQHYCIANATHATIIPESVPLEVAAPIMCAGITCY-R 163
Query: 639 ALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDINPDKFEVAKKFGVNEFVN 818
AL +KV PG I R++ +D DK E+ K FG F++
Sbjct: 164 ALKESKVGPGEWICIPGAGGGLGHLAVQYAKAMAMRVVAIDTGDDKAELVKSFGAEVFLD 223
Query: 819 PK 824
K
Sbjct: 224 FK 225
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 48.4 bits (110), Expect = 1e-06
Identities = 54/235 (22%), Positives = 91/235 (38%), Gaps = 7/235 (2%)
Frame = +3
Query: 117 LAAVAWEAGKPLSIEEIEVDPPKAG---EVRVKITATGVCH-TDAYTLSGKDPEGVFPVV 284
+ A W+ PL+++ EV P +V VK TA +C +D++ SG+ P +
Sbjct: 37 MKACVWDG--PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPGIEKGAI 94
Query: 285 LXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMP 464
L +++ GD VV + C C FC + C ++ V
Sbjct: 95 LGHESCGIVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDV-N 153
Query: 465 DGTRRFRCKG--QELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVCLLGCGVPTGYGA 638
G+ G + L GC EI+ CK+ + P D L V
Sbjct: 154 YGSHHSAIFGYTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIP-DSEGLFMSDVLCTSLH 212
Query: 639 ALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDINPDKFEVAK-KFG 800
A +V+ G AI+ +++IG+++ P++ E+A+ KFG
Sbjct: 213 ACTLGEVKKGDTVAIWGMGPIGLYAGRWAQILGASKVIGIEVVPERIELARQKFG 267
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 38.7 bits (86), Expect = 0.001
Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 3/81 (3%)
Frame = +3
Query: 192 EVRVKITATGVCHTDA-YTLSGKDPEGVF--PVVLXXXXXXXXXXXXXXXTSVKPGDHVV 362
+V+V I ATG+C +D Y G + + P++L +S+KPGD V
Sbjct: 31 QVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILGHESAGVVVEVGKGVSSLKPGDPVA 90
Query: 363 PLYVPQCNTCKFCLNPKTNLC 425
C C +C + + NLC
Sbjct: 91 VEPGCVCRLCDYCRSGRYNLC 111
>SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 260
Score = 31.9 bits (69), Expect = 0.12
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = +3
Query: 744 RIIGVDINPDKFEVAKKFGVNEFVNP 821
R++G+D +PD + A++ G+N +V P
Sbjct: 57 RVVGIDASPDMIKAARELGLNAYVIP 82
>SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 325
Score = 31.9 bits (69), Expect = 0.12
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +3
Query: 150 LSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGVFPVVL 287
+ I+ + + PK GE+ VKI A + +D +G P V+P ++
Sbjct: 23 IEIQSVPIPQPKNGELLVKIEAAAINPSDLMNATGGFPYTVYPRIV 68
>SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1250
Score = 27.1 bits (57), Expect = 3.4
Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Frame = +3
Query: 348 GDHVVPLY-VPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQEL 503
G HVV L V N C + KT KV + + P G+R++ C G L
Sbjct: 1036 GKHVVRLLQVRDANGCAASIT-KTQPAAKVSVVEMASLAPLGSRQYYCVGDRL 1087
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 26.2 bits (55), Expect = 6.0
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +3
Query: 561 LCKVAEAAPLDKVCLLGCGVPTGY 632
+CKV E A L CLL C T +
Sbjct: 979 ICKVREIASLSLTCLLDCSKMTTF 1002
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/47 (27%), Positives = 24/47 (51%)
Frame = -2
Query: 678 RNLILVQLWQYSRRLHNL*VHRIPANKLYLMEQPLQLYREKFLEQLC 538
R+L V + R H L +H++ K + +Q ++ R+ L+ LC
Sbjct: 210 RDLDAVACHERIARYHILCIHQLCEKKQFSAQQEVEQLRKGILQSLC 256
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,181,055
Number of Sequences: 5004
Number of extensions: 62038
Number of successful extensions: 153
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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