Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_M02
(880 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 304 1e-83
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 147 2e-36
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 30 0.50
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 30 0.50
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 28 2.0
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 27 2.7
SPBC2A9.08c |sec22||SNARE Sec22|Schizosaccharomyces pombe|chr 2|... 27 2.7
SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 27 2.7
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 3.5
SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces pombe... 26 6.1
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 26 6.1
SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.1
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 26 8.1
SPAC8C9.06c |||mitochondrial translation regulator |Schizosaccha... 26 8.1
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 26 8.1
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 26 8.1
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 304 bits (746), Expect = 1e-83
Identities = 140/235 (59%), Positives = 182/235 (77%), Gaps = 1/235 (0%)
Frame = +1
Query: 148 IKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPF 327
++FL+++KPF LPEIA PERK+ F++K+LWT +TL IFLV Q+PL+GI+SSDS+DP
Sbjct: 4 LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVMSQVPLYGIVSSDSSDPL 63
Query: 328 YWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFGM 504
W+R+ILA+NRGTLMELGISPIVTS +++QLL G+++IEV + DR ++ QK +
Sbjct: 64 LWLRMILAANRGTLMELGISPIVTSSMLVQLLVGSQLIEVNMELKSDREMYQLVQKFLAI 123
Query: 505 VITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSGISL 684
+I GQA YV+TGMYG P ++GAG+CLL+I+QL A LIVLLLDELLQKGYGLGSGISL
Sbjct: 124 IIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLAAASLIVLLLDELLQKGYGLGSGISL 183
Query: 685 FIATNICETIVWKAFSPATVNTGRGTEFEGAVIALXHLLATRPDKVRALREAFYR 849
FIAT CE I WKAFSP T + G +FEGAVI +++ T +K AL +AF+R
Sbjct: 184 FIATINCENIFWKAFSPTTYHIANGVQFEGAVINFVYVMFTWDNKAAALYQAFFR 238
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 147 bits (356), Expect = 2e-36
Identities = 77/238 (32%), Positives = 136/238 (57%), Gaps = 3/238 (1%)
Frame = +1
Query: 145 GIKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 324
G +F+ IKP S+LPE+ P+ ++ EK+ W A + ++ + IP++G +D+ DP
Sbjct: 3 GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDP 62
Query: 325 FYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFG 501
RV+ S+ LM G++PI S ++Q+LA K I V + DR LF AQK+
Sbjct: 63 INNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKIAVNFNLIIDRVLFQNAQKVVS 122
Query: 502 MVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 681
++ + A+ YV +G YG S++G +++I+Q+F+ G++ + L E+++KG+GLGSG
Sbjct: 123 ALLYLILAVTYVSSGYYGSFSDLGIFRFIMLILQIFLPGIVCIYLCEIIEKGHGLGSGPV 182
Query: 682 LFIATNICETIVWKAFS--PATVNTGRGTEFEGAVIALXHLLATRPDKVRALREAFYR 849
L + ++I I+W S VN ++++GA++ L + +K +LR +R
Sbjct: 183 LLLGSHILGNIMWDVLSLHRYPVNESGDSQYQGALVGFAFNLFSFKNKFSSLRSILFR 240
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 29.9 bits (64), Expect = 0.50
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = -3
Query: 698 FVAINREIPEPNPYPFWSNSSSNNTIRPATNSCMIMRRHTPAPIS 564
+ +I+RE+ N PF SNS S ++ T++ +R + +PI+
Sbjct: 29 YTSISRELDYANQSPFSSNSFSPTELKARTSATFDVRSASTSPIN 73
>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 346
Score = 29.9 bits (64), Expect = 0.50
Identities = 23/67 (34%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
Frame = -3
Query: 494 NFCAPLNKALSFGVSPTS-MILAPASNCMISPDVTIGEIPSSINV---PLFDARITRIQ* 327
+ CA +NKAL G+ TS A C PD I + S V PL + RI ++
Sbjct: 104 SLCAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRA 160
Query: 326 KGSALSD 306
G L D
Sbjct: 161 SGRVLVD 167
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 27.9 bits (59), Expect = 2.0
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -3
Query: 686 NREIPEPNPYPFWSNSSSNNT 624
N +P P P P +NSS+NNT
Sbjct: 395 NSNMPPPAPRPVTNNSSNNNT 415
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Frame = -2
Query: 684 QRDTRTQSISFLEQ---FIEQQHNKTGHKQLYDNEEAHSGTNFTGFAIHSSHYINNSLTN 514
Q+ +S S ++ F E Q++ TG+K + A +G + G + +S +
Sbjct: 1700 QKSPSVESFSIIDNDNPFHEGQNSSTGYKDIVQGLLAENGVSEAGVDVMTSIVSSTIPIV 1759
Query: 513 SNHHTK 496
SNH T+
Sbjct: 1760 SNHQTE 1765
>SPBC2A9.08c |sec22||SNARE Sec22|Schizosaccharomyces pombe|chr
2|||Manual
Length = 209
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/69 (24%), Positives = 26/69 (37%)
Frame = -2
Query: 714 NGFTDVCSNKQRDTRTQSISFLEQFIEQQHNKTGHKQLYDNEEAHSGTNFTGFAIHSSHY 535
NG +C +Q R + S+LE+ + N G + L ++ F F S
Sbjct: 59 NGICYLCICEQSYPRKLAFSYLEELAGEFWNSFGEEALQPGLRPYAFVQFDTFMQKSKRV 118
Query: 534 INNSLTNSN 508
N N N
Sbjct: 119 YNTPRANDN 127
>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 2.7
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -2
Query: 621 KTGHKQLYDNEEAHSGTNFTGFAIHSSHYINN-SLTNSNHHT 499
+T +Y + E H+G G +HSS NN S T+SN T
Sbjct: 144 QTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKGT 185
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 3.5
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 148 IKFLEVI-KPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMS 306
I+FL+ KP+ S + R++ + K WT + I L+ PLFGI S
Sbjct: 1055 IRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFS 1108
>SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 365
Score = 26.2 bits (55), Expect = 6.1
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = +1
Query: 706 ETIVWKAFSPATVNTGRGTEFEGAVIALXHLLATRPDK 819
E +VW+ F TV TG G I ++L R ++
Sbjct: 273 EDVVWETFDSQTVETGNGELCAANFIPAVYVLNQRKEE 310
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 26.2 bits (55), Expect = 6.1
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -3
Query: 842 KASRRARTLSGRVANRWXNAITAPS 768
K RR+R LS ++ N W + +PS
Sbjct: 542 KLFRRSRELSEKLCNNWSERVKSPS 566
>SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 6.1
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = -3
Query: 374 SINVPLFDARITRIQ*KGSALSDDIIPNKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAI 195
S+ + LFD I+ + S +SD IP K +++ N + T+S N + +S
Sbjct: 273 SLLLGLFDKSISMLNCHSSKISDGDIPYKSHQVFLQELQNKYSEIKTYSSNKLNKSSIKS 332
Query: 194 S 192
S
Sbjct: 333 S 333
>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 772
Score = 25.8 bits (54), Expect = 8.1
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -3
Query: 710 VSQMFVAINREIPEPNPYPFWS-NSSSNNTIRPATNSCMIMRRHTPAPISLGS 555
VS FV+ E PE Y + NSSSN +R + + + P+SL S
Sbjct: 288 VSSTFVSATSE-PEVETYSVSTKNSSSNKNLRSSLSKLLSTSNLNNKPLSLSS 339
>SPAC8C9.06c |||mitochondrial translation regulator
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 931
Score = 25.8 bits (54), Expect = 8.1
Identities = 21/75 (28%), Positives = 32/75 (42%)
Frame = -2
Query: 555 AIHSSHYINNSLTNSNHHTK*FLCTIKQSPVFRSVTNFNDFSTSKQLHDKS*CYNR*DTK 376
A SH N+ N + T+ +S V R+V ND + KS C+ D++
Sbjct: 32 AFQQSHTFNSMSVFKNDNAIANQTTVNESDVKRNVEKINDIYECSN-NTKSPCFPNSDSR 90
Query: 375 LH*CPSV*CKNYTDP 331
+ P V K T+P
Sbjct: 91 I---PLVNWKTSTNP 102
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -3
Query: 197 ISGNILQNGLITSKNFIPILIYC 129
+ G L +GL+ K+F+P L C
Sbjct: 83 VQGEDLDDGLLVDKHFLPTLFKC 105
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 25.8 bits (54), Expect = 8.1
Identities = 18/72 (25%), Positives = 31/72 (43%), Gaps = 5/72 (6%)
Frame = -2
Query: 804 CQQMXQCYHSTLEFCAA-----TSVNSGW*KCLPYNGFTDVCSNKQRDTRTQSISFLEQF 640
C M C EFC T + W C N + + S RD++++S + LE++
Sbjct: 304 CNHMT-CKKCKYEFCWVCLGPWTEHGNNWYTC---NRYEEKSSTSARDSQSKSRASLERY 359
Query: 639 IEQQHNKTGHKQ 604
+ + H+Q
Sbjct: 360 LHYYNRFANHEQ 371
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,614,363
Number of Sequences: 5004
Number of extensions: 81298
Number of successful extensions: 246
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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