Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_P17
(888 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 79 1e-15
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 75 1e-14
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 68 2e-12
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 61 2e-10
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 57 3e-09
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 38 0.003
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 28 1.5
SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 28 2.0
SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomy... 27 3.6
SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 26 6.2
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 6.2
SPAPB17E12.10c |||SAM-dependent methyltransferase|Schizosaccharo... 26 8.2
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 78.6 bits (185), Expect = 1e-15
Identities = 32/65 (49%), Positives = 42/65 (64%)
Frame = +2
Query: 530 EVLTFTMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAGCTVVLKPAEQTPXXXXXXXXXX 709
E L + + P+GVCGQI+PWNYP+ M WKIAPALAAG +++K AE TP
Sbjct: 145 EKLAYAKRTPIGVCGQIVPWNYPLNMAGWKIAPALAAGNCIIIKSAETTPLSLLYFATLV 204
Query: 710 QRSGF 724
+ +GF
Sbjct: 205 EEAGF 209
Score = 39.5 bits (88), Expect = 6e-04
Identities = 19/35 (54%), Positives = 25/35 (71%)
Frame = +3
Query: 768 AGGALTHHPDVDKIAFTGSTEVGRIILGAASAVNL 872
AG + HP +DKIAFTGST+VG +I+ +A NL
Sbjct: 224 AGSYMAKHPGIDKIAFTGSTKVG-VIVQQLAASNL 257
Score = 31.9 bits (69), Expect = 0.13
Identities = 17/36 (47%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = +1
Query: 418 GKP-VKQSEEEVYFSASVLRYYAGKADKILGNTIPS 522
GKP V + +V + ++LRY AG ADKI G IP+
Sbjct: 107 GKPLVSNARGDVDGTIALLRYCAGWADKIYGQVIPT 142
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 74.9 bits (176), Expect = 1e-14
Identities = 30/46 (65%), Positives = 37/46 (80%)
Frame = +2
Query: 542 FTMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAGCTVVLKPAEQTP 679
+T EP+GVCGQIIPWN+P M +WKIAPA+A G T++LK AE TP
Sbjct: 155 YTRHEPIGVCGQIIPWNFPFLMCAWKIAPAVACGNTIILKTAELTP 200
Score = 68.5 bits (160), Expect = 1e-12
Identities = 34/95 (35%), Positives = 50/95 (52%)
Frame = +3
Query: 126 KVDVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRY 305
K ++ LFINN+ VD+V +P E +I +VA+ D+ D+D+AV A+ AF
Sbjct: 17 KTYIQPVGLFINNQHVDSVHGGRVKVYSPSTEKLICEVADADEEDVDIAVKVARAAFQTD 76
Query: 306 SPWRTMDASQRGXXXXXXXXXXXSQSRYLAELETL 410
+PWR ++QRG YLA +ETL
Sbjct: 77 APWRKFSSAQRGRCLSRLADCIEQNLEYLASIETL 111
Score = 46.8 bits (106), Expect = 4e-06
Identities = 19/34 (55%), Positives = 28/34 (82%)
Frame = +3
Query: 771 GGALTHHPDVDKIAFTGSTEVGRIILGAASAVNL 872
G A++ H D+DK+AFTGST VGR+++ AA++ NL
Sbjct: 231 GNAISSHMDIDKVAFTGSTGVGRMVMRAAASSNL 264
Score = 37.1 bits (82), Expect = 0.003
Identities = 20/54 (37%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
Frame = +1
Query: 418 GKPVKQSEEEVYFSASVLRYYAGKADKILGNTIPSE-RRSPYVHHE--GTCRSV 570
GK + + +V +A RYY G ADK G TI ++ +R Y HE G C +
Sbjct: 114 GKSITLARGDVQAAADCFRYYGGWADKDYGQTIETDIKRFAYTRHEPIGVCGQI 167
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 67.7 bits (158), Expect = 2e-12
Identities = 29/59 (49%), Positives = 40/59 (67%)
Frame = +2
Query: 545 TMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAGCTVVLKPAEQTPXXXXXXXXXXQRSG 721
T+K+PVGV G I PWN+P M++ K+ ALAAGCTVV++PA +TP +R+G
Sbjct: 155 TIKQPVGVVGIITPWNFPAAMITRKVGAALAAGCTVVIRPAAETPFTALALAKLAERAG 213
Score = 33.5 bits (73), Expect = 0.041
Identities = 29/118 (24%), Positives = 46/118 (38%)
Frame = +3
Query: 153 FINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYSPWRTMDAS 332
+I +WV A S KTF NP + V + + A+ A +AF Y R D
Sbjct: 27 YIGGKWVTAASGKTFDVENPGLNETLAPVTDMSVEETRKAIKVAHEAFLSY---RNSDIK 83
Query: 333 QRGXXXXXXXXXXXSQSRYLAELETLVLWKTGKTIRRRSILFRKRAQILRWKSGQDIR 506
+R + LA + TL + GK + A+ + W +G+ +R
Sbjct: 84 ERYAILRRWYDLIMENADDLATMMTL---ENGKALGDAKGEVVYAAKFIDWFAGEALR 138
Score = 31.5 bits (68), Expect = 0.17
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = +3
Query: 771 GGALTHHPDVDKIAFTGSTEVGRIILGAASA 863
G LT +P + K++FTGST VG+I+ +S+
Sbjct: 231 GIELTTNPLIRKVSFTGSTNVGKILAKQSSS 261
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 60.9 bits (141), Expect = 2e-10
Identities = 26/58 (44%), Positives = 35/58 (60%)
Frame = +2
Query: 506 EIPFLQNGEVLTFTMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAGCTVVLKPAEQTP 679
++P N F + P GV G I PWN+P+ M WK+ PA+A+G VVLKP+E P
Sbjct: 145 KVPLPNNPGFCAFEKRFPRGVIGVITPWNFPLKMALWKLVPAIASGNCVVLKPSELAP 202
Score = 39.1 bits (87), Expect = 8e-04
Identities = 26/89 (29%), Positives = 37/89 (41%), Gaps = 2/89 (2%)
Frame = +3
Query: 150 LFINNEWVDAV--SKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYSPWRTM 323
LFI+ ++V + + K P INP E +I A D+D AV A F R W
Sbjct: 23 LFIDGKFVSPIEPAAKPIPLINPATEEIIGTCANASAKDVDSAVENAYNTF-RSGIWAKW 81
Query: 324 DASQRGXXXXXXXXXXXSQSRYLAELETL 410
QRG + LA ++T+
Sbjct: 82 PGKQRGLVLRKIAKMMREKRELLAGIDTI 110
Score = 31.9 bits (69), Expect = 0.13
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +3
Query: 768 AGGALTHHPDVDKIAFTGSTEVGRIILGAAS 860
+G AL+ HP + +AFTGS G+ I+ AA+
Sbjct: 232 SGAALSCHPKIAYLAFTGSLATGKKIMHAAA 262
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 57.2 bits (132), Expect = 3e-09
Identities = 26/60 (43%), Positives = 35/60 (58%)
Frame = +2
Query: 545 TMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAGCTVVLKPAEQTPXXXXXXXXXXQRSGF 724
++K+PVGV I PWN+P M++ K ALAAGCT + PA +TP Q +GF
Sbjct: 150 SIKQPVGVSALITPWNFPAAMIARKGGAALAAGCTAIFLPAFRTPYVCLGLVRLAQEAGF 209
Score = 41.9 bits (94), Expect = 1e-04
Identities = 19/68 (27%), Positives = 35/68 (51%)
Frame = +3
Query: 132 DVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYSP 311
D + + F+ +W+ + + KTF NP +I +VA+ + A++AA +AF Y
Sbjct: 14 DKSHAQSFVQGKWISSPNNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAFKTYKN 73
Query: 312 WRTMDASQ 335
+ + SQ
Sbjct: 74 FTHVQRSQ 81
Score = 36.3 bits (80), Expect = 0.006
Identities = 15/31 (48%), Positives = 24/31 (77%)
Frame = +3
Query: 771 GGALTHHPDVDKIAFTGSTEVGRIILGAASA 863
G LT +P V K++FTGST VG+I++G +++
Sbjct: 226 GKELTTNPIVRKVSFTGSTNVGKILMGQSAS 256
Score = 33.9 bits (74), Expect = 0.031
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = +1
Query: 418 GKPVKQSEEEVYFSASVLRYYAGKADKILGNTIPSERRS 534
GKP+ Q+E EV + L++YA +A + G+ PS +S
Sbjct: 106 GKPLSQAEMEVTTCSGYLKWYAAEAVRTFGDVAPSSLQS 144
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 37.5 bits (83), Expect = 0.003
Identities = 17/41 (41%), Positives = 24/41 (58%)
Frame = +2
Query: 554 EPVGVCGQIIPWNYPIPMMSWKIAPALAAGCTVVLKPAEQT 676
EP+GV ++ WNYP+ I AL AG +V+K +E T
Sbjct: 126 EPLGVIAALVSWNYPLHNALGPIISALFAGNAIVVKGSELT 166
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/48 (33%), Positives = 25/48 (52%)
Frame = +3
Query: 6 FXYRSHYRESLRFWSCWRPTYCTLVDTLSDWIKRNIINMVKVDVKYTK 149
F Y S + SL + P+ +DT+S W++R I N K D + +K
Sbjct: 48 FRYYSFLKSSLENATLDGPSQVACLDTISVWLRR-IFNACKQDPEISK 94
>SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 649
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +3
Query: 153 FINNEWVDAVSKKTFPTINPQDETVITQVAEGDKAD 260
F+ D S+ TF TI PQ+ + E K+D
Sbjct: 224 FLQQSEADQPSEDTFTTIGPQNSLTVNSTIEKHKSD 259
>SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 27.1 bits (57), Expect = 3.6
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = -3
Query: 280 AATAKSISALSPSATCVMTVSSCGLMVGKVFLLTASTHSLLINSLVYLTST 128
+ +A +SA S + TC +S+ G LTA ++++ NS ST
Sbjct: 442 SGSATLVSASSATGTCASYLSAAGSSATNAISLTADSNAVSRNSSASTMST 492
>SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1052
Score = 26.2 bits (55), Expect = 6.2
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +1
Query: 565 SVRSDNTVELPHTNDVLENSTSLSCRMYR--CSEAS*TN 675
SVR +NT +L D++E + R++R SEA+ N
Sbjct: 357 SVRDENTKKLKELQDIIEEQSKHMARLHREGFSEAAENN 395
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.2 bits (55), Expect = 6.2
Identities = 16/35 (45%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 290 SIPPLFPMAYHGRFSTR-PPIA*ASRTSRIPI*IP 391
S PP P A GR + PP+ ASRTS P+ P
Sbjct: 386 SNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTP 420
>SPAPB17E12.10c |||SAM-dependent
methyltransferase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 301
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = +1
Query: 454 FSASVLRYYAGKADKILGNTIPSERRSPYVHHEGTCRSVRSDNTVELP 597
FS+ +LR+Y+ K NT+ S R+S +E + ++ + P
Sbjct: 28 FSSCLLRFYSNPPKKTKKNTLISLRKSAETANEKFIEKINKEHQLFKP 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,429,543
Number of Sequences: 5004
Number of extensions: 69641
Number of successful extensions: 219
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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