BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_L04
(882 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.2
SPBC16A3.05c |rae1||RNA export factor Rae1|Schizosaccharomyces p... 28 1.5
SPAC19D5.01 |pyp2||tyrosine phosphatase Pyp2|Schizosaccharomyces... 28 2.0
SPCC794.11c |||ENTH domain protein Ent3|Schizosaccharomyces pomb... 27 4.7
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 26 6.2
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 28.7 bits (61), Expect = 1.2
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -3
Query: 301 PAFPKSPSSFCPKVPKTLPPPICLSQV--TSXRVSP*GLPA 185
P+ P PS+ P PK PPP+ + V TS R S PA
Sbjct: 185 PSAPSLPSAVPPMPPKVPPPPLSQAPVANTSSRPSSFAPPA 225
>SPBC16A3.05c |rae1||RNA export factor Rae1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 352
Score = 28.3 bits (60), Expect = 1.5
Identities = 22/78 (28%), Positives = 35/78 (44%), Gaps = 5/78 (6%)
Frame = +3
Query: 438 TIDLNRQIGGRSGMTASGS-GVWDLDKNTHFSA----GGMVSKEFGHKRPDVGLQAEIRH 602
+I + Q G S + G+ WD D + + GG +S ++ D+ A I +
Sbjct: 259 SIAFHPQYGTFSTAGSDGTFSFWDKDSHQRLKSYPNVGGTISCSTFNRTGDIFAYA-ISY 317
Query: 603 DW*SPQHLNNNLLPNKLV 656
DW NN LPNK++
Sbjct: 318 DWSKGYTFNNAQLPNKIM 335
>SPAC19D5.01 |pyp2||tyrosine phosphatase Pyp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 711
Score = 27.9 bits (59), Expect = 2.0
Identities = 14/31 (45%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = -3
Query: 292 PKSPS-SFCPKVPKTLPPPICLSQVTSXRVS 203
P SP SF +VP +PPP+C V S S
Sbjct: 165 PISPDYSFPLRVPINIPPPLCTPSVVSDTFS 195
>SPCC794.11c |||ENTH domain protein Ent3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 476
Score = 26.6 bits (56), Expect = 4.7
Identities = 19/53 (35%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = +3
Query: 246 GKVFGTLGQNDDGLFGKAGYNR-EIFNDDRGKLTGQAYGTRVLGPGGDSTNYG 401
GK G +G + D + +R F RG +Y TRV G GG T+YG
Sbjct: 166 GKFIG-VGSDGDSRISTSSKSRFPSFGSSRG-----SYRTRVYGDGGGFTDYG 212
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 26.2 bits (55), Expect = 6.2
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = -3
Query: 532 AEKWVFLSRSHTPEPDAVIPDLPPICLFRSIVACAFLF 419
AEKWV L+ P D V+ + C + F F
Sbjct: 357 AEKWVLLNGQRCPTCDRVVERIDGCCHMNCLCGTHFCF 394
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,281,661
Number of Sequences: 5004
Number of extensions: 69149
Number of successful extensions: 151
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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