Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_N21
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC4B3.03c |||DUF21 domain protein|Schizosaccharomyces pombe|ch... 29 0.89
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 29 1.2
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 27 2.7
SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schiz... 27 3.6
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 27 4.7
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 4.7
SPCC663.15c |||conserved fungal protein|Schizosaccharomyces pomb... 27 4.7
SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M... 27 4.7
SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces... 26 8.3
SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p... 26 8.3
SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosac... 26 8.3
SPBC776.13 |cnd1||condensin subunit Cnd1|Schizosaccharomyces pom... 26 8.3
>SPCC4B3.03c |||DUF21 domain protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 679
Score = 29.1 bits (62), Expect = 0.89
Identities = 17/60 (28%), Positives = 26/60 (43%)
Frame = +3
Query: 534 AENSQEEDAATDRDNKSSDKTGAIEERLPKPDTGPETSGDCPPNEEDSGNIRTVHNDGDE 713
A SQ ++NKSS G + P T P + PN D + +T N+G++
Sbjct: 497 AHGSQRSSIDVSKENKSSVADGPSSQAHNGPSTQPP---EALPNGTDPSSSKTTENNGNQ 553
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 28.7 bits (61), Expect = 1.2
Identities = 19/71 (26%), Positives = 33/71 (46%)
Frame = +3
Query: 378 TGRDTPSNRERFKRRIADVEMAVSWVLKPQHRSRVMFQRFMPWWNAEDRRQLAENSQEED 557
+G ++ N + A+VE A W + Q+ +F F AE + + E+SQE
Sbjct: 357 SGTNSVFNASGERSESANVETAQVWDDREQYFFYEVFPNFNEGSIAEMKSSIYESSQEGI 416
Query: 558 AATDRDNKSSD 590
++ +NK D
Sbjct: 417 RSSSENNKKED 427
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 27.5 bits (58), Expect = 2.7
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 365 ARAGHWERYALESRKI*KENRRRRD 439
A H E+ A+ SRK+ KENR R+
Sbjct: 50 AAKNHGEKQAMGSRKLIKENREERE 74
>SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 526
Score = 27.1 bits (57), Expect = 3.6
Identities = 19/62 (30%), Positives = 27/62 (43%), Gaps = 3/62 (4%)
Frame = +3
Query: 537 ENSQEEDAATDRDNKSSDKTGA---IEERLPKPDTGPETSGDCPPNEEDSGNIRTVHNDG 707
E+ E +D DN+ D + I LP D T + N DS + T+H+ G
Sbjct: 465 EDGMEAVEPSDNDNEEEDFLNSQYLISANLPAVDMHSSTPVNEQSNPFDSFSSVTLHHAG 524
Query: 708 DE 713
DE
Sbjct: 525 DE 526
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 26.6 bits (56), Expect = 4.7
Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = +3
Query: 528 QLAENSQEED-AATDRDNKSSDKTGAIEERLPKPDTGPETSGDCPPNEEDSGNIRTVHND 704
+LA+ QE AT ++++SSD G + L T PE+S P +D+ ++ND
Sbjct: 187 ELAKQIQETSLGATTQEDESSD--GICWDELST--TSPESSKVSEPIIQDNTQTTHINND 242
Query: 705 GDETXCSTR 731
+ S+R
Sbjct: 243 SSDIRFSSR 251
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.6 bits (56), Expect = 4.7
Identities = 22/71 (30%), Positives = 29/71 (40%), Gaps = 5/71 (7%)
Frame = -2
Query: 509 PPRHEPLEHDPRA----VLRLQHPRHGHLDVGDSPFKSFAIRGRISPSDQPWPGGPRTP- 345
PP+ H P A V +Q P H + +P A RIS S P P TP
Sbjct: 928 PPKVGETYHPPTASGTRVPPVQQPSHPNPYTPVAPQSPVAAASRISSSPNMPPSNPYTPI 987
Query: 344 TLSSHAPSEAH 312
++S + AH
Sbjct: 988 AVASSTVNPAH 998
>SPCC663.15c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 657
Score = 26.6 bits (56), Expect = 4.7
Identities = 9/35 (25%), Positives = 21/35 (60%)
Frame = +3
Query: 513 AEDRRQLAENSQEEDAATDRDNKSSDKTGAIEERL 617
+EDR+ + N +E+DA + + + T ++++ L
Sbjct: 105 SEDRKPTSNNEEEDDADEAKSSNADSSTDSVQKSL 139
>SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1115
Score = 26.6 bits (56), Expect = 4.7
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 471 RSRVMFQRFMPWWNAEDRRQLAENSQEEDAAT 566
R R + +F+ W+ ++ QL E+ EDA T
Sbjct: 1024 RQRKLSLKFLAWYLLDEEIQLTEHDSIEDALT 1055
>SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 369
Score = 25.8 bits (54), Expect = 8.3
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = +2
Query: 317 PPKVHVMRVWAFAARQARAGHWERYALESRKI*KENRRRR 436
PP +MR A + + G+W ALE K +E R +R
Sbjct: 44 PPSKKLMRKLRKAGKIDKDGNWTPEALEEAKKKEEKRLKR 83
>SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 773
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -3
Query: 496 NLWNMTLERCCGFSTHDTAISTSAILLLNLSRFEGVSL 383
N ++ L R FST TA+S ++L L+ F+ + +
Sbjct: 28 NFSSLRLARAYTFSTRSTAVSQFSLLSLSQRSFQSLKI 65
>SPBC8D2.19 |mde3||serine/threonine protein kinase
Mde3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 559
Score = 25.8 bits (54), Expect = 8.3
Identities = 18/48 (37%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = -2
Query: 422 SPFKSFAIR-GRISPSDQPWPGGPRTPTLSSHAPSEAHRKMLPTLLSW 282
S K A R G + P +P+P P + P E H KM+P LL+W
Sbjct: 264 SQAKELAKRLGFMLPPTKPYPIQKLLP---QNCP-EGHAKMIPCLLAW 307
>SPBC776.13 |cnd1||condensin subunit Cnd1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1158
Score = 25.8 bits (54), Expect = 8.3
Identities = 14/36 (38%), Positives = 17/36 (47%)
Frame = +3
Query: 222 LRELALARTRCFA*STRGSDPAEESR*HFPMSLRRC 329
L +L LAR C A G D + S +FP S C
Sbjct: 710 LEDLVLARYTCIAIKRIGKDASGSSNINFPNSHTLC 745
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,266,944
Number of Sequences: 5004
Number of extensions: 65737
Number of successful extensions: 197
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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