BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_N18
(927 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.23
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.53
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 28 2.1
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.6
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 6.6
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.1 bits (67), Expect = 0.23
Identities = 18/59 (30%), Positives = 18/59 (30%)
Frame = +1
Query: 415 PXTPPKXXXXPPXXXXXXXXXXXXXXXXXPPXKXPPPPXGXGGXFFXPPPPXPXXXXXG 591
P PP P PP PPPP G G PPPP P G
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPP--PPPPPGVAGAGPPPPPPPPPAVSAG 788
Score = 30.3 bits (65), Expect = 0.40
Identities = 12/24 (50%), Positives = 12/24 (50%)
Frame = +3
Query: 519 PPPXGXGGXFFXPPPPXPPXXXXG 590
PPP G G PPPP PP G
Sbjct: 765 PPPPGVAGAGPPPPPPPPPAVSAG 788
Score = 27.5 bits (58), Expect = 2.8
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = +1
Query: 505 PXKXPPPPXGXGGXFFXPPPPXPXXXXXG 591
P PPPP G PPPP P G
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.9 bits (64), Expect = 0.53
Identities = 17/60 (28%), Positives = 20/60 (33%), Gaps = 4/60 (6%)
Frame = +2
Query: 701 PKGXPPPXXXGXGALFXXPP----PPXKXXKXXPPXGGGXPPXGXXXXPXXPPXXPPXXP 868
P PPP G++ P PP + P G PP PP PP P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIP 396
Score = 28.7 bits (61), Expect = 1.2
Identities = 27/118 (22%), Positives = 28/118 (23%), Gaps = 1/118 (0%)
Frame = +2
Query: 518 PPPXGGXGXFFLXPPPXPPXXXPXGXXXXXXXXXXXXXXXXXXXPPXXXXXPXXXXXXPX 697
PP G L PPP PP G P P
Sbjct: 325 PPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRA 384
Query: 698 XPK-GXPPPXXXGXGALFXXPPPPXKXXKXXPPXGGGXPPXGXXXXPXXPPXXPPXXP 868
PPP G A PP + P P P PP PP P
Sbjct: 385 VSNPPAPPPAIPGRSA--PALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLP 440
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/30 (33%), Positives = 12/30 (40%)
Frame = +2
Query: 755 PPPPXKXXKXXPPXGGGXPPXGXXXXPXXP 844
PPPP + + PP G G P P
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.9 bits (59), Expect = 2.1
Identities = 22/69 (31%), Positives = 22/69 (31%)
Frame = -1
Query: 855 GXXGGXXGXXXXPXGGXPPPXGGXXFXFXXGGGGXXKRAPXPXXXGGGXPXGXXGXXXXX 676
G GG G GG PP G F G GG GGG P G G
Sbjct: 184 GHNGGGFGGFGGGSGG--PPPGPGGFGGFGGFGGEGHHHGGHGGFGGG-PGGFEGGPGGF 240
Query: 675 XGXXXXXGG 649
G GG
Sbjct: 241 GGGPGGFGG 249
Score = 27.5 bits (58), Expect = 2.8
Identities = 16/40 (40%), Positives = 16/40 (40%), Gaps = 2/40 (5%)
Frame = -1
Query: 867 GXXGGXXGGXXGXXXXP--XGGXPPPXGGXXFXFXXGGGG 754
G GG GG G P GG P GG F G GG
Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGG 260
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 6.6
Identities = 12/27 (44%), Positives = 12/27 (44%), Gaps = 3/27 (11%)
Frame = +1
Query: 502 PPXKXPPPPXG---XGGXFFXPPPPXP 573
PP PPPP G PPPP P
Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 26.2 bits (55), Expect = 6.6
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +1
Query: 502 PPXKXPPPPXGXGGXFFXPPPPXP 573
PP PPPP G PPP P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPP 28
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +1
Query: 517 PPPPXGXGGXFFXPPPPXP 573
PPPP G PPPP P
Sbjct: 12 PPPPPGFEPPSQPPPPPPP 30
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,822,340
Number of Sequences: 5004
Number of extensions: 24167
Number of successful extensions: 120
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 77
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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