Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_L17
(942 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 39 0.001
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.019
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.072
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.24
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.9
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.8
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 23 6.0
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 6.7
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 6.7
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 38.7 bits (86), Expect = 0.001
Identities = 20/53 (37%), Positives = 20/53 (37%)
Frame = -1
Query: 714 LLXKXPPTXPXXXXXPPXXXXXXPFFXPXXPPXXGXTPPPPPPXXXXPXPXPP 556
LL K PP P P P P P G PPPPPP P PP
Sbjct: 727 LLLKSPPPPPPAVIVPTPAPAPIP-VPPPAPIMGGPPPPPPPPGVAGAGPPPP 778
Score = 32.7 bits (71), Expect = 0.077
Identities = 15/30 (50%), Positives = 15/30 (50%), Gaps = 2/30 (6%)
Frame = -3
Query: 670 PPXXPXX*XXFXPPXPPX--GXGXPPPPPP 587
PP P PP PP G G PPPPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 31.1 bits (67), Expect = 0.24
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = -3
Query: 634 PPXPPXGXGXPPPPPPXXXXTXPXXP 557
PP P G PPPPPP P P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPP 778
Score = 29.1 bits (62), Expect = 0.95
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -2
Query: 572 PPPXPPXXXXPPPPPP 525
PPP P PPPPPP
Sbjct: 752 PPPAPIMGGPPPPPPP 767
Score = 28.3 bits (60), Expect = 1.7
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = -2
Query: 569 PPXPPXXXXPPPPPPGXG 516
PP P PPPPPP G
Sbjct: 752 PPPAPIMGGPPPPPPPPG 769
Score = 27.9 bits (59), Expect = 2.2
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = -1
Query: 669 PPXXXXXXPFFXPXXPPXXGXTPPPPPP 586
PP P P P G PPPPPP
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 27.9 bits (59), Expect = 2.2
Identities = 11/18 (61%), Positives = 11/18 (61%), Gaps = 2/18 (11%)
Frame = -2
Query: 572 PPPXPP--XXXXPPPPPP 525
PPP PP PPPPPP
Sbjct: 763 PPPPPPGVAGAGPPPPPP 780
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.7 bits (76), Expect = 0.019
Identities = 14/29 (48%), Positives = 14/29 (48%)
Frame = -3
Query: 673 PPPXXPXX*XXFXPPXPPXGXGXPPPPPP 587
PPP P P PP G PPPPPP
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPP 366
Score = 26.6 bits (56), Expect = 5.1
Identities = 15/49 (30%), Positives = 16/49 (32%), Gaps = 2/49 (4%)
Frame = -1
Query: 699 PPTXPXXXXXPPXXXXXXPFFXPXXPPXXGXTP--PPPPPXXXXPXPXP 559
PP+ P P P PP P PP PP P P P
Sbjct: 432 PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAP 480
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.5 bits (63), Expect = 0.72
Identities = 13/29 (44%), Positives = 14/29 (48%), Gaps = 3/29 (10%)
Frame = -2
Query: 572 PPPXPPXXXXP---PPPPPGXGXXAPKXN 495
PPP PP P PPPPP G + N
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPPGYVKKRKN 38
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = -1
Query: 624 PPXXGXTPPPPPPXXXXPXPXPP 556
PP G PPPPPP P PP
Sbjct: 5 PP--GNPPPPPPPPGFEPPSQPP 25
Score = 27.9 bits (59), Expect = 2.2
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = -1
Query: 621 PXXGXTPPPPPPXXXXPXPXPP 556
P PPPPPP P PP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPP 26
Score = 27.5 bits (58), Expect(2) = 0.072
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = -2
Query: 560 PPXXXXPPPPPPG 522
PP PPPPPPG
Sbjct: 5 PPGNPPPPPPPPG 17
Score = 27.1 bits (57), Expect = 3.8
Identities = 14/29 (48%), Positives = 14/29 (48%)
Frame = -3
Query: 673 PPPXXPXX*XXFXPPXPPXGXGXPPPPPP 587
PPP P F PP P PPPPPP
Sbjct: 9 PPPPPPPP--GFEPPSQP-----PPPPPP 30
Score = 23.8 bits (49), Expect(2) = 0.072
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = -2
Query: 542 PPPPPPGXGXXAP 504
PPPPPP G P
Sbjct: 9 PPPPPPPPGFEPP 21
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 31.1 bits (67), Expect = 0.24
Identities = 15/31 (48%), Positives = 15/31 (48%)
Frame = +1
Query: 481 GGGGGXFFGAXXPXPGGGGGGXXXXGGXGGG 573
GGG G F G GG GG GG GGG
Sbjct: 227 GGGPGGFEGGPGGFGGGPGGFGGGLGGFGGG 257
Score = 28.7 bits (61), Expect = 1.3
Identities = 15/39 (38%), Positives = 15/39 (38%)
Frame = +3
Query: 558 GXXGXVXXXXGGGGGGXPXPXGGXGGKKXXXXXGXXGGG 674
G G GGG GG P GG GG G GG
Sbjct: 184 GHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGG 222
Score = 28.3 bits (60), Expect = 1.7
Identities = 15/32 (46%), Positives = 15/32 (46%), Gaps = 3/32 (9%)
Frame = +1
Query: 481 GGGGGXF---FGAXXPXPGGGGGGXXXXGGXG 567
GGG G F G PGG GGG GG G
Sbjct: 241 GGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPG 272
Score = 27.9 bits (59), Expect = 2.2
Identities = 15/30 (50%), Positives = 16/30 (53%)
Frame = +1
Query: 481 GGGGGXFFGAXXPXPGGGGGGXXXXGGXGG 570
GG GG G+ P PG GG G GG GG
Sbjct: 188 GGFGGFGGGSGGPPPGPGGFGGF--GGFGG 215
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 2.9
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = -1
Query: 624 PPXXGXTPPPPPPXXXXPXPXPP 556
PP PPPPP P P P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAP 1721
Score = 26.6 bits (56), Expect = 5.1
Identities = 13/43 (30%), Positives = 13/43 (30%)
Frame = -3
Query: 715 PXXXXPXNXXXXXXPPPXXPXX*XXFXPPXPPXGXGXPPPPPP 587
P P PPP PP P PPPP P
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 26.6 bits (56), Expect = 5.1
Identities = 15/48 (31%), Positives = 15/48 (31%), Gaps = 1/48 (2%)
Frame = -1
Query: 699 PPTXPXXXXXPPXXXXXXPFFXPXXPPXXGXTPPPPPPXXXXP-XPXP 559
PP PP P P P PPPP P P P P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
Score = 26.6 bits (56), Expect = 5.1
Identities = 12/28 (42%), Positives = 12/28 (42%), Gaps = 2/28 (7%)
Frame = -1
Query: 633 PXXPPXXGXTPPPP--PPXXXXPXPXPP 556
P PP PPPP PP P PP
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732
Score = 26.2 bits (55), Expect = 6.7
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = -1
Query: 624 PPXXGXTPPPPPPXXXXPXPXPP 556
P TPPPPP P PP
Sbjct: 1700 PQMSAPTPPPPPMSVPPPPSAPP 1722
Score = 26.2 bits (55), Expect = 6.7
Identities = 12/25 (48%), Positives = 12/25 (48%), Gaps = 2/25 (8%)
Frame = -2
Query: 572 PPPXPPXXXXPP--PPPPGXGXXAP 504
PP PP PP PPPP AP
Sbjct: 1717 PPSAPPMPAGPPSAPPPPLPASSAP 1741
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.1 bits (57), Expect = 3.8
Identities = 15/47 (31%), Positives = 15/47 (31%)
Frame = -1
Query: 699 PPTXPXXXXXPPXXXXXXPFFXPXXPPXXGXTPPPPPPXXXXPXPXP 559
PP PP P P P PP PPP P P P
Sbjct: 1179 PPVPKPAAGVPPVPP---PSEAPPVPKPSVGVPPVPPPSTAPPVPTP 1222
Score = 26.6 bits (56), Expect = 5.1
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = -3
Query: 634 PPXPPXGXGXPPPPPPXXXXTXP 566
PP P G PP PPP P
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVP 1201
Score = 26.6 bits (56), Expect = 5.1
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = -3
Query: 634 PPXPPXGXGXPPPPPPXXXXTXP 566
PP P G PP PPP P
Sbjct: 1198 PPVPKPSVGVPPVPPPSTAPPVP 1220
Score = 26.2 bits (55), Expect = 6.7
Identities = 10/24 (41%), Positives = 10/24 (41%)
Frame = -2
Query: 572 PPPXPPXXXXPPPPPPGXGXXAPK 501
PP P PP PPP PK
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPK 1202
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 22.6 bits (46), Expect(2) = 6.0
Identities = 8/16 (50%), Positives = 8/16 (50%)
Frame = -2
Query: 572 PPPXPPXXXXPPPPPP 525
P P PPPPPP
Sbjct: 179 PATSLPSDYNPPPPPP 194
Score = 21.8 bits (44), Expect(2) = 6.0
Identities = 8/16 (50%), Positives = 8/16 (50%)
Frame = -2
Query: 542 PPPPPPGXGXXAPKXN 495
PPPPPP A N
Sbjct: 192 PPPPPPAVEDQAADAN 207
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 6.7
Identities = 16/49 (32%), Positives = 16/49 (32%), Gaps = 1/49 (2%)
Frame = -1
Query: 699 PPTX-PXXXXXPPXXXXXXPFFXPXXPPXXGXTPPPPPPXXXXPXPXPP 556
PPT P PP P PP PPP P P PP
Sbjct: 139 PPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQP--AAPVKSPP 185
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 6.7
Identities = 14/30 (46%), Positives = 14/30 (46%), Gaps = 1/30 (3%)
Frame = +1
Query: 484 GGGGXFFGAXXPXPGGG-GGGXXXXGGXGG 570
GG G F G GGG GG GG GG
Sbjct: 23 GGRGGFGGGRGGARGGGRGGARGGRGGRGG 52
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,527,641
Number of Sequences: 5004
Number of extensions: 18825
Number of successful extensions: 360
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 44
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 252
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 479324640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -