Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_L02
(956 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.019
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.18
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 30 0.42
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.55
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 30 0.55
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 28 2.2
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.7 bits (76), Expect = 0.019
Identities = 18/56 (32%), Positives = 20/56 (35%)
Frame = +1
Query: 742 PPXXSPPXGPPXXPXLARXPXPXXPPXXPFGKPGPXPXXPTPXRXXPXXGXXPXXP 909
PP +PP PP P P PP P G P P P+ P P P
Sbjct: 415 PPVPTPPSLPPSAPPSL---PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAP 467
Score = 29.1 bits (62), Expect = 0.97
Identities = 29/129 (22%), Positives = 32/129 (24%), Gaps = 9/129 (6%)
Frame = +3
Query: 597 PPXGQNPXXGXXAGVSLXXPPP*XPSPXPXPXXXGGXXRTGLXXXPGXSPPGK------- 755
PP A PPP PS G + P PP
Sbjct: 293 PPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIP 352
Query: 756 -PPXXAPXXPXPCPXXG-TPGPAXXPLRETXAXXXXPHPXXGXPKXGXKXXPXXXXGXXX 929
PP P P P + G PL + A P P P P
Sbjct: 353 LPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRT 412
Query: 930 KNPPXXPPP 956
PP PP
Sbjct: 413 STPPVPTPP 421
Score = 29.1 bits (62), Expect = 0.97
Identities = 28/100 (28%), Positives = 32/100 (32%), Gaps = 4/100 (4%)
Frame = +3
Query: 543 PGXPKIXGXPXKXXXTKRPPXGQNPXX--GXXAGVSLXXPPP*XPS-PXPXPXXXGGXXR 713
P P+ P P G+ P A + PPP P P G R
Sbjct: 352 PLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASR 411
Query: 714 TGLXXXPGXSPPGKPPXXAPXXPXPCPXXGTPG-PAXXPL 830
T P +PP PP P P P G PA PL
Sbjct: 412 TSTPPVP--TPPSLPPSAPPSLPPSAPPSLPMGAPAAPPL 449
Score = 27.1 bits (57), Expect = 3.9
Identities = 14/38 (36%), Positives = 14/38 (36%), Gaps = 1/38 (2%)
Frame = +1
Query: 757 PPXGPPXXPXLARXPX-PXXPPXXPFGKPGPXPXXPTP 867
PP P P A P P PP P P P P P
Sbjct: 450 PPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAP 487
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.5 bits (68), Expect = 0.18
Identities = 31/148 (20%), Positives = 37/148 (25%), Gaps = 4/148 (2%)
Frame = +3
Query: 525 PPXXGXPGXPKIXGXPXKXXXTKRPPXGQNPXXGXXAGVSLXXPPP*XPSPXPXPXXXGG 704
PP G P + +P P + P P P P P
Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAP-PVPVPSGAPP 1141
Query: 705 XXRTGLXXXPGXSPPGKPPXXAP---XXPXPCPXXGTPGPAXXPLRETXAXXXXPH-PXX 872
+ + P +P G PP P P P P G P P P P
Sbjct: 1142 VPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIP-PVPKPAAGVPPVPPPSEAPPV 1200
Query: 873 GXPKXGXKXXPXXXXGXXXKNPPXXPPP 956
P G P P PP
Sbjct: 1201 PKPSVGVPPVPPPSTAPPVPTPSAGLPP 1228
Score = 28.3 bits (60), Expect = 1.7
Identities = 21/74 (28%), Positives = 22/74 (29%), Gaps = 3/74 (4%)
Frame = +1
Query: 742 PPXXSPPXGPPXXPXLARXPXPXXPP--XXPFGKPG-PXPXXPTPXRXXPXXGXXPXXPX 912
PP +P G P P A P PP P KP P P P P P
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPP 1228
Query: 913 XXAXFXKTPXXNPP 954
K P P
Sbjct: 1229 VPVPTAKAPPVPAP 1242
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.3 bits (65), Expect = 0.42
Identities = 16/38 (42%), Positives = 16/38 (42%), Gaps = 2/38 (5%)
Frame = +1
Query: 754 SPPXGPPXX--PXLARXPXPXXPPXXPFGKPGPXPXXP 861
SPP PP P A P P PP G P P P P
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 29.5 bits (63), Expect = 0.73
Identities = 13/39 (33%), Positives = 15/39 (38%)
Frame = +1
Query: 745 PXXSPPXGPPXXPXLARXPXPXXPPXXPFGKPGPXPXXP 861
P +P PP P + P P PP P P P P
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 26.6 bits (56), Expect = 5.2
Identities = 19/63 (30%), Positives = 21/63 (33%)
Frame = +3
Query: 768 APXXPXPCPXXGTPGPAXXPLRETXAXXXXPHPXXGXPKXGXKXXPXXXXGXXXKNPPXX 947
+P P P TP PA P+ P P G P P G PP
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPV-------PPPAPIMGGP-----PPPPPPPGVAGAGPPPP 778
Query: 948 PPP 956
PPP
Sbjct: 779 PPP 781
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.9 bits (64), Expect = 0.55
Identities = 18/47 (38%), Positives = 20/47 (42%)
Frame = +1
Query: 742 PPXXSPPXGPPXXPXLARXPXPXXPPXXPFGKPGPXPXXPTPXRXXP 882
PP S P PP P ++ P P PP P G P P P P P
Sbjct: 1699 PPQMSAPTPPP--PPMSVPPPPSAPP-MPAGPPS-APPPPLPASSAP 1741
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.9 bits (64), Expect = 0.55
Identities = 19/67 (28%), Positives = 22/67 (32%), Gaps = 1/67 (1%)
Frame = +1
Query: 754 SPPXGP-PXXPXLARXPXPXXPPXXPFGKPGPXPXXPTPXRXXPXXGXXPXXPXXXAXFX 930
+PP P P P P PP P P P P + P P P A
Sbjct: 123 APPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLP-PPAQPAAPV 181
Query: 931 KTPXXNP 951
K+P P
Sbjct: 182 KSPPSAP 188
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 27.9 bits (59), Expect = 2.2
Identities = 17/47 (36%), Positives = 17/47 (36%), Gaps = 1/47 (2%)
Frame = -1
Query: 845 GPGFPKGXXGGXXGXGXRAR-XGXXGGPXGGLXXGGXXRXXXXSRXG 708
GP PKG G G G R G GG GG SR G
Sbjct: 123 GPKKPKGARNGPAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGG 169
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,919,848
Number of Sequences: 5004
Number of extensions: 23389
Number of successful extensions: 103
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 67
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 489310570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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