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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP25_F_N18
         (927 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||...    31   0.23 
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    30   0.53 
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe...    28   2.1  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    26   6.6  
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac...    26   6.6  

>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1461

 Score = 31.1 bits (67), Expect = 0.23
 Identities = 18/59 (30%), Positives = 18/59 (30%)
 Frame = +1

Query: 415 PXTPPKXXXXPPXXXXXXXXXXXXXXXXXPPXKXPPPPXGXGGXFFXPPPPXPXXXXXG 591
           P  PP     P                  PP   PPPP G  G    PPPP P     G
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPP--PPPPPGVAGAGPPPPPPPPPAVSAG 788



 Score = 30.3 bits (65), Expect = 0.40
 Identities = 12/24 (50%), Positives = 12/24 (50%)
 Frame = +3

Query: 519 PPPXGXGGXFFXPPPPXPPXXXXG 590
           PPP G  G    PPPP PP    G
Sbjct: 765 PPPPGVAGAGPPPPPPPPPAVSAG 788



 Score = 27.5 bits (58), Expect = 2.8
 Identities = 12/29 (41%), Positives = 12/29 (41%)
 Frame = +1

Query: 505 PXKXPPPPXGXGGXFFXPPPPXPXXXXXG 591
           P   PPPP   G     PPPP P     G
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 29.9 bits (64), Expect = 0.53
 Identities = 17/60 (28%), Positives = 20/60 (33%), Gaps = 4/60 (6%)
 Frame = +2

Query: 701 PKGXPPPXXXGXGALFXXPP----PPXKXXKXXPPXGGGXPPXGXXXXPXXPPXXPPXXP 868
           P   PPP     G++   P     PP    +  P  G   PP         PP  PP  P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIP 396



 Score = 28.7 bits (61), Expect = 1.2
 Identities = 27/118 (22%), Positives = 28/118 (23%), Gaps = 1/118 (0%)
 Frame = +2

Query: 518 PPPXGGXGXFFLXPPPXPPXXXPXGXXXXXXXXXXXXXXXXXXXPPXXXXXPXXXXXXPX 697
           PP   G     L PPP PP     G                    P     P        
Sbjct: 325 PPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRA 384

Query: 698 XPK-GXPPPXXXGXGALFXXPPPPXKXXKXXPPXGGGXPPXGXXXXPXXPPXXPPXXP 868
                 PPP   G  A     PP     +   P     P       P  PP  PP  P
Sbjct: 385 VSNPPAPPPAIPGRSA--PALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLP 440



 Score = 26.2 bits (55), Expect = 6.6
 Identities = 10/30 (33%), Positives = 12/30 (40%)
 Frame = +2

Query: 755 PPPPXKXXKXXPPXGGGXPPXGXXXXPXXP 844
           PPPP +  +  PP G G         P  P
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343


>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 273

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 22/69 (31%), Positives = 22/69 (31%)
 Frame = -1

Query: 855 GXXGGXXGXXXXPXGGXPPPXGGXXFXFXXGGGGXXKRAPXPXXXGGGXPXGXXGXXXXX 676
           G  GG  G      GG  PP G   F    G GG           GGG P G  G     
Sbjct: 184 GHNGGGFGGFGGGSGG--PPPGPGGFGGFGGFGGEGHHHGGHGGFGGG-PGGFEGGPGGF 240

Query: 675 XGXXXXXGG 649
            G     GG
Sbjct: 241 GGGPGGFGG 249



 Score = 27.5 bits (58), Expect = 2.8
 Identities = 16/40 (40%), Positives = 16/40 (40%), Gaps = 2/40 (5%)
 Frame = -1

Query: 867 GXXGGXXGGXXGXXXXP--XGGXPPPXGGXXFXFXXGGGG 754
           G  GG  GG  G    P   GG P   GG    F  G GG
Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGG 260


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 12/27 (44%), Positives = 12/27 (44%), Gaps = 3/27 (11%)
 Frame = +1

Query: 502  PPXKXPPPPXG---XGGXFFXPPPPXP 573
            PP   PPPP       G    PPPP P
Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLP 1736


>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
           Spp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2363

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 11/24 (45%), Positives = 11/24 (45%)
 Frame = +1

Query: 502 PPXKXPPPPXGXGGXFFXPPPPXP 573
           PP   PPPP   G      PPP P
Sbjct: 5   PPGNPPPPPPPPGFEPPSQPPPPP 28



 Score = 26.2 bits (55), Expect = 6.6
 Identities = 10/19 (52%), Positives = 10/19 (52%)
 Frame = +1

Query: 517 PPPPXGXGGXFFXPPPPXP 573
           PPPP G       PPPP P
Sbjct: 12  PPPPPGFEPPSQPPPPPPP 30


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,822,340
Number of Sequences: 5004
Number of extensions: 24167
Number of successful extensions: 120
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 77
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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