Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_N02
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 35 0.017
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 33 0.069
SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.64
SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schi... 29 0.84
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 29 1.1
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 28 1.5
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 28 2.0
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 27 2.6
SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ... 27 2.6
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 3.4
SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces p... 27 4.5
SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|c... 26 6.0
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 7.9
SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 26 7.9
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 34.7 bits (76), Expect = 0.017
Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 2/71 (2%)
Frame = +1
Query: 502 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSST--VIAPLT 675
VTP P S S PP +T T S P +++ S T + T +ST V +
Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVP 360
Query: 676 PSNTTGTLSPP 708
P++T+ T +PP
Sbjct: 361 PTSTSSTSTPP 371
Score = 33.5 bits (73), Expect = 0.039
Identities = 21/71 (29%), Positives = 35/71 (49%), Gaps = 2/71 (2%)
Frame = +1
Query: 502 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAP-LT 675
+TP P S S+P P T+ + S P T ST + + + S+T + P +
Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNSTTPVTPTVP 306
Query: 676 PSNTTGTLSPP 708
P++T+ T +PP
Sbjct: 307 PTSTSSTSTPP 317
Score = 32.3 bits (70), Expect = 0.091
Identities = 26/69 (37%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +1
Query: 508 PKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPS- 681
P T + S P P T+ TS S PP NST + +T V + SST P S
Sbjct: 264 PPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNST-TPVTPTVPPTSTSSTSTPPPPAST 322
Query: 682 NTTGTLSPP 708
++TGT S P
Sbjct: 323 SSTGTSSSP 331
Score = 31.9 bits (69), Expect = 0.12
Identities = 23/71 (32%), Positives = 32/71 (45%)
Frame = +1
Query: 502 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPS 681
VTP P S S PP +T T S P +++ S T + T +ST P+TP+
Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNST--TPVTPT 412
Query: 682 NTTGTLSPPCT 714
+ S P T
Sbjct: 413 VPPTSSSTPLT 423
Score = 29.1 bits (62), Expect = 0.84
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +1
Query: 502 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 678
+TP P S S+P P T+ + S P T ST S T + SS++ P+TP
Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249
Query: 679 S 681
+
Sbjct: 250 T 250
Score = 29.1 bits (62), Expect = 0.84
Identities = 22/71 (30%), Positives = 31/71 (43%), Gaps = 1/71 (1%)
Frame = +1
Query: 493 LHSVTPKTKPARKSPGSLPPCW-KTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAP 669
L++ TP T P S S W TT T S T+ST +T T ++ +
Sbjct: 598 LYTSTPITSPNSTSSSSTQVSWNSTTPITGTSTSKVTSSTSIPLTSTNRTSTTFTSSTSI 657
Query: 670 LTPSNTTGTLS 702
T S++T T S
Sbjct: 658 STSSSSTATSS 668
Score = 25.8 bits (54), Expect = 7.9
Identities = 19/72 (26%), Positives = 32/72 (44%), Gaps = 2/72 (2%)
Frame = +1
Query: 499 SVTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMT--VSSTVIAPL 672
S P T + + S+PP +T + + PP + S+ S+ + SS+ +
Sbjct: 330 SPLPSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLST 389
Query: 673 TPSNTTGTLSPP 708
+ S TT T PP
Sbjct: 390 STSCTTSTSIPP 401
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 32.7 bits (71), Expect = 0.069
Identities = 23/81 (28%), Positives = 33/81 (40%)
Frame = +1
Query: 562 TTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPSNTTGTLSPPCTKAXXXXXXX 741
+T TS PP T+ ++ T ++T + P T NTT T +PP T
Sbjct: 63 STNITSIVPPPSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTAPPTTHVNSTTTVV 122
Query: 742 XXXXXVL*HLMKIWPPTXTVN 804
V + + PPT VN
Sbjct: 123 PPTTHV--NTTTVVPPTTHVN 141
Score = 31.5 bits (68), Expect = 0.16
Identities = 28/79 (35%), Positives = 37/79 (46%), Gaps = 4/79 (5%)
Frame = +1
Query: 496 HSVTPKTKPARKSPGSLPPCWKTTEF--TSRSCPPRT--NST*SSITRKVLVMTVSSTVI 663
H+ T T P S + TT T+ + PP T NST + + V T +TV+
Sbjct: 77 HNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTAPPTTHVNSTTTVVPPTTHVNT--TTVV 134
Query: 664 APLTPSNTTGTLSPPCTKA 720
P T NTT T+ PP T A
Sbjct: 135 PPTTHVNTT-TVVPPTTHA 152
Score = 30.3 bits (65), Expect = 0.37
Identities = 15/57 (26%), Positives = 27/57 (47%)
Frame = +1
Query: 544 LPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPSNTTGTLSPPCT 714
+PP + T+ + PP T+ ++ + ++T P T N+T T+ PP T
Sbjct: 70 VPPPSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTAPPTTHVNSTTTVVPPTT 126
Score = 29.1 bits (62), Expect = 0.84
Identities = 21/72 (29%), Positives = 37/72 (51%), Gaps = 1/72 (1%)
Frame = +1
Query: 502 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTN-ST*SSITRKVLVMTVSSTVIAPLTP 678
V P + + ++PP T+ T+ + PP T+ +T ++ + ++TV+ P T
Sbjct: 70 VPPPSTSHNSTTTTVPPT--TSMNTTTTVPPTTSLNTTTTTAPPTTHVNSTTTVVPPTTH 127
Query: 679 SNTTGTLSPPCT 714
NTT T+ PP T
Sbjct: 128 VNTT-TVVPPTT 138
>SPAC343.04c |gnr1||heterotrimeric G protein beta subunit
Gnr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 29.5 bits (63), Expect = 0.64
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -1
Query: 584 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 483
DLE N +V Q+ G G+FL G FG + FV+
Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444
>SPCC576.13 |swc5||chromatin remodeling complex subunit
Swc5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 215
Score = 29.1 bits (62), Expect = 0.84
Identities = 15/48 (31%), Positives = 26/48 (54%)
Frame = +3
Query: 228 ETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDGKE 371
ET K S K +K + + +++++ K NT++ A Q W+K KE
Sbjct: 128 ETPKKKHSLIRKRRKSPLDSSSAQKVLKKNKLNTLEQAQQNWSKYIKE 175
>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 28.7 bits (61), Expect = 1.1
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -1
Query: 716 FVHGGLKVPVVFEGVSGAITVDDTVIT 636
F H + V G+ GAIT+DD++IT
Sbjct: 107 FCHLSIGQEAVAAGIEGAITLDDSIIT 133
>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
Dna2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1398
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 3/38 (7%)
Frame = -2
Query: 109 QSHLGAVVIFESKS---TNNIKILXXVPIXSVDRYXGR 5
QS +G + I++S+ + N+K + I +VDRY GR
Sbjct: 1236 QSSIGIISIYKSQVELLSKNLKSFTEIEINTVDRYQGR 1273
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 195 QLYMSVVIGEY-ETAIAKCSEYLKEKKGEVIKEAVKRLIE 311
Q+++++V+ Y A+ EYL+EK I + K ++E
Sbjct: 556 QIFINIVLPNYIRAALVVAKEYLREKANADINDLTKDMLE 595
>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 967
Score = 27.5 bits (58), Expect = 2.6
Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 4/54 (7%)
Frame = -2
Query: 202 YSCSASTSSVLGASVALEA----SAHTARTKAKQSQSHLGAVVIFESKSTNNIK 53
YS + S VL +S EA SAH+A K S+S + +FES ++NN++
Sbjct: 121 YSVAESPVPVLQSSAVSEADDMASAHSAHPSRKASRS----LRLFESSTSNNLE 170
>SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar
membrane transporter Hmt1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 830
Score = 27.5 bits (58), Expect = 2.6
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 182 VISSWCKCGVRSQRTHGEDEGKTKSVSSWRSGY 84
+I + C C V + T E + K V+SWR Y
Sbjct: 412 LIMTLCYCYVTVKITSWRTEARRKMVNSWRESY 444
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.1 bits (57), Expect = 3.4
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +1
Query: 559 KTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPSNTTGTLSPPCT 714
+TTE T+ P T ++ S T ++ T T I P+ P N + ++S P T
Sbjct: 853 ETTEGTATYTEPTTFTSTFSFTTTIIGGT---TTIIPVNPGNPSSSVSAPPT 901
>SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 417
Score = 26.6 bits (56), Expect = 4.5
Identities = 16/76 (21%), Positives = 34/76 (44%)
Frame = -2
Query: 331 MVFLLPFSIRRFTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVAL 152
M F ++ T + +PF R+ HL S +T+ +Y+ + T ++++
Sbjct: 1 MRFFETLALALLTTGALAAPF---RHPHHLLNKRDVSVVTSKVYAYTTVTLEAAASAIST 57
Query: 151 EASAHTARTKAKQSQS 104
+A A T A +++
Sbjct: 58 NGAAKEAATAAGDAET 73
>SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 819
Score = 26.2 bits (55), Expect = 6.0
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1
Query: 661 IAPLTPSNTTGTLSPPCTKA 720
+ PL PS+ +G LSPP KA
Sbjct: 146 VHPLRPSHVSGPLSPPEPKA 165
>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
Smc6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1140
Score = 25.8 bits (54), Expect = 7.9
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +3
Query: 432 LINKRDHHALKLIDQQNHNKIAFGDSKDKTS 524
L+ K+D A K+ DQ H K+ +DK S
Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526
>SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 892
Score = 25.8 bits (54), Expect = 7.9
Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 6/51 (11%)
Frame = +3
Query: 636 SDDRIIYGDSTADTFKHHWYLEP----SMYESDVMFFVYNR--XYNSVMTL 770
SDDR+ GD+ + +Y++ +MYE ++ Y YN V+ L
Sbjct: 84 SDDRVAGGDALPSASQEKYYVQQIHGLAMYEIHTVYVDYKHLTSYNDVLAL 134
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,379,509
Number of Sequences: 5004
Number of extensions: 71523
Number of successful extensions: 307
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 269
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 304
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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